Thomas Brabletz
University of Erlangen-Nuremberg
CancerInternal medicineWnt signaling pathwayPathologyRegulation of gene expressionMetastasisTumor progressionStem cellCateninCancer researchColorectal cancerCancer stem cellCarcinogenesisSignal transductionMedicineEpithelial–mesenchymal transitionTranscription factorBiologyCell biologyCancer cell
Publications 191
#1Jueqiong Wang (Monash University)H-Index: 2
#2Carlos Farkas (UM: University of Manitoba)H-Index: 2
Last. Thomas Brabletz (FAU: University of Erlangen-Nuremberg)H-Index: 70
view all 15 authors...
The ZEB2 transcription factor has been demonstrated to play important roles in hematopoiesis and leukemic transformation. ZEB1 is a close family member of ZEB2 but has remained more enigmatic concerning its roles in hematopoiesis. Here, we show using conditional loss-of-function approaches and bone marrow (BM) reconstitution experiments that ZEB1 plays a cell-autonomous role in hematopoietic lineage differentiation, particularly as a positive regulator of monocyte development in addition to its ...
#1Simone Brabletz (FAU: University of Erlangen-Nuremberg)H-Index: 15
#2Harald Schuhwerk (FAU: University of Erlangen-Nuremberg)H-Index: 2
Last. Marc P. Stemmler (FAU: University of Erlangen-Nuremberg)H-Index: 22
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The process of epithelial-mesenchymal transition (EMT) is fundamental for embryonic morphogenesis. Cells undergoing it lose epithelial characteristics and integrity, acquire mesenchymal features, and become motile. In cancer, this program is hijacked to confer essential changes in morphology and motility that fuel invasion. In addition, EMT is increasingly understood to orchestrate a large variety of complementary cancer features, such as tumor cell stemness, tumorigenicity, resistance to therap...
2 CitationsSource
#1Bhavana Gupta (Cardiff University)H-Index: 1
#2Adam C. Errington (Cardiff University)H-Index: 22
Last. Florian A. Siebzehnrubl (Cardiff University)H-Index: 26
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Radial glia-like (RGL) stem cells persist in the adult mammalian hippocampus, where they generate new neurons and astrocytes throughout life. The process of adult neurogenesis is well documented, but cell-autonomous factors regulating neuronal and astroglial differentiation are incompletely understood. Here, we evaluate the functions of the transcription factor zinc-finger E-box binding homeobox 1 (ZEB1) in adult hippocampal RGL cells using a conditional-inducible mouse model. We find that ZEB1 ...
#1Sebastian Baasch (University of Freiburg)H-Index: 2
#2Piero Giansanti (TUM: Technische Universität München)H-Index: 16
Last. Simone Brabletz (FAU: University of Erlangen-Nuremberg)H-Index: 15
view all 21 authors...
Cytomegaloviruses (CMVs) have co-evolved with their mammalian hosts for millions of years, leading to remarkable host specificity and high infection prevalence. Macrophages, which already populate barrier tissues in the embryo, are the predominant immune cells at potential CMV entry sites. Here we show that, upon CMV infection, macrophages undergo a morphological, immunophenotypic, and metabolic transformation process with features of stemness, altered migration, enhanced invasiveness, and provi...
The pancreas is comprised of exocrine and endocrine compartments releasing digestive enzymes into the duodenum and regulating blood glucose levels by insulin and glucagon release. Tissue homeostasis is depending on transcription factor networks, involving Ptf1α, Ngn3, Nkx6.1, and Sox9, which are already activated during organogenesis. However, proper organ function is challenged by diets of high sugar and fat content, increasing the risk of type 2 diabetes and other disorders. A detailed underst...
#1Sanith Cheriyamundath (Weizmann Institute of Science)H-Index: 8
#2Anmol Kumar (Weizmann Institute of Science)H-Index: 7
Last. Avri Ben-Ze'ev (Weizmann Institute of Science)H-Index: 79
view all 5 authors...
The overactivation of Wnt/β-catenin signaling is a hallmark of colorectal cancer (CRC) development. We identified the cell adhesion molecule L1CAM (L1) as a target of β-catenin-TCF transactivation in CRC cells. The overexpression of L1 in CRC cells confers enhanced proliferation, motility, tumorigenesis and liver metastasis, and L1 is exclusively localized in the invasive areas of human CRC tissue. A number of genes are induced after L1 transfection into CRC cells by a mechanism involving the cy...
Citrate is important for lipid synthesis and epigenetic regulation in addition to ATP production. We have previously reported that cancer cells import extracellular citrate via the pmCiC transporter to support their metabolism. Here, we show for the first time that citrate is supplied to cancer by cancer-associated stroma (CAS) and also that citrate synthesis and release is one of the latter's major metabolic tasks. Citrate release from CAS is controlled by cancer cells through cross-cellular co...
1 CitationsSource
#1Manuel Ruh (FAU: University of Erlangen-Nuremberg)H-Index: 1
#2Marc P. Stemmler (FAU: University of Erlangen-Nuremberg)H-Index: 22
Last. Simone Brabletz (FAU: University of Erlangen-Nuremberg)H-Index: 15
view all 15 authors...
Osteosarcoma is an often-fatal mesenchyme-derived malignancy in children and young adults. Overexpression of EMT-transcription factors (EMT-TFs) has been associated with poor clinical outcome. Here, we demonstrated that the EMT-TF ZEB1 is able to block osteoblastic differentiation in normal bone development as well as in osteosarcoma cells. Consequently, overexpression of ZEB1 in osteosarcoma characterizes poorly differentiated, highly metastatic subgroups and its depletion induces differentiati...
1 CitationsSource
#1Paradesi Naidu Gollavilli (FAU: University of Erlangen-Nuremberg)H-Index: 4
#2Beatrice Parma (FAU: University of Erlangen-Nuremberg)H-Index: 1
Last. Christian Pilarsky (FAU: University of Erlangen-Nuremberg)H-Index: 63
view all 13 authors...
Since their discovery, microRNAs (miRNAs) have been widely studied in almost every aspect of biology and medicine, leading to the identification of important gene regulation circuits and cellular mechanisms. However, investigations are generally focused on the analysis of their downstream targets and biological functions in overexpression and knockdown approaches, while miRNAs endogenous levels and activity remain poorly understood. Here, we used the cellular plasticity-regulating process of epi...
2 CitationsSource
#1Alhomidi Almotiri (Cardiff University)H-Index: 1
#2Hamed Ahmad A Alzahrani (Cardiff University)H-Index: 1
Last. Neil P. Rodrigues (Cardiff University)H-Index: 16
view all 21 authors...
Zeb1, a zinc finger E-box binding homeobox epithelial-mesenchymal (EMT) transcription factor, confers properties of ‘stemness’, such as self-renewal, in cancer. Yet little is known about the function of Zeb1 in adult stem cells. Here, we used the hematopoietic system, as a well-established paradigm of stem cell biology, to evaluate Zeb1 mediated regulation of adult stem cells. We employed a conditional genetic approach using the Mx1-Cre system to specifically knockout (KO) Zeb1 in adult hematopo...
2 CitationsSource