Shinsuke Ohba
University of Tokyo
Induced pluripotent stem cellRegenerative medicineEndocrinologyEmbryonic stem cellCellular differentiationMolecular biologyEndochondral ossificationChemistryOsteoarthritisRUNX2OsteoblastCartilageChondrocyteBone regenerationBone morphogenetic proteinChondrogenesisBone healingTranscription factorBiologyCell biology
Publications 113
#1Shoichiro Tani (UTokyo: University of Tokyo)H-Index: 2
#2Hiroyuki Okada (UTokyo: University of Tokyo)H-Index: 16
Last. Hironori Hojo (UTokyo: University of Tokyo)H-Index: 16
view all 5 authors...
Skeletal disorders, such as osteoarthritis and bone fractures, are among the major conditions that can compromise the quality of daily life of elderly individuals. To treat them, regenerative therapies using skeletal cells have been an attractive choice for patients with unmet clinical needs. Currently, there are two major strategies to prepare the cell sources. The first is to use induced pluripotent stem cells (iPSCs) or embryonic stem cells (ESCs), which can recapitulate the skeletal developm...
#1Akira Yamakawa (UTokyo: University of Tokyo)H-Index: 5
#2Hironori Hojo (UTokyo: University of Tokyo)H-Index: 16
Last. Shinsuke Ohba (UTokyo: University of Tokyo)H-Index: 32
view all 3 authors...
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a powerful tool to identify binding profiles of transcriptional regulators and chromatin regulators as well as histone modification patterns in a genome-wide manner. ChIP-seq consists of five major steps: (1) preparation of cells and chromatin, (2) ChIP, (3) ChIP-seq library construction, (4) sequencing of ChIP DNA with a next-generation sequencer (NGS), and (5) computational analysis of sequence data. Recent ChIP-seq studies in ...
#1Daiki Yamakawa (TMU: Tokyo Medical University)H-Index: 1
#2Yoko Kawase-Koga (TMU: Tokyo Medical University)H-Index: 7
Last. Daichi Chikazu (TMU: Tokyo Medical University)H-Index: 18
view all 9 authors...
#1Sakura Uto (UTokyo: University of Tokyo)H-Index: 4
#2Atsuhiko Hikita (UTokyo: University of Tokyo)H-Index: 17
Last. Kazuto Hoshi (UTokyo: University of Tokyo)H-Index: 36
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2 CitationsSource
#1Shoichiro Tani (UTokyo: University of Tokyo)H-Index: 2
#2Ung-il Chung (UTokyo: University of Tokyo)H-Index: 63
Last. Hironori Hojo (UTokyo: University of Tokyo)H-Index: 16
view all 4 authors...
Pluripotent stem cells (PSCs) are attractive regenerative therapy tools for skeletal tissues. However, a deep understanding of skeletal development is required in order to model this development with PSCs, and for the application of PSCs in clinical settings. Skeletal tissues originate from three types of cell populations: the paraxial mesoderm, lateral plate mesoderm, and neural crest. The paraxial mesoderm gives rise to the sclerotome mainly through somitogenesis. In this process, key developm...
5 CitationsSource
#1Shoko Onodera (UTokyo: University of Tokyo)H-Index: 7
#2Akiko Saito (Tokyo Dental College)H-Index: 9
Last. Shinsuke Ohba (Nagasaki University)H-Index: 32
view all 16 authors...
Summary Two genetic diseases, Gorlin syndrome and McCune-Albright syndrome (MAS), show completely opposite symptoms in terms of bone mineral density and hedgehog (Hh) activity. In this study, we utilized human induced pluripotent stem cell (iPSC)-based models of the two diseases to understand the roles of Hh signaling in osteogenesis. Gorlin syndrome-derived iPSCs showed increased osteoblastogenesis and mineralization with Hh signaling activation and upregulation of a set of transcription factor...
4 CitationsSource
#1Hironori Hojo (UTokyo: University of Tokyo)H-Index: 16
#2Shinsuke Ohba (Nagasaki University)H-Index: 32
Abstract The development of osteoblasts, a bone-forming cell population, occurs in conjunction with development of the skeleton, which creates our physical framework and shapes the body. In the past two decades, genetic studies have uncovered the molecular framework of this process—namely, transcriptional regulators and signaling pathways coordinate the cell fate determination and differentiation of osteoblasts in a spatial and temporal manner. Recently emerging genome-wide studies provide addit...
#1Qing Jiang (Nagasaki University)H-Index: 5
#2Xin Qin (Nagasaki University)H-Index: 5
Last. Toshihisa Komori (Nagasaki University)H-Index: 58
view all 10 authors...
Antxr1/Tem8 is highly expressed in tumor endothelial cells and is a receptor for anthrax toxin. Mutation of Antxr1 causes GAPO syndrome, which is characterized by growth retardation, alopecia, pseudo-anodontia, and optic atrophy. However, the mechanism underlying the growth retardation remains to be clarified. Runx2 is essential for osteoblast differentiation and chondrocyte maturation and regulates chondrocyte proliferation through Ihh induction. In the search of Runx2 target genes in chondrocy...
5 CitationsSource
#1Denise Zujur (UTokyo: University of Tokyo)H-Index: 4
#2Kosuke Kanke (UTokyo: University of Tokyo)H-Index: 3
Last. Shinsuke Ohba (UTokyo: University of Tokyo)H-Index: 32
view all 16 authors...
Abstract Clinically relevant human induced pluripotent stem cell (hiPSC) derivatives require efficient protocols to differentiate hiPSCs into specific lineages. Here we developed a fully defined xeno-free strategy to direct hiPSCs toward osteoblasts within 21 days. The strategy successfully achieved the osteogenic induction of four independently derived hiPSC lines by a sequential use of combinations of small-molecule inducers. The induction first generated mesodermal cells, which subsequently r...
3 CitationsSource
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