Yi-Hua Jan
Rutgers University
ReductaseDNA damageCytochrome P450 reductaseEnzymeChemistryCofactorSelenocysteineTetrahydrobiopterinOxidative stressHemeSepiapterin reductaseMetaboliteSepiapterinThioredoxin reductaseAntioxidantAcetaminophenMethoxsalenCysteineGrowth inhibitionDihydrobiopterinSulfur mustardCytochromeMenadionePsoralenPhotosensitizerThioredoxin-Disulfide ReductaseUltraviolet lightThioredoxinCytochrome P450BiochemistryRedoxMetabolismBiologyCytosol
18Publications
8H-index
268Citations
Publications 18
Newest
#1Yi-Hua Jan (RU: Rutgers University)H-Index: 8
#2Diane E. Heck (NYMC: New York Medical College)H-Index: 4
Last. Jeffrey D. Laskin (RU: Rutgers University)H-Index: 1
view all 4 authors...
Abstract Mustard vesicants, including sulfur mustard (2,2′-dichlorodiethyl sulfide, SM) and nitrogen mustard (bis(2-chloroethyl)methylamine, HN2) are cytotoxic blistering agents synthesized for chemical warfare. Because they contain highly reactive electrophilic chloroethyl side chains, they readily react with cellular macromolecules like DNA forming monofunctional and bifunctional adducts. By targeting DNA, mustards can compromise genomic integrity, disrupt the cell cycle, and cause mutations a...
2 CitationsSource
#1Diane E. Heck (NYMC: New York Medical College)H-Index: 38
#2Laurie B. Joseph (RU: Rutgers University)H-Index: 12
Last. Hong-Duck KimH-Index: 14
view all 7 authors...
Both inorganic mercury and organic mercury compounds, including methyl mercury and ethyl mercury, are noxious pollutants known to cause deleterious neurological and cardiovascular effects in humans and profoundly affect the brains and development of infants and children (for review see Bernhoft RA. J Environ Public Health, 2012; Jung-Duck Park, Wei Zheng. J Prev Med Public Health. 45(6):344–352, 2012; Charles TD, Robert PM, Hing MC, Daniel JJ, Nicola P. J Prev Med Public Health 45(6):344–352, 20...
Source
#1Christophe Guillon (Lehigh University)H-Index: 8
#2Yi-Hua Jan (RU: Rutgers University)H-Index: 8
Last. Ned D. Heindel (Lehigh University)H-Index: 20
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Abstract Linear furocoumarins, also known as psoralens, are clinically useful photo-activated pharmaceuticals employed to address hyperproliferative skin diseases. Seven diverse cytotoxic pharmacophores have been synthetically attached to 8-methoxypsoralen via a 5-amino functionality. The resulting unique set of compounds was evaluated for dark and light toxicity against PAM212 keratinocytes in culture.
3 CitationsSource
#1John T. Szilagyi (RU: Rutgers University)H-Index: 4
#2Karma C. Fussell (RU: Rutgers University)H-Index: 3
Last. Jeffrey D. Laskin (RU: Rutgers University)H-Index: 69
view all 11 authors...
Abstract NADH cytochrome b5 reductase mediates electron transfer from NADH to cytochrome b5 utilizing flavin adenine dinucleotide as a redox cofactor. Reduced cytochrome b5 is an important cofactor in many metabolic reactions including cytochrome P450-mediated xenobiotic metabolism, steroid biosynthesis and fatty acid metabolism, hemoglobin reduction, and methionine and plasmalogen synthesis. Using recombinant human enzyme, we discovered that cytochrome b5 reductase mediates redox cycling of a v...
2 CitationsSource
#1Christophe Guillon (Lehigh University)H-Index: 8
#2Yi-Hua Jan (RU: Rutgers University)H-Index: 8
Last. Ned D. Heindel (Lehigh University)H-Index: 20
view all 9 authors...
The natural product 8-methoxypsoralen (methoxsalen or 8-MOP) in combination with long wavelength ultraviolet light (UVA, 320-400 nm), also referred to as PUVA therapy, is used for the treatment of cutaneous proliferative disorders including psoriasis, vitiligo and mycosis fungoides. The use of 8-MOP (3) is limited by its poor water solubility and there remains a need to develop more water-soluble psoralens to enhance bioavailability following oral administration of the drug. In the present studi...
#1Jeffrey D. Laskin (RU: Rutgers University)H-Index: 69
#2Yi-Hua Jan (RU: Rutgers University)H-Index: 8
Last. Ned D. Heindel (Lehigh University)H-Index: 20
view all 7 authors...
: Photosensitizers are used in the treatment of epidermal proliferation and differentiation disorders such as psoriasis and vitiligo. In these studies, a ring-expanded carbon homolog of the linear psoralen (furo[3,2-g]benzopyran-7-one) class of photosensitizers, 4,10-dimethyl-2H,8H-benzo[1,2-b:5,4-b']dipyran-2-one (NDH2476), was synthesized and analyzed for biological activity. Following activation by ultraviolet light (UVA, 320-400 nm), NDH2476 was found to be a potent inhibitor of keratinocyte...
2 CitationsSource
#1Shaojun Yang (RU: Rutgers University)H-Index: 3
#2Yi-Hua Jan (RU: Rutgers University)H-Index: 8
Last. Jeffrey D. Laskin (RU: Rutgers University)H-Index: 69
view all 6 authors...
Reactive carbonyls such as diacetyl (2,3-butanedione) and 2,3-pentanedione in tobacco and many food and consumer products are known to cause severe respiratory diseases. Many of these chemicals are detoxified by carbonyl reductases in the lung, in particular, dicarbonyl/l-xylulose reductase (DCXR), a multifunctional enzyme important in glucose metabolism. DCXR is a member of the short-chain dehydrogenase/reductase (SDR) superfamily. Using recombinant human enzyme, we discovered that DCXR mediate...
10 CitationsSource
#1John T. Szilagyi (RU: Rutgers University)H-Index: 4
#2Vladimir Mishin (RU: Rutgers University)H-Index: 18
Last. Jeffrey D. Laskin (RU: Rutgers University)H-Index: 69
view all 9 authors...
Cytochrome P450 (CYP) enzymes mediate mixed-function oxidation reactions important in drug metabolism. The aromatic heterocyclic cation, diphenyleneiodonium (DPI), binds flavin in cytochrome P450 reductase and inhibits CYP-mediated activity. DPI also inhibits CYP by directly interacting with heme. Herein, we report that DPI effectively inhibits a number of CYP-related monooxygenase reactions including NADPH oxidase, a microsomal enzyme activity that generates hydrogen peroxide in the absence of ...
7 CitationsSource
#1Yi-Hua Jan (RU: Rutgers University)H-Index: 8
#2Diane E. Heck (NYMC: New York Medical College)H-Index: 38
Last. Jeffrey D. Laskin (RU: Rutgers University)H-Index: 69
view all 5 authors...
The thioredoxin (Trx) system, which consists of Trx and thioredoxin reductase (TrxR), is a major cellular disulfide reduction system important in antioxidant defense. TrxR is a target of mechlorethamine (methylbis(2-chloroethyl)amine; HN2), a bifunctional alkylating agent that covalently binds to selenocysteine/cysteine residues in the redox centers of the enzyme, leading to inactivation and toxicity. Mammalian Trx contains two catalytic cysteines; herein, we determined if HN2 also targets Trx. ...
8 CitationsSource
#1Yi-Hua Jan (RU: Rutgers University)H-Index: 8
#2Jason R. Richardson (RU: Rutgers University)H-Index: 28
Last. Jeffrey D. Laskin (RU: Rutgers University)H-Index: 69
view all 7 authors...
Parathion, a widely used organophosphate insecticide, is considered a high priority chemical threat. Parathion toxicity is dependent on its metabolism by the cytochrome P450 system to paraoxon (diethyl 4-nitrophenyl phosphate), a cytotoxic metabolite. As an effective inhibitor of cholinesterases, paraoxon causes the accumulation of acetylcholine in synapses and overstimulation of nicotinic and muscarinic cholinergic receptors, leading to characteristic signs of organophosphate poisoning. Inhibit...
16 CitationsSource