Guowen Liu
Jilin University
Internal medicineEndocrinologyProinflammatory cytokineChemistryIn vitroOxidative stressKetosisFatty liverNEFALipid metabolismFatty acid synthaseFatty acidBiochemistryOxidative phosphorylationSignal transductionVery low-density lipoproteinBiologyCell biologyProtein kinase APharmacology
129Publications
27H-index
2,279Citations
Publications 123
Newest
#1Jianguo Wang (NWAFU: Northwest A&F University)H-Index: 10
#2Xiaoyan Zhu (French Institute of Health and Medical Research)H-Index: 8
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 6 authors...
An elevated plasma non-esterified fatty acids (NEFA) concentration is an important indicator of negative energy balance, which is often associated with metabolic diseases such as fatty liver and ketosis. Angiopoietin-like protein 4 (ANGPTL4) is a hepatokine that plays important roles in the regulation of lipid metabolism. However, the direct effects of high concentrations of NEFA on ANGPTL4 expression and secretion in hepatocytes from cows are not entirely clear. The objective of this study was ...
8 CitationsSource
#1Xiaoxia Shi (JLU: Jilin University)H-Index: 8
#2Dangdang Li (JLU: Jilin University)H-Index: 3
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 11 authors...
Abstract Non-esterified fatty acids (NEFAs) are important induction factors of inflammatory responses in some metabolic diseases. High plasma levels of NEFAs and oxidative stress exist in the dairy cows with ketosis. The aim of this study was to investigate whether high levels of NEFAs can induce inflammatory response and the specific molecular mechanism in the hepatocytes of dairy cow. In vitro , primary cultured bovine hepatocytes were treated with different concentrations of NEFAs, PDTC (an N...
53 CitationsSource
#1Min ZhangH-Index: 6
#2Shiqi ZhangH-Index: 2
Last. Xinwei LiH-Index: 23
view all 10 authors...
Background/Aims: In dairy cows, β-hydroxybutyrate (BHBA) is utilized as precursors of de novo synthesized fatty acids in mammary gland. Ketotic cows are characterized by excessive negative energy balance (NEB), which can further increase the blood BHBA concentration. Sterol regulatory element-binding protein1 (SREBP1) and cell death-inducing DNA fragmentation factor-alpha-like effector α (Cidea) play crucial roles in lipid synthesis. Therefore, we hypothesized that BHBA could stimulate SREBP1/Ci...
21 CitationsSource
#1Xinwei Li (JLU: Jilin University)H-Index: 23
#2Yu Li (JLU: Jilin University)H-Index: 8
Last. Xiaobing Li (JLU: Jilin University)H-Index: 22
view all 10 authors...
Abstract The natural incidence of fatty liver in ruminants is significantly higher than in monogastric animals. Fatty liver is associated with sterol regulatory element-binding protein 1c (SREBP-1c). The aim of this study was to investigate the regulatory network effects of SREBP-1c on the lipid metabolic genes involved in fatty acid uptake, activation, oxidation, synthesis, and very low-density lipoprotein (VLDL) assembly in bovine hepatocytes. In vitro , bovine hepatocytes were transfected wit...
58 CitationsSource
#1Jianguo Wang (NWAFU: Northwest A&F University)H-Index: 10
#2Xiaoyan Zhu (French Institute of Health and Medical Research)H-Index: 8
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 6 authors...
The objective of this study was to determine the levels of serum copper and zinc in subclinically hypocalcemic peripartum dairy cows in comparison to healthy animals. Blood samples were taken from 219 multiparous Holstein cows near parturition (from 4 weeks prepartum to 4 weeks postpartum) and 51 cows with subclinical hypocalcemia. The results showed that the serum copper concentration increased gradually at 1 week prepartum and remained high for the first 4 weeks postpartum in the healthy perip...
1 CitationsSource
#1Qinghua Deng (JLU: Jilin University)H-Index: 8
#2Xinwei LiH-Index: 23
Last. Xiaobing LiH-Index: 22
view all 13 authors...
Background: Fatty liver is a major metabolic disorder that occurs during early lactation in high-producing dairy cows. Sterol regulatory element-binding protein-1c (SREBP-1c) is an important transcription factor that regulates lipid synthesis by regulating the expression of lipid metabolism genes. Methods: In this study, we reduced the expression of SREBP-1c by adenovirus-mediated SREBP-1c with a low expression vector (AD-GFP-SREBP-1c) to study the effects of SREBP-1c on lipid deposits in bovine...
18 CitationsSource
#1Shoupeng Fu (JLU: Jilin University)H-Index: 19
#2Su-Nan Li (JLU: Jilin University)H-Index: 6
Last. Juxiong Liu (JLU: Jilin University)H-Index: 16
view all 13 authors...
β-Hydroxybutyric acid (BHBA) has neuroprotective effects, but the underlying molecular mechanisms are unclear. Microglial activation plays an important role in neurodegenerative diseases by producing several proinflammatory enzymes and proinflammatory cytokines. The current study investigates the potential mechanisms whereby BHBA affects the expression of potentially proinflammatory proteins by cultured murine microglial BV-2 cells stimulated with lipopolysaccharide (LPS). The results showed tha...
59 CitationsSource
#1Yuxiang Song (JLU: Jilin University)H-Index: 8
#2Xinwei Li (JLU: Jilin University)H-Index: 23
Last. Zhe Wang (JLU: Jilin University)H-Index: 26
view all 10 authors...
A high plasma concentration of non-esterified fatty acids (NEFAs) is an important pathogenic factor that leads to ketosis and fatty liver in dairy cows. NEFAs may be associated with oxidative stress in dairy cows with ketosis or fatty liver and the subsequent induction of hepatocyte damage. However, the molecular mechanism of NEFAs-induced oxidative stress and whether NEFAs cause apoptosis of hepatocytes are unclear. Therefore, the aim of this study was to investigate the molecular mechanism of ...
55 CitationsSource
#1Lei Liu (JLU: Jilin University)H-Index: 13
#2Xinwei Li (JLU: Jilin University)H-Index: 23
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 13 authors...
Abstract High serum concentrations of nonesterified fatty acids (NEFA), which may affect the synthesis and assembly of very low density lipoproteins (VLDL), are associated with fatty liver during the early lactation period. Therefore, the objective of this study was to investigate the effects of NEFA on the synthesis and assembly of VLDL in bovine hepatocytes. Bovine hepatocytes were cultured and treated with different concentrations of NEFA. The mRNA expression of apolipoprotein B100 ( ApoB100 ...
27 CitationsSource
#1Miao Long (Shenyang Agricultural University)H-Index: 16
#2W.J. FengH-Index: 1
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 11 authors...
The aim of this study was to examine the effects of the acid-tolerant engineered bacterial strain Megasphaera elsdenii H6F32 (M. elsdenii H6F32) on ruminal pH and the lactic acid concentrations in simulated rumen acidosis conditions in vitro. A mixed culture of ruminal bacteria, buffer, and primarily degradable substrates was inoculated with equal numbers of M. elsdenii H6 or M. elsdenii H6F32. The pH and lactic acid concentrations in the mixed culture were determined at 0, 2, 4, 6, 8, 10, 12, 1...
11 CitationsSource