Kazuhiro Yamamoto
University of Liverpool
Matrix metalloproteinaseBiophysicsLRP1CellDegradation (geology)Molecular biologyThrombospondinAutofluorescenceProteasesChemistryExtracellular matrixAggrecanAggrecanaseOsteoarthritisADAMTSArthritisCollagenaseCartilageEndocytosisExtracellularBiochemistryFibronectinBiologyCell biology
Publications 44
#1Seandean Lykke Harwood (Novo Nordisk)H-Index: 2
#2Nadia Sukusu Nielsen (AU: Aarhus University)H-Index: 6
Last. Jan J. Enghild (AU: Aarhus University)H-Index: 75
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Abstract null null Human α2-macroglobulin (A2M) is an abundant protease inhibitor in plasma which regulates many proteolytic processes and is involved in innate immunity. A2M’s unique protease-trapping mechanism of inhibition is initiated when a protease cleaves within the exposed and highly susceptible “bait region”. As the wildtype bait region is permissive to cleavage by most human proteases, A2M is accordingly a broad-spectrum protease inhibitor. In this study, we extensively modified the ba...
#1I. CollinsH-Index: 2
#2Kazuhiro YamamotoH-Index: 17
Last. A.K. WannH-Index: 13
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#1Karanvir Saini (UPenn: University of Pennsylvania)H-Index: 4
#2Tiwari M (UPenn: University of Pennsylvania)
Last. Dennis E. Discher (UPenn: University of Pennsylvania)H-Index: 99
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Collagen, the most abundant protein in mammals, contributes to the physical properties of different tissues during development, homeostasis, and disease. The adaptation of physical properties of tissues to mechanical stimuli is thus dependent on the control of tissue collagen levels by well-regulated synthesis and degradation of collagen. Importantly, how various molecular-level events within a tissue sustaining a range of mechanical strains contribute towards maintaining its collagen levels, re...
#1Kazuhiro Yamamoto (University of Liverpool)H-Index: 17
#2David J. Wilkinson (University of Liverpool)H-Index: 11
Last. George Bou-Gharios (University of Liverpool)H-Index: 51
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Metalloproteinases were first identified as collagen cleaving enzymes and are now appreciated to play important roles in a wide variety of biological processes. The aberrant activity and dysregulation of the metalloproteinase family are linked to numerous diseases including cardiovascular and pulmonary diseases, chronic wounds, cancer, fibrosis and arthritis. Osteoarthritis (OA) is the most prevalent age-related joint disorder that causes pain and disability, but there are no disease-modifying d...
1 CitationsSource
#1Lee D. Troughton (University of Liverpool)H-Index: 5
#2Valentina Iorio (University of Liverpool)H-Index: 4
Last. Kevin J. Hamill (University of Liverpool)H-Index: 17
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Abstract Laminin N-terminus α31 (LaNt α31), a member of the laminin superfamily, expressed at low levels in intact epithelium but upregulated during wound repair. Increased expression of LaNt α31 reduced migration rate of corneal keratinocytes through an unknown mechanism. Here, we investigated whether LaNt α31 influences cell behaviour through modulating laminin-mediated processes. Adenoviral delivery of LaNt α31 into corneal epithelial cells led to reduced migration speed and increased cell sp...
4 CitationsSource
#1Karanvir SainiH-Index: 4
#2Manu TewariH-Index: 5
Last. Dennis E. DischerH-Index: 99
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#1Salvatore Santamaria (Imperial College London)H-Index: 14
#2Kazuhiro Yamamoto (University of Liverpool)H-Index: 17
: Aggrecan is a major matrix component of articular cartilage, and its dysregulated proteolysis is a crucial event in the pathogenesis of arthritis. Aggrecanases, members of ADAMTS family, play a pivotal role in aggrecan degradation with ADAMTS-4 and ADAMTS-5 being key enzymes. Cleavage events mediated by ADAMTSs are highly specific and very well characterized; therefore, it is possible to investigate aggrecanolysis by using antibodies reacting with the new N- and C-termini of the cleavage produ...
5 CitationsSource
#1Ursula Mirastschijski (University of Bremen)H-Index: 20
#2Blaž Lupše (University of Bremen)H-Index: 1
Last. Magnus S. ÅgrenH-Index: 42
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Inflammatory processes in the skin augment collagen degradation due to the up-regulation of matrix metalloproteinases (MMPs). The aim of the present project was to study the specific impact of MMP-3 on collagen loss in skin and its interplay with the collagenase MMP-13 under inflammatory conditions mimicked by the addition of the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α). Skin explants from MMP-3 knock-out (KO) mice or from transgenic (TG) mice overexpressing MMP-3 in the skin an...
8 CitationsSource
#1Carsten Scavenius (AU: Aarhus University)H-Index: 17
#2Emil Poulsen (AU: Aarhus University)H-Index: 3
Last. Jan J. Enghild (AU: Aarhus University)H-Index: 75
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Destruction of the cartilage matrix in joints is an important feature of arthritis. Proteolytic degradation of cartilage glycoproteins can contribute to the loss of matrix integrity. Human inter-α-inhibitor (IαI), which stabilizes the extracellular matrix, is composed of the light-chain serine proteinase inhibitor bikunin and two homologous heavy chains (HC1 and HC2) covalently linked through chondroitin 4-sulfate. Inflammation promotes the transfer of HCs from chondroitin 4-sulfate to hyalurona...
3 CitationsSource