Sunao Sugita
Tokyo Medical and Dental University
Induced pluripotent stem cellOphthalmologyInterleukin 21PathologyCytotoxic T cellChemistryImmunologyT cellUveitisRetinaRetinal pigment epitheliumRetinalTransplantationPolymerase chain reactionAntigen-presenting cellMedicineIL-2 receptorBiologyImmune systemCell biology
Publications 171
#1Satoko NakanoH-Index: 8
#2Yasuhiro TomaruH-Index: 4
Last. Sunao SugitaH-Index: 40
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#1Tadao MaedaH-Index: 36
#2Sunao SugitaH-Index: 40
Last. Masayo TakahashiH-Index: 46
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Age-related macular degeneration (AMD) is a highly prevalent irreversible impairment in the elderly population worldwide. Stem cell therapies have been considered potentially viable for treating AMD through the direct replacement of degenerated cells or secretion of trophic factors that facilitate the survival of existing cells. Among them, the safety of pluripotent stem cell-derived retinal pigment epithelial (RPE) cell transplantation against AMD, and some hereditary retinal degenerative disea...
#1Suguru YamasakiH-Index: 5
#2Sunao SugitaH-Index: 40
Last. Michiko MandaiH-Index: 38
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Summary ESC- and iPSC-derived retinal transplantation is a promising therapeutic approach for disease with end-stage retinal degeneration, such as retinitis pigmentosa and age-related macular degeneration. We previously showed medium- to long-term survival, maturation, and light response of transplanted human ESC- and iPSC-retina in mouse, rat, and monkey models of end-stage retinal degeneration. Because the use of patient hiPSC-derived retina with a disease-causing gene mutation is not appropri...
In an intraocular inflammatory state, microglia residing in the retina become active and migrate inside the retina. In this study, we investigated whether cyclooxygenase-1 (COX-1) expressed by retinal microglia/macrophage can be a biomarker for the diagnosis of retinal diseases. COX-1 was immunopositive in microglia/macrophage and neutrophils, while COX-2 was immunopositive in astrocytes and neurons in the inner layer of normal retina. The number of COX-1 positive cells per section of the retina...
#1Masaaki IshidaH-Index: 2
#1Masaaki Ishida (Kyoto University)H-Index: 3
Last. Masayo TakahashiH-Index: 46
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Currently, retinal pigment epithelium (RPE) transplantation includes sheet and single-cell transplantation, the latter of which includes cell death and may be highly immunogenic, and there are some issues to be improved in single-cell transplantation. Y-27632 is an inhibitor of Rho-associated protein kinase (ROCK), the downstream kinase of Rho. We herein investigated the effect of Y-27632 in vitro on retinal pigment epithelium derived from induced pluripotent stem cells (iPS-RPE cells), and also...
1 CitationsSource
#1Kazuichi MaruyamaH-Index: 31
#2Kazuhito YonedaH-Index: 9
Last. Kohji NishidaH-Index: 55
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Cytotoxic T lymphocyte antigen-2 (CTLA-2) alpha has been reported to suppress the activities of cathepsin L (Cath L), which is deeply involved in angiogenesis. Therefore, we assessed whether CTLA-2 alpha plays a role in angiogenesis in ocular tissue. To establish models of corneal inflammation and experimental choroidal neovascularization (CNV), male C57BL/6J mice (n = 5) underwent corneal suture placement or laser-induced CNV, respectively. Mice were then injected with recombinant CTLA-2 alpha ...
Abstract Retinal pigment epithelial (RPE) cells have several functions, including support of the neural retina and choroid in the eye and immunosuppression. Cultured human RPE cells directly suppress inflammatory immune cells. For instance, they directly suppress the activation of T cells in vitro. In contrast, transplanted allogeneic human RPE cells are rejected by bystander immune cells such as T cells in vivo. Recently, human embryonic stem cell-derived RPE cells have been used in several cli...
1 CitationsSource
#1Sunao SugitaH-Index: 40
#2Hiroshi Takase (Tokyo Medical and Dental University)H-Index: 25
Last. Satoko Nakano (Oita University)H-Index: 8
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BACKGROUND Polymerase chain reaction (PCR) can be used to confirm or deny infectious ocular inflammation such as uveitis. The purpose of this article is to review the current practical use of PCR examination in ophthalmology, especially multiplex and broad-range PCR, and a novel PCR, termed Strip PCR. At first, in the Introduction, we show the development of the PCR examination in ophthalmology. We next show the clinical applications of multiplex PCR and broad-range PCR. These advances in PCR co...
Retinal ganglion cells (RGCs) are impaired in patients such as those with glaucoma and optic neuritis, resulting in permanent vision loss. To restore visual function, development of RGC transplantation therapy is now underway. Induced pluripotent stem cells (iPSCs) are an important source of RGCs for human allogeneic transplantation. We therefore analyzed the immunological characteristics of iPSC-derived RGCs (iPSC-RGCs) to evaluate the possibility of rejection after RGC transplantation. We firs...
2 CitationsSource
#1Kenichi MakabeH-Index: 6
#2Sunao SugitaH-Index: 40
Last. Masayo TakahashiH-Index: 46
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1 CitationsSource