R. Arancibia
Pontifical Catholic University of Chile
Internal medicinePathologyMolecular biologyMatrix (biology)Tumor necrosis factor alphaKinaseChemistryGrowth factorUrokinaseInflammationChitosanWound healingFibroblastPeriodontitisCTGFPlasminPeriodontal diseasePeriodontal tissueCancer researchConnective tissueViability assayBiochemistryDiabetes mellitusCell growthSignal transductionMedicineFibronectinBiologyCell biology
16Publications
8H-index
205Citations
Publications 16
Newest
#1Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
#2Claudia Metz (San Sebastián University)H-Index: 11
Last. Andrea Soza (UC: Pontifical Catholic University of Chile)H-Index: 18
view all 11 authors...
BACKGROUND AND OBJECTIVE During cyclosporine-induced gingival overgrowth, the homeostatic balance of gingival connective tissue is disrupted leading to fibrosis. Galectins are glycan-binding proteins that can modulate a variety of cellular processes including fibrosis in several organs. Here, we study the role of galectin-8 (Gal-8) in the response of gingival connective tissue cells to cyclosporine. METHODS We used human gingival fibroblasts and mouse NIH3T3 cells treated with recombinant Gal-8 ...
Source
#1L. Pavez (LMB: Laboratory of Molecular Biology)H-Index: 1
#2Nicolás Tobar (University of Chile)H-Index: 11
Last. Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
view all 10 authors...
Background and Objectives An important goal of periodontal therapy is the modulation of the inflammatory response. To this end, several pharmacological agents have been evaluated. Triclosan corresponds to an antibacterial and anti-inflammatory agent currently used in periodontal therapy. Chitosan is a natural polymer that may act as a drug delivery agent and exerts antibacterial and anti-inflammatory activities. Therefore, an association between both molecules might be useful to prevent inflamma...
4 CitationsSource
#1Ignacio N. Retamal (UC: Pontifical Catholic University of Chile)H-Index: 6
#2Romina Hernández (UC: Pontifical Catholic University of Chile)H-Index: 3
Last. Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
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Background and Objectives Methylglyoxal is a toxic product derived from glucose metabolism that plays a role in inflammation, diabetes and aging. In addition, the periodontal pathogen Tannerella forsythensis may also generate this compound. However, the effects of methylglyoxal on gingival cells are still poorly understood. In the present study, we have explored whether methylglyoxal or methylglyoxal-treated collagen may modulate cell viability, death and proliferation in gingival connective tis...
5 CitationsSource
#1A. Romero (UC: Pontifical Catholic University of Chile)H-Index: 2
#2Mónica Cáceres (UC: Pontifical Catholic University of Chile)H-Index: 16
Last. Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
view all 8 authors...
Background Granulation tissue remodeling and myofibroblastic differentiation are critically important events during wound healing. Tobacco smoking has a detrimental effect in gingival tissue repair. However, studies evaluating the effects of cigarette smoke on these events are lacking. Material and Methods We used gingival fibroblasts cultured within free-floating and restrained collagen gels to simulate the initial and final steps of the granulation tissue phase during tissue repair. Collagen g...
8 CitationsSource
#1Nicolás Tobar (University of Chile)H-Index: 11
#2Marcela Toyos (University of Chile)H-Index: 2
Last. Jorge Martínez (University of Chile)H-Index: 27
view all 7 authors...
Background Hard consistency, developed under the influence of tumor cell factors, is a characteristic feature of a breast tumor. Activation of resident fibroblasts leading to a myofibroblast phenotype is the principal feature that orchestrates this fibrotic process. The aim of this study was to assess the effects induced by TGF-β1, a growth factor abundantly present in tumor microenvironment, on the molecular mechanisms that mediate myofibroblastic differentiation of normal human mammary fibrobl...
21 CitationsSource
#1D. Silva (UC: Pontifical Catholic University of Chile)H-Index: 8
#2R. Arancibia (UC: Pontifical Catholic University of Chile)H-Index: 8
Last. Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
view all 8 authors...
Background and Objective Chitosan is a naturally derived polymer that may be applied in periodontal therapy for tissue-reconstruction purposes. Previous studies have shown that chitosan may stimulate tissue healing. However, reports exploring the cellular responses stimulated by chitosan are lacking. In the present study we analyzed whether chitosan may promote cell proliferation in primary cultures of human gingival fibroblasts. Material and Methods Chitosan particles were generated, and their ...
18 CitationsSource
#1R. Arancibia (UC: Pontifical Catholic University of Chile)H-Index: 8
#2Cristian Maturana (University of Chile)H-Index: 2
Last. Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
view all 8 authors...
Chitosan is a naturally derived polymer with antimicrobial and anti-inflammatory properties. However, studies evaluating the role of chitosan in the control of periodontal pathogens and the responses of fibroblasts to inflammatory stimuli are lacking. In the present study, we analyzed whether chitosan particles may inhibit the growth of periodontal pathogens and modulate the inflammatory response in human gingival fibroblasts. Chitosan particles were generated through ionic gelation. They inhibi...
47 CitationsSource
#1R. Arancibia (UC: Pontifical Catholic University of Chile)H-Index: 8
#2Alejandro Oyarzún (University of Chile)H-Index: 9
Last. Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
view all 6 authors...
Background: Fibroblasts play a critical role during wound healing and chronic inflammation through the synthesis and assembly of extracellular matrix (ECM) molecules. These responses may be modulated by soluble cytokines and growth factors present in tissues. In the present study, we evaluate whether transforming growth factor-β1 (TGF-β1) and tumor necrosis factor-α (TNF-α) modulate myofibroblastic differentiation and the production of ECM components.Methods: Primary cultures of human gingival f...
27 CitationsSource
#1D. Silva (UC: Pontifical Catholic University of Chile)H-Index: 8
#2Mónica Cáceres (UC: Pontifical Catholic University of Chile)H-Index: 16
Last. Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
view all 6 authors...
Silva D, Caceres M, Arancibia R, Martinez C, Martinez J, Smith P. Effects of cigarette smoke and nicotine on cell viability, migration and myofibroblastic differentiation. J Periodont Res 2012; 47: 599–607. © 2012 John Wiley & Sons A/S Background and Objective: Several studies have analysed the role of nicotine as a prominent agent affecting wound repair in smokers. However, tobacco smoke contains several components that may alter gingival wound healing. The present study aimed to analyse the ro...
33 CitationsSource
#1Patricio C. Smith (UC: Pontifical Catholic University of Chile)H-Index: 22
#2Ignacio N. Retamal (UC: Pontifical Catholic University of Chile)H-Index: 6
Last. Constanza Martínez (UC: Pontifical Catholic University of Chile)H-Index: 12
view all 7 authors...
Resumen Diabetes y enfermedad periodontal corresponden probablemente al mejor ejemplo de como una enfermedad sistemica puede tener un efecto en el territorio periodontal. Si bien esta asociacion ha sido extensamente estudiada, muchas de las asociaciones propuestas presentan contradicciones. En la presente revision de la literatura se analizan los siguientes topicos relevantes para la practica clinica en periodoncia e implantologia: i) Identificacion de enfermedad periodontal severa y su capacida...
2 CitationsSource