Mingtian Deng
Nanjing Agricultural University
GeneMessenger RNAMolecular biologyTranscriptomeAndrologyGene knockdownMaternal to zygotic transitionChemistryDNA methylationEmbryoGenomic imprintingSomatic cell nuclear transferHistone methylationBlastocystMethylationEpigeneticsGeneticsRNAReprogrammingBiologyCell biology
33Publications
10H-index
259Citations
Publications 33
Newest
#1Mingtian Deng (NAU: Nanjing Agricultural University)H-Index: 10
#2Baobao Chen (NAU: Nanjing Agricultural University)H-Index: 3
Last. Feng Wang (NAU: Nanjing Agricultural University)H-Index: 27
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Developmental arrest of somatic cell nuclear transfer (SCNT) embryos first occurs at zygotic/embryonic genome activation (ZGA/EGA), which is critical for preimplantation development. However, study on transcriptome of SCNT embryos during ZGA/EGA is limited. In the present study, we performed RNA-seq of the 8-cell SCNT embryos in goat and provide cross-species analysis of transcriptional activity of SCNT embryos during ZGA/EGA in mice, human, bovine, and goat. RNA-seq data revealed 3966 different...
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#1Kaiping Deng (NAU: Nanjing Agricultural University)H-Index: 6
#2Yixuan Fan (NAU: Nanjing Agricultural University)H-Index: 11
Last. Yanli Zhang (NAU: Nanjing Agricultural University)H-Index: 15
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Abstract null null N6-Methyladenosine (m6A) modification plays a critical role in mammalian development. However, the role of m6A in the skeletal muscle development remains largely unknown. Here, we report a global m6A modification pattern of goat skeletal muscle at two key development stages and identified that the m6A modification regulated the expression of the growth arrest and DNA damage response 45B (GADD45B) gene, which is involved in myogenic differentiation. We showed that GADD45B expre...
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#1Yongjie Wan (NAU: Nanjing Agricultural University)H-Index: 14
#2Dongxu Li (NAU: Nanjing Agricultural University)
Last. Feng Wang (NAU: Nanjing Agricultural University)H-Index: 27
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It has been reported that hypoxic environments were more suitable for the in vitro development of mammalian embryos, but the underlying mechanisms were still unclear. In the present study, RNA-seq was performed to compare 8-cell-stage and blastocyst-stage goat embryos under hypoxic and normoxic conditions; zygotes were checked at 72 and 168 h to 8-cell stage (L8C) and blastocyst stage (LM) in hypoxic conditions and 8-cell stage (H8C) and blastocyst stage (HM) in normoxic conditions. In the H8C a...
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#1Shi-Yu An (Nanjing Medical University)
#1An Shi Yu (NAU: Nanjing Agricultural University)
Last. Guomin Zhang (NAU: Nanjing Agricultural University)H-Index: 15
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Long non-coding RNAs (lncRNAs) regulate a complex array of fundamental biological processes, while its molecular regulatory mechanism in Leydig cells (LCs) remains unclear. In the present study, we established the lncRNA LOC102176306/miR-1197-3p/peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) regulatory network by bioinformatic prediction, and investigated its roles in goat LCs. We found that lncRNA LOC102176306 could efficiently bind to miR-1197-3p and regulate P...
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#1Xiaodan Li (NAU: Nanjing Agricultural University)H-Index: 2
#2Xiaolei Yao (NAU: Nanjing Agricultural University)H-Index: 8
Last. Feng Wang (NAU: Nanjing Agricultural University)H-Index: 27
view all 9 authors...
Abstract This study aimed to investigate the expression pattern of spermatogenesis associated protein 6 (SPATA6) in Hu sheep testis and to ascertain the effects of SPATA6 on sheep Leydig cells (LCs) function linked to spermatogenesis. In the present study, we detected a 1970 bp cDNA fragment of SPATA6 included a 1467 bp coding sequence which encoded 487 amino acids. Meanwhile, sheep SPATA6 shared 51.70%-97.41% amino acid sequences with its orthologs compared with other species. In addition, SPAT...
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#1Mingtian Deng (NAU: Nanjing Agricultural University)H-Index: 10
#2Yongjie Wan (NAU: Nanjing Agricultural University)H-Index: 14
Last. Feng Wang (NAU: Nanjing Agricultural University)H-Index: 27
view all 9 authors...
Abstract Long non-coding RNAs (lncRNAs) are involved in shaping chromosome conformation and regulation of preimplantation development. However, the role of lncRNA during somatic cell nuclear transfer (SCNT) reprogramming remains largely unknown. In the present study, we identified 114 upregulated lncRNAs in the 8-cell SCNT embryos as candidate key molecules involved in nuclear reprogramming in goat. We found that H3K4me3 was an epigenetic barrier in goat nuclear reprogramming and injection of Kd...
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#1Yu Cai (NAU: Nanjing Agricultural University)H-Index: 3
#2Ziyu Wang (NAU: Nanjing Agricultural University)H-Index: 10
Last. Feng Wang (NAU: Nanjing Agricultural University)H-Index: 27
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Abstract This study aimed to investigate the effects of dietary betaine supplementation on lipid metabolism, immunity, and testicular oxidative status in Hu sheep. Experimental sheep were fed with betaine-containing diets, a basal diet supplemented with 0 g/d (control group), 1 g/d (B1), and 3 g/d betaine (B2). The serum concentration of triglycerides was increased in Hu sheep receiving the diet supplemented with betaine. Regarding granulocytes, the ratio of monocytes, neutrophils and basophils ...
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#1Mingtian Deng (NAU: Nanjing Agricultural University)H-Index: 10
#2Guomin Zhang (NAU: Nanjing Agricultural University)H-Index: 15
Last. Yongjie Wan (NAU: Nanjing Agricultural University)H-Index: 14
view all 8 authors...
Abstract DNA methylation is a crucial element in the epigenetic regulation of mammalian embryonic development. However, the subtle changes in DNA methylation differ in species, and, little information is known regarding the dynamics of DNA methylation at the single-base resolution in goat. In the present study, we studied the DNA methylation dynamics during goat zygotic genome activation (ZGA) at global and single-base resolution using immunostaining and reduced representation bisulfite sequenci...
2 CitationsSource
#1Caifang Ren (Jiangsu University)
#2Kaiping Deng (NAU: Nanjing Agricultural University)H-Index: 6
Last. Feng Wang (NAU: Nanjing Agricultural University)H-Index: 27
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The more complex 3' UTR in higher organisms may have the function of increasing post-transcriptional gene regulation. Recent RNA sequencing technologies have provided us with the possibility to capture the complete 3' UTR landscape of different species and cells. However, no systematic analysis of sheep-related 3' UTR has been performed. Here, we conducted a detailed analysis of the 3' UTR with the primary goal of identifying intact 3' UTR landscapes in the sheep muscles of the three development...
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#1Yu Cai (NAU: Nanjing Agricultural University)H-Index: 3
#2Mingtian Deng (NAU: Nanjing Agricultural University)H-Index: 10
Last. Feng Wang (NAU: Nanjing Agricultural University)H-Index: 27
view all 9 authors...
Abstract Enhancer of zeste homolog 2 (EZH2) is a histone H3 lysine 27 (H3K27) methyltransferase that plays vital roles in mouse spermatogenesis. However, the expression pattern and role of EZH2 in goat spermatogonial stem cells (SSCs) is unknown. In the present study, we investigated EZH2 expression in the testis of postpubertal goats and its effect on the biological characteristics of goat SSCs. We found that EZH2 mRNA (P
1 CitationsSource