Teck Chuan Lim
Massachusetts Institute of Technology
Self-healing hydrogelsPolyproline helixBiophysicsBiomedical engineeringNeural stem cellCell migrationFluorescence correlation spectroscopyGlycosaminoglycanEndogenyStromal cellNeuroscienceRegeneration (biology)ChemistryImmunologyGelatinTissue engineeringDNAMedicineMicrofluidicsCell biology
Publications 14
#1Wanting NiuH-Index: 3
#2Teck Chuan LimH-Index: 9
Last. Myron SpectorH-Index: 67
view all 7 authors...
Bone marrow mesenchymal stem cells (bMSCs) are responsible in the repair of injured tissue through differentiation into multiple cell types and secretion of paracrine factors, and thus have a broad application profile in tissue engineering/regenerative medicine, especially for the musculoskeletal system. The lesion due to injury or disease may be a closed irregular-shaped cavity deep within tissue necessitating an injectable biomaterial permissive of host (endogenous) cell migration, proliferati...
#1Teck Chuan Lim (MIT: Massachusetts Institute of Technology)H-Index: 9
#2Emiri T. Mandeville (Harvard University)H-Index: 20
Last. Myron Spector (Brigham and Women's Hospital)H-Index: 67
view all 9 authors...
We assessed an injectable gelatin hydrogel containing epidermal growth factor (Gtn-EGF) as a therapy for intracerebral hemorrhage (ICH). ICH was induced in rats via collagenase injection into the striatum. Two weeks later, Gtn-EGF was injected into the cavitary lesion. The hydrogel filled ICH cavities without deforming brain tissue. Immunostaining demonstrated that neural precursor cells could migrate into the matrix, and some of these differentiated into neurons along with the appearance of ast...
6 CitationsSource
#1Shuting CaiH-Index: 2
#2Daniel H. LukamtoH-Index: 2
Last. Song-Gil LeeH-Index: 4
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A significant barrier to harnessing the power of cell-surface glycosaminoglycans (GAGs) to modulate glial cell-line-derived neurotrophic factor (GDNF) signaling is the difficulty in accessing key GAG structures involved. Here, we report tailored GDNF signaling using synthetic polyproline-based GAG mimetics (PGMs). PGMs deliver the much needed proactive programmability for GDNF recognition and effectively modulate GDNF-mediated neuronal processes in a cellular context.
#1Teck Chuan LimH-Index: 9
#2Shuting CaiH-Index: 2
Last. Song-Gil LeeH-Index: 4
view all 9 authors...
Controlling glycosaminoglycan (GAG) activity to exploit its immense potential in biology ultimately requires facile manipulation of sulfation patterns associated with GAGs. However, satisfying this requirement in full remains challenging, given that synthesis of GAGs is technically arduous while convenient GAG mimetics often produce sulfation patterns that are uncharacteristic of GAGs. To overcome this, we develop saccharide-free polyproline-based GAG mimetics (PGMs) that can be facilely assembl...
5 CitationsSource
The health of the central nervous system (CNS) does not only rely on the state of the neural cells but also on how various extracellular components organize cellular behaviors into proper tissue functions. Biomaterials have been valuable in restoring or augmenting the roles of extracellular components in the CNS in the event of injury and disease. In this review, we highlight how biomaterials have been enabling tools in important therapeutic strategies involving cell transplantation and drug/pro...
18 CitationsSource
#1Myron Spector (Brigham and Women's Hospital)H-Index: 67
#2Teck Chuan Lim (MIT: Massachusetts Institute of Technology)H-Index: 9
We are experiencing a new wave of injectable therapeutics (namely/injectable biomaterials) to complement injectable drugs and injectable biologics, and to serve as the basis for injectable combinatorial therapeutics. Injectable biomaterials contribute to the treatment of the fluid-filled defects which often result from disease and injury, by providing the missing physical framework (i.e. the stroma). However, while injectable matrices may be necessary for the successful treatment of certain lesi...
20 CitationsSource
#1Teck Chuan LimH-Index: 9
#1Teck Chuan Lim (MIT: Massachusetts Institute of Technology)H-Index: 9
#2Swetha Rokkappanavar (Brigham and Women's Hospital)H-Index: 1
Last. Myron Spector (MIT: Massachusetts Institute of Technology)H-Index: 67
view all 6 authors...
Without chemotactic cues and structural support, cavitary brain lesions typically fail to recruit endogenous neural progenitor cells (NPCs). Toward resolving this, we engineered multifunctional biomaterials comprising injectable gelatin-hydroxyphenylpropionic acid (Gtn-HPA) hydrogels and dextran sulfate/chitosan polyelectrolyte complex nanoparticles (PCNs) that delivered stromal cell-derived factor-1α (SDF-1α). Over 7 d of interface with in vitro tissue simulant containing adult rat hippocampal ...
42 CitationsSource
#1Wei Seong Toh (Brigham and Women's Hospital)H-Index: 31
#2Teck Chuan Lim (MIT: Massachusetts Institute of Technology)H-Index: 9
Last. Myron Spector (MIT: Massachusetts Institute of Technology)H-Index: 67
view all 4 authors...
Abstract An injectable and biodegradable hydrogel system comprising hyaluronic acid-tyramine (HA-Tyr) conjugates can safely undergo covalent cross-linking in vivo by the addition of small amounts of peroxidase and hydrogen peroxide (H 2 O 2 ), with the independent tuning of the gelation rate and degree of cross-linking. Such hydrogel networks with tunable mechanical and degradation properties may provide the additional level of control needed to enhance chondrogenesis and overall cartilage tissu...
187 CitationsSource
#1Teck Chuan Lim (MIT: Massachusetts Institute of Technology)H-Index: 9
#2Wei Seong Toh (Brigham and Women's Hospital)H-Index: 31
Last. Myron Spector (MIT: Massachusetts Institute of Technology)H-Index: 67
view all 5 authors...
Abstract Transplanted or endogenous neural stem cells often lack appropriate matrix in cavitary lesions in the central nervous system. In this study, gelatin-hydroxyphenylpropionic acid (Gtn-HPA), which could be enzymatically crosslinked with independent tuning of crosslinking degree and gelation rate, was explored as an injectable hydrogel for adult neural stem cells (aNSCs). The storage modulus of Gtn-HPA could be tuned (449–1717 Pa) to approximate adult brain tissue. Gtn-HPA was cytocompatibl...
69 CitationsSource