Pierre Comizzoli
Smithsonian Conservation Biology Institute
AnatomyInternal medicineEndocrinologyAndrologyEndangered speciesCryopreservationChemistryEcologyEmbryoOocyteFertility preservationVitrificationSpermWildlifeGerminal vesicleReproductive technologyFolliculogenesisBiologyHuman fertilizationZoologyCell biology
163Publications
22H-index
1,751Citations
Publications 161
Newest
#1Séverine Le Gac (MESA+ Institute for Nanotechnology)H-Index: 25
#2Marcia de Almeida Monteiro Melo Ferraz (LMU: Ludwig Maximilian University of Munich)H-Index: 6
Last. Pierre Comizzoli (SCBI: Smithsonian Conservation Biology Institute)H-Index: 22
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Conservation breeding and assisted reproductive technologies (ARTs) are invaluable tools to save wild animal species that are on the brink of extinction. Microfluidic devices recently developed for human or domestic animal reproductive medicine could significantly help to increase knowledge about fertility and contribute to the success of ART in wildlife. Some of these microfluidic tools could be applied to wild species, but dedicated efforts will be necessary to meet specific needs in animal co...
2 CitationsSource
#1P. Thuwanut (Chula: Chulalongkorn University)H-Index: 11
#2Pierre Comizzoli (SCBI: Smithsonian Conservation Biology Institute)H-Index: 22
Last. Porntip Sirayapiwat (Chula: Chulalongkorn University)H-Index: 4
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OBJECTIVE Using domestic cats as a biomedical research model for fertility preservation, the present study aimed to characterize the influences of ovarian tissue encapsulation in biodegradable hydrogel matrix (fibrinogen/thrombin) on resilience to cryopreservation, and static versus non-static culture systems following ovarian tissue encapsulation and cryopreservation on follicle quality. METHODS In experiment I, ovarian tissues (n=21 animals; 567 ovarian fragments) were assigned to controls or ...
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#1Pierre Comizzoli (SCBI: Smithsonian Conservation Biology Institute)H-Index: 22
#2Mary Ann Ottinger (UH: University of Houston)H-Index: 47
Similar to humans and laboratory animals, reproductive aging is observed in wild species - from small invertebrates to large mammals. Aging issues are also prevalent in rare and endangered species under human care as their life expectancy is longer than in the wild. The objectives of this review are to (1) present conserved as well as distinctive traits of reproductive aging in different wild animal species (2) highlight the value of comparative studies to address aging issues in conservation br...
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#1Tricia Rowlison (SCBI: Smithsonian Conservation Biology Institute)H-Index: 2
#2Mary Ann Ottinger (UH: University of Houston)H-Index: 47
Last. Pierre Comizzoli (SCBI: Smithsonian Conservation Biology Institute)H-Index: 22
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PURPOSE Extracellular vesicles (EVs) secreted by the epididymal epithelium transfer key factors to maturing spermatozoa. Using an in vitro system previously developed in our laboratory, the objective was to (1) characterize the impact of EV exposure on the fertilizing ability and developmental potential of immature sperm cells from the caput epididymidis and (2) examine the benefit of EV exposure to restore vitality of mature spermatozoa from the cauda epididymidis after freezing-thawing. METHOD...
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#1Bin Jiang (UMD: University of Maryland, College Park)H-Index: 10
#2Weijie Li (UMD: University of Maryland, College Park)
Last. Xiaoming He (UMD: University of Maryland, College Park)H-Index: 44
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Human induced pluripotent stem cells (hiPSCs) possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use. However, contemporary methods for hiPSC cryopreservation are associated with both limited cell survival and high concentration of toxic cryoprotectants and/or serum. The latter may cause spontaneous differentiation and/or introduce xenogeneic factors, which may compromise the quality of hiPSCs. H...
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#1E E Paulson (SCBI: Smithsonian Conservation Biology Institute)
#2Pierre Comizzoli (SCBI: Smithsonian Conservation Biology Institute)H-Index: 22
Endometrial receptivity and embryo implantation processes are a major point of pregnancy failure in many mammalian species, including humans. Although reproductive biology in many carnivore species remains enigmatic, the few that have been studied so far are invaluable comparative models. The goals of this review are to (1) summarize current data on the mechanisms involved in uterine receptivity and embryo implantation in carnivores including commonalities and differences with other mammalian sp...
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Abstract The objective of the study was to evaluate the effects of different cryopreservation techniques including glycerol-based cryoprotectant combinations on the structure and viability of testicular tissues from adult collared peccaries. Tissue biopsies (3.0 mm³) from 5 different individuals were allocated to 10 different groups: fresh control; slow freezing (SF), conventional vitrification (CV), or solid-surface vitrification (SSV); each of them using three different combinations of cryopro...
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#1Pierre Comizzoli (SCBI: Smithsonian Conservation Biology Institute)H-Index: 22
The success of a pregnancy and the birth of a heathy baby following embryo transfer are conditioned by many factors, including embryo quality and the uterine environment. While we keep looking for more indicators of embryo quality, it also is critical to understand what constitutes a favorable uterine environment leading to a successful pregnancy and birth. This issue of JARG offers new insights on both components-so called by some "the seed and the soil"-and also highlights the critical interac...
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The binturong is a medium size carnivore belonging to the Viverrid family that lives in dense forests of South-East Asia. In addition to the protection of this vulnerable species in its natural habitat (in situ), conservation breeding efforts (ex situ) aim at maintaining a good genetic diversity while increasing the number of individuals to reinforce wild populations. Both approaches require a solid understanding of binturong's basic biology. However, there is still a lack of precise information...
1 CitationsSource
Maintaining a stable dry state is critical for long-term preservation of live biomaterials at suprazero temperatures. The objective of the study was to characterize the effect of moisture content on DNA integrity within the germinal vesicle (GV) of feline oocytes following dehydration and storage at 22–24 °C. Using microwave-assisted drying, conditions that led to a predictable and stable moisture content in trehalose solutions were determined. To explore moisture content stability during storag...
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