Josefin Ahnström
Imperial College London
Binding siteSignal peptideProthrombinaseInternal medicineEndocrinologyMolecular biologyChemistryTissue factorCofactorLipocalinFactor VProtein STissue factor pathway inhibitorThrombinKunitz domainCholesterolAPOMProtein CBiochemistryApolipoprotein BMedicineBiologyCell biology
43Publications
16H-index
1,244Citations
Publications 43
Newest
#1Josefin Ahnström (Imperial College London)H-Index: 16
Source
#1Josefin Ahnström (Imperial College London)H-Index: 16
#2Gary E. Gilbert (VA Boston Healthcare System)H-Index: 28
Source
#1Katherine J. Kearney (University of Leeds)H-Index: 3
#2Juliet Butler (University of Leeds)H-Index: 1
Last. Helen Philippou (University of Leeds)H-Index: 30
view all 13 authors...
Kallikrein (PKa), generated by activation of its precursor prekallikrein (PK), plays a role in the contact activation phase of coagulation and functions in the kallikrein-kinin system to generate bradykinin. The general dogma has been that the contribution of PKa to the coagulation cascade is dependent on its action on FXII. Recently this dogma has been challenged by studies in human plasma showing thrombin generation due to PKa activity on FIX and also by murine studies showing formation of FIX...
3 CitationsSource
#1Salvatore Santamaria (Imperial College London)H-Index: 14
#2Doretta Cuffaro (UniPi: University of Pisa)H-Index: 5
Last. Josefin Ahnström (Imperial College London)H-Index: 16
view all 10 authors...
ADAMTS-5 is a major protease involved in the turnover of proteoglycans such as aggrecan and versican. Dysregulated aggrecanase activity of ADAMTS-5 has been directly linked to the etiology of osteoarthritis (OA). For this reason, ADAMTS-5 is a pharmaceutical target for the treatment of OA. ADAMTS-5 shares high structural and functional similarities with ADAMTS-4, which makes the design of selective inhibitors particularly challenging. Here we exploited the ADAMTS-5 binding capacity of β-N-acetyl...
4 CitationsSource
#2Josefin Ahnström (Imperial College London)H-Index: 16
Last. Richard A. Marlar (UNM: University of New Mexico)H-Index: 4
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Hereditary deficiencies of protein S (PS) increase the risk of thrombosis. However, assessing the plasma levels of PS is complicated by its manifold physiological interactions, while the large inter-individual variability makes it problematic to establish reliable cut-off values. PS has multiple physiological functions, with only two appearing to have significant anticoagulant properties: the activated protein C (APC) and tissue factor pathway inhibitor alpha (TFPIα) cofactor activities. Current...
Source
#1Magdalena Gierula (Imperial College London)H-Index: 8
#2Josefin Ahnström (Imperial College London)H-Index: 16
Protein S is a critical regulator of coagulation that functions as a cofactor for the activated protein C (APC) and tissue factor pathway inhibitor (TFPI) pathways. It also has direct anticoagulant functions, inhibiting the intrinsic tenase and prothrombinase complexes. Through these functions, protein S regulates coagulation during both its initiation and its propagation phases. The importance of protein S in haemostatic regulation is apparent from the strong association between protein S defic...
3 CitationsSource
#1Plinio Ferreira (University of Reading)H-Index: 3
#2E Bozbas (University of Reading)H-Index: 1
Last. Parveen Yaqoob (University of Reading)H-Index: 68
view all 10 authors...
Platelet-derived extracellular vesicles (PDEVs) are the most abundant amongst all types of EVs in the circulation. However, the mechanisms leading to PDEVs release, their role in coagulation and phenotypic composition are poorly understood. PDEVs from washed platelets were generated using different stimuli and were characterised using nanoparticle tracking analysis. Procoagulant properties were evaluated by fluorescence flow cytometry and calibrated automated thrombography. EVs from plasma were ...
1 CitationsSource
#1Josefin Ahnström (Imperial College London)H-Index: 16
#2Magdalena Gierula (Imperial College London)H-Index: 8
Last. David A. Lane (Imperial College London)H-Index: 11
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BACKGROUND: Activated coagulation factor X (FXa) is the serine protease component of prothrombinase, the physiological activator of prothrombin. Factor X Nottingham (A404T) and Taunton (R405G) are two naturally occurring mutations, identified in families with a bleeding phenotype. OBJECTIVE: To characterize these FX variants functionally. METHODS: The activity and inhibition of recombinant FX variants were quantified in plasma-based and pure component assays. RESULTS: The prothrombin times in FX...
1 CitationsSource
#1Claire Peghaire (NIH: National Institutes of Health)H-Index: 9
#2Neil Dufton (NIH: National Institutes of Health)H-Index: 13
Last. Anna M. Randi (NIH: National Institutes of Health)H-Index: 30
view all 14 authors...
Endothelial cells actively maintain an anti-thrombotic environment; loss of this protective function may lead to thrombosis and systemic coagulopathy. The transcription factor ERG is essential to maintain endothelial homeostasis. Here, we show that inducible endothelial ERG deletion (ErgiEC-KO) in mice is associated with spontaneous thrombosis, hemorrhages and systemic coagulopathy. We find that ERG drives transcription of the anticoagulant thrombomodulin (TM), as shown by reporter assays and ch...
9 CitationsSource
1 CitationsSource