Kevin D. Neibert
McGill University
CytotoxicityNeuriteAnalytical chemistryDocking (molecular)Quantum dotBiophysicsNanotoxicologyDrug carrierTFEBConfocalInternalizationDynamic light scatteringTransporterCellNanomedicineNanoparticleCellular adaptationComposite materialNeurotrophic factorsFluorescence microscopeNeuroscienceHEK 293 cellsOrganic chemistryMolecular modelCovalent bondEnzymeNanocarriersAqueous solutionLiposomeChemistryCell typeMaterials scienceIn vitroMacromoleculeIn vivoCombinatorial chemistryIn silicoOxidative stressEthylene glycolGlutaraldehydeNitric oxide synthaseAntioxidantSwellingDrug deliveryMicrogliaLysosomeLesionBODIPYEndocytosisCytoprotective AgentFibroblast migrationToxicitySilver nanoparticlePolymersomeCentral nervous systemViability assayGlutathionePEG ratioFlow cytometryBiochemistryDendrimerFluorescence-lifetime imaging microscopyAmphiphileClick chemistryStereochemistryAbsorption (chemistry)IntracellularPrecipitation (chemistry)LigandZeta potentialBiologyFluorescenceNeurotrophinOptical imagingCell biologyMultiangle light scattering
Publications 9
#1Alexandre Moquin (McGill University)H-Index: 11
#2Jeff Ji (McGill University)H-Index: 5
Last. Dusica Maysinger (McGill University)H-Index: 65
view all 5 authors...
Polymersomes are attractive nanocarriers for hydrophilic and lipophilic drugs; they are more stable than liposomes, tunable, and relatively easy to prepare. The copolymer composition and molar mass are critical features that determine the physicochemical properties of the polymersomes including the rate of drug release. We used the triblock-copolymer, poly(2-methyl-2-oxazoline)-block-poly-(dimethysiloxane)-block-poly(2-methyl-2-oxazoline) (PMOXA–PDMS–PMOXA), to form amphipathic polymersomes capa...
13 CitationsSource
#1Alexandre Moquin (McGill University)H-Index: 11
#2Kevin D. Neibert (McGill University)H-Index: 8
Last. Françoise M. Winnik (UdeM: Université de Montréal)H-Index: 76
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Abstract The molecular composition of the biological environment of nanoparticles influences their physical properties and changes their pristine physicochemical identity. In order to understand, or predict, the interactions of cells with specific nanoparticles, it is critical to know their size, shape, and agglomeration state not only in their nascent state but also in biological media. Here, we use asymmetrical flow field-flow fractionation (AF4) with on-line multiangle light scattering (MALS)...
16 CitationsSource
#1Angela O. Choi (McGill University)H-Index: 8
#2Kevin D. Neibert (McGill University)H-Index: 8
Last. Dusica Maysinger (McGill University)H-Index: 65
view all 3 authors...
: Quantum dots (QDs) have been used for optical imaging of neural cells in vitro and in vivo. This chapter lists the basic materials, instrumentation and step-by-step procedures to image live microglia cells and to show the functional and biochemical changes in microglia exposed to QDs. Details are also provided for the real-time imaging of cerebral ischemic lesions in animals and for the assessment of lesion reduction after therapeutic interventions. Microglia are brain cells which detect, inte...
3 CitationsSource
#1Kevin D. Neibert (McGill University)H-Index: 8
#2Varin Gosein (McGill University)H-Index: 4
Last. Ashok K. KakkarH-Index: 38
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These studies explore the relationship between the inhibitory actions of low generation dendrimers in stimulated microglia and dendrimer–enzyme interactions using in silico molecular modeling. Low generation (DG0 and DG1) dendrimers with acetylene and hydroxyl terminal groups were tested for their anti-inflammatory activity in microglia stimulated by lipopolysaccharides (LPS), and the results were compared with those from the established anti-inflammatory agents, ibuprofen and celecoxib. We hypo...
27 CitationsSource
#1Kevin D. Neibert (McGill University)H-Index: 8
#2Venkateshwarlu Gopishetty (Clarkson University)H-Index: 9
Last. Dusica Maysinger (McGill University)H-Index: 65
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Abstract The objective of this study is to provide a novel synthetic approach for the manufacture of wound-healing materials using covalently cross-linked alginate fibers loaded with silver nanoparticles. Alginate fibers are prepared by wet-spinning in a CaCl(2) precipitation bath. Using this same approach, calcium cross-links in alginate fibers are replaced by chemical cross-links that involve hydroxyl groups for subsequent cross-linking by glutaraldehyde. The cross-linked fibers become highly ...
50 CitationsSource
#1Kevin D. Neibert (McGill University)H-Index: 8
#2Dusica Maysinger (McGill University)H-Index: 65
AbstractCellular adaptation is the dynamic response of a cell to adverse changes in its intra/extra cellular environment. The aims of this study were to investigate the role of: (i) the glutathione antioxidant system, and (ii) the transcription factor EB (TFEB), a newly revealed master regulator of lysosome biogenesis, in cellular adaptation to nanoparticle-induced oxidative stress. Intracellular concentrations of glutathione species and activation of TFEB were assessed in rat pheochromocytoma (...
40 CitationsSource
#1Noura Al-Hajaj (McGill University)H-Index: 4
#2Alexandre MoquinH-Index: 11
Last. Dusica Maysinger (McGill University)H-Index: 65
view all 6 authors...
In order to better understand nanoparticle uptake and elimination mechanisms, we designed a controlled set of small, highly fluorescent quantum dots (QDs) with nearly identical hydrodynamic size (8–10 nm) but with varied short ligand surface functionalization. The properties of functionalized QDs and their modes of uptake and elimination were investigated systematically by asymmetrical flow field–flow fractionation (AF4), confocal fluorescence microscopy, flow cytometry (FACS), and flame atomic ...
70 CitationsSource
#1Anjali SharmaH-Index: 14
#2Kevin D. NeibertH-Index: 8
Last. Ashok K. KakkarH-Index: 38
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Bi- and trifunctional dendrimer and miktoarm nanocarriers. containing a combination of covalently linked model drug (α-lipoic acid), fluorescent dye (BODIPY), and poly(ethylene glycol) (PEG), are synthesized using a core with orthogonal functional groups, on which Huisgen alkyne―azide "click" reactions are performed in sequence. These carriers are internalized into the cells where they reduce H 2 O 2 induced reactive oxygen species formation. In addition, miktoarm nanocarriers conjugated with α-...
23 CitationsSource
#1Manasi P Jain (McGill University)H-Index: 4
#2Angela O. ChoiH-Index: 8
Last. Dusica Maysinger (McGill University)H-Index: 65
view all 4 authors...
Aim: Toxicity of nanoparticles developed for biomedical applications is extensively debated as no uniform guidelines are available for studying nanomaterial safety, resulting in conflicting data obtained from different cell types. This study demonstrates the varied toxicity of a selected type of nanoparticle, cadmium telluride quantum dots (QDs), in three increasingly complex cell models of the peripheral nervous system. Materials & methods: QD-induced cytotoxicity was assessed via cell viabilit...
19 CitationsSource