Dominique Desplancq
Centre national de la recherche scientifique
Binding siteTransduction (genetics)Fusion proteinGreen fluorescent proteinDNA damageAntigenMolecular biologyAntibodyEndogenyDNA gyraseChemistryPhage displayElectroporationCytoplasmEpitopeIn vitroIntrabodyAnabaenaPeptideNADPH oxidaseAlanine scanningTarget proteinPhosphorylated Histone H2AXHistone H2AXNuclear proteinTranscription (biology)Proliferating cell nuclear antigenRecombinant DNAProtein structureTransfectionMonoclonal antibodyEscherichia coliDNABiochemistryRNA polymerase IINuclear magnetic resonance spectroscopyStereochemistryDNA replicationTranscription factorBiologyCell biologyCancer cellCytosol
23Publications
12H-index
460Citations
Publications 23
Newest
#1Eric MoeglinH-Index: 2
#2Dominique DesplancqH-Index: 12
view all 14 authors...
Histone H2AX phosphorylated at serine 139 (γ-H2AX) is a hallmark of DNA damage, signaling the presence of DNA double-strand breaks and global replication stress in mammalian cells. While γ-H2AX can be visualized with antibodies in fixed cells, its detection in living cells was so far not possible. Here, we used immune libraries and phage display to isolate nanobodies that specifically bind to γ-H2AX. We solved the crystal structure of the most soluble nanobody in complex with the phosphopeptide ...
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#1Delphine Ohayon (French Institute of Health and Medical Research)H-Index: 5
#1Delphine Ohayon (French Institute of Health and Medical Research)H-Index: 1
Last. Véronique Witko-Sarsat (French Institute of Health and Medical Research)H-Index: 47
view all 24 authors...
Neutrophils produce high levels of reactive oxygen species (ROS) by NADPH oxidase that are crucial for host defense but can lead to tissue injury when produced in excess. We previously described that proliferating cell nuclear antigen (PCNA), a nuclear scaffolding protein pivotal in DNA synthesis, controls neutrophil survival through its cytosolic association with procaspases. We herein showed that PCNA associated with p47phox, a key subunit of NADPH oxidase, and that this association regulated ...
7 CitationsSource
#1Eric Moeglin (UDS: University of Strasbourg)H-Index: 2
#2Dominique Desplancq (UDS: University of Strasbourg)H-Index: 12
Last. Etienne Weiss (UDS: University of Strasbourg)H-Index: 25
view all 10 authors...
Phosphorylated histone H2AX (γ-H2AX), a central player in the DNA damage response (DDR), serves as a biomarker of DNA double-strand break repair. Although DNA damage is generally visualized by the formation of γ-H2AX foci in injured nuclei, it is unclear whether the widespread uniform nuclear γ-H2AX (called pan-nuclear) pattern occurring upon intense replication stress (RS) is linked to DDR. Using a novel monoclonal antibody that binds exclusively to the phosphorylated C-terminus of H2AX, we dem...
28 CitationsSource
#1Sascha ConicH-Index: 5
#2Dominique Desplancq (UDS: University of Strasbourg)H-Index: 12
Last. Laszlo ToraH-Index: 81
view all 6 authors...
: In this chapter, we describe an antibody electroporation-based imaging approach that allows for precise imaging and quantification of endogenous transcription factor (i.e., RNA Polymerase II) distributions in single cells using 3D structured illumination microscopy (3D-SIM). The labeling is achieved by the efficient and harmless delivery of fluorescent dye-conjugated antibodies into living cells and the specific binding of these antibodies to the targeted factors. Our step-by-step protocol des...
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#1Sascha ConicH-Index: 5
#2Dominique DesplancqH-Index: 12
Last. Etienne WeissH-Index: 25
view all 4 authors...
: The spatiotemporal localization of different intracellular factors in real-time and their detection in live cells are important parameters to understand dynamic protein-based processes. Therefore, there is a demand to perform live-cell imaging and to measure endogenous protein dynamics in single cells. However, fluorescent labeling of endogenous protein in living cells without overexpression of fusion proteins or genetic tagging has not been routinely possible. Here we describe a versatile ant...
2 CitationsSource
#1Dominique Desplancq (UDS: University of Strasbourg)H-Index: 12
#2Nadja GroysbeckH-Index: 2
Last. Guy Zuber (UDS: University of Strasbourg)H-Index: 26
view all 15 authors...
For biological and medical applications, a definable nanomaterial and knowledge on its fate after administration are highly recommended if not mandatory. Here, we synthesized a water-soluble gold nanocluster by sodium borohydride reduction of chloroauric acid in the presence of 5,5′-dithio-bis(2-nitrobenzoic acid). The resulting gold nanocluster displayed the physical characteristics of a cluster containing an inner core of about 100 gold atoms that was surrounded by an organic monolayer made of...
4 CitationsSource
#1Sascha ConicH-Index: 5
#2Dominique DesplancqH-Index: 12
Last. Laszlo ToraH-Index: 81
view all 13 authors...
Fluorescent labeling of endogenous proteins for live-cell imaging without exogenous expression of tagged proteins or genetic manipulations has not been routinely possible. We describe a simple versatile antibody-based imaging approach (VANIMA) for the precise localization and tracking of endogenous nuclear factors. Our protocol can be implemented in every laboratory allowing the efficient and nonharmful delivery of organic dye-conjugated antibodies, or antibody fragments, into different metazoan...
22 CitationsSource
#1Dominique Desplancq (UDS: University of Strasbourg)H-Index: 12
#2Guillaume Freund (UDS: University of Strasbourg)H-Index: 4
Last. Etienne Weiss (UDS: University of Strasbourg)H-Index: 25
view all 13 authors...
Although chemical inhibition of the DNA damage response (DDR) in cancer cells triggers cell death, it is not clear if the fork blockade achieved with inhibitors that neutralise proteins of the replisome is sufficient on its own to overcome the DDR. Monoclonal antibodies to PCNA, which block the DNA elongation process in vitro, have been developed. When these antibodies were transduced into cancer cells, they are able to inhibit the incorporation of nucleoside analogues. When co-delivered with an...
16 CitationsSource
#1Viktoriia Y. Postupalenko (UDS: University of Strasbourg)H-Index: 11
#2Dominique Desplancq ('ENS Paris': École Normale Supérieure)H-Index: 12
Last. Guy Zuber (UDS: University of Strasbourg)H-Index: 26
view all 10 authors...
Recombinant proteins with cytosolic or nuclear activities are emerging as tools for interfering with cellular functions. Because such tools rely on vehicles for crossing the plasma membrane we developed a protein delivery system consisting in the assembly of pyridylthiourea-grafted polyethylenimine (πPEI) with affinity-purified His-tagged proteins pre-organized onto a nickel-immobilized polymeric guide. The guide was prepared by functionalization of an ornithine polymer with nitrilotriacetic aci...
45 CitationsSource