Raffaella Fazzina
University of Bologna
LuciferaseNeural stem cellCellular differentiationMolecular biologyBioluminescence imagingMesenchymal stem cellImmunologyPlatelet lysateSOX2Myeloid leukemiaErythropoiesisBone marrowStem cellCell cycleCancer researchReprogrammingCell cultureBiologyCell biologyEctopic expression
Publications 13
#1Paola IudiconeH-Index: 10
#2Daniela FioravantiH-Index: 7
Last. Luca Pierelli (Sapienza University of Rome)H-Index: 34
view all 8 authors...
Abstract CIK cells are a subset of effector lymphocytes endowed with a non-MHC restricted anti-tumor activity making them an appealing and promising cell population for adoptive immunotherapy. CIK are usually generated ex-vivo by initial priming with Interferon-γ (IFN-γ) and monoclonal antibody against CD3 (anti-CD3), followed by culture in medium containing Interleukin-2 (IL-2). Interleukin-15 (IL-15) shares with IL-2 similar biological functions and recently it has been reported to induce CIK ...
5 CitationsSource
#1Raffaella FazzinaH-Index: 8
#2Paola IudiconeH-Index: 10
Last. Luca Pierelli (Sapienza University of Rome)H-Index: 34
view all 7 authors...
Background Mesenchymal stromal cells (MSCs) have been largely investigated, in the past decade, as potential therapeutic strategies for various acute and chronic pathological conditions. MSCs isolated from different sources, such as bone marrow (BM), umbilical cord tissue (UCT) and adipose tissue (AT), share many biological features, although they may show some differences on cumulative yield, proliferative ability and differentiation potential. The standardization of MSCs growth and their funct...
21 CitationsSource
#1Raffaella FazzinaH-Index: 8
#2Paola IudiconeH-Index: 10
Last. Luca PierelliH-Index: 34
view all 9 authors...
Alternatives to the use of fetal bovine serum (FBS) have been investigated to ensure xeno-free growth condition. In this study we evaluated the efficacy of human platelet lysate (PL) as a substitute of FBS for the in vitro culture of some human cell lines. PL was obtained by pools of pathogen inactivated human donor platelet (PLT) concentrates. Human leukemia cell lines (KG-1, K562, JURKAT, HL-60) and epithelial tumor cell lines (HeLa and MCF-7) were cultured with either FBS or PL. Changes in ce...
17 CitationsSource
#1Raffaella FazzinaH-Index: 8
#2Andrea Mariotti (CUA: The Catholic University of America)H-Index: 14
Last. Giuseppina Bonanno (CUA: The Catholic University of America)H-Index: 33
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BACKGROUND Mesenchymal stromal cells (MSCs) isolated from human umbilical cord tissue (UCT) can be considered the perfect candidates for cell-based therapies and regenerative medicine. UCT-derived MSCs can be cryogenically stored in cell banks and expanded as needed for therapeutic uses. STUDY DESIGN AND METHODS We developed a new method for UCT-MSC isolation, cryopreservation, and expansion, following all criteria required by a stem cell bank. UCT-MSCs were isolated either by manual dissociatio...
8 CitationsSource
#1Raffaella Fazzina (UNIBO: University of Bologna)H-Index: 8
#2Lorenza Lombardini (UNIBO: University of Bologna)H-Index: 2
Last. Roberto Tonelli (UNIBO: University of Bologna)H-Index: 9
view all 7 authors...
: Chromosomal translocations involving the MLL gene on 11q23 present frequent abnormalities in pediatric, adult and therapy-related acute leukemias, and are generally associated with aggressive disease and poor prognosis. Here, we report bioluminescent acute leukemia xenograft mouse models of the most frequent and aggressive MLL-related acute leukemias (infant and adult MLL-AF9, MLL-ENL, MLL-AF4). Four acute leukemia cell lines carrying MLL-related translocations were stably transduced with a fi...
8 CitationsSource
#2Maria C. Marchetto (Salk Institute for Biological Studies)H-Index: 44
Last. Juan Carlos Izpisua Belmonte (Salk Institute for Biological Studies)H-Index: 90
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The finding that certain somatic cells can be directly converted into cells of other lineages by the delivery of specific sets of transcription factors paves the way to novel therapeutic applications. Here we show that human cord blood (CB) CD133+ cells lose their hematopoietic signature and are converted into CB-induced neuronal-like cells (CB-iNCs) by the ectopic expression of the transcription factor Sox2, a process that is further augmented by the combination of Sox2 and c-Myc. Gene-expressi...
54 CitationsSource
Last. Juan Carlos Izpisua (Salk Institute for Biological Studies)H-Index: 4
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Abstract 2350 The finding that the epigenome of differentiated cells can be reset to a pluripotent state indicates that any somatic cell may potentially change or reverse its already established developmental identity through the delivery of appropriate instructive signals. Here we show the possibility of generating mature and functional neurons from Cord Blood (CB) cells without reprogramming into a pluripotent state. In particular, we demonstrate that the ectopic expression of two transcriptio...
1 CitationsSource
#1Laura Mezzanotte (UNIBO: University of Bologna)H-Index: 14
#2Raffaella Fazzina (UNIBO: University of Bologna)H-Index: 8
Last. Aldo Roda (UNIBO: University of Bologna)H-Index: 57
view all 7 authors...
Purpose Conventional in vivo bioluminescence imaging using wild-type green-emitting luciferase is limited by absorption and scattering of the bioluminescent signal through tissues. Imaging methods using a red-shifted thermostable luciferase from Photinus pyralis were optimized to improve the sensitivity and image resolution. In vivo bioluminescence imaging performance of red- and green-emitting luciferases were compared in two different xenograft mouse models for cancer.
26 CitationsSource
By expressing EVI1 in murine bone marrow (BM), we previously described a myelodysplastic syndrome (MDS) model characterized by pancytopenia, dysmegakaryopoiesis, dyserythropoiesis, and BM failure. The mice invariably died 11–14 months after BM transplantation (BMT). Here, we show that a double point mutant EVI1-(1+6Mut), unable to bind Gata1, abrogates the onset of MDS in the mouse and re-establishes normal megakaryopoiesis, erythropoiesis, BM function, and peripheral blood profiles. These norma...
78 CitationsSource
#1Vincenzo Martino (University of Parma)H-Index: 3
#2Annalisa BiancheraH-Index: 11
Last. Roberto SalaH-Index: 24
view all 11 authors...
: The translocation t(9;11)(p22;q23) generates the MLL-AF9 oncogene and is commonly associated with monocytic acute myeloid leukemia (AML-M5; FAB-classification). For the oncogenicity of MLL-AF9, the (over)expression of several other genes, including selected HOXA cluster genes as well as MEIS1 (a HOX cofactor), is required. We previously showed that the down-regulation of MLL-AF9 expression is not obligatory for monocyte-macrophage maturation in AML-M5 cells carrying t(9;11)(p22;q23). In this s...
4 CitationsSource
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