Ikuo Tooyama
Shiga University of Medical Science
AnatomyInternal medicineEndocrinologyPathologyMolecular biologyNeuroscienceReceptorChemistryCholine acetyltransferaseImmunologyImmunohistochemistryIn vivoMicrogliaHippocampusAlzheimer's diseaseCentral nervous systemBiochemistryMedicineBiologyAmyloidCell biology
366Publications
52H-index
7,033Citations
Publications 364
Newest
#1Anarmaa Mendsaikhan (Shiga University of Medical Science)H-Index: 5
#2Ikuo Tooyama (Shiga University of Medical Science)H-Index: 52
Last. Douglas G. Walker (ASU: Arizona State University)H-Index: 73
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Alzheimer disease (AD) is a progressive neurodegenerative disease causing cognitive decline in the aging population. To develop disease-modifying treatments, understanding the mechanisms behind the pathology is important, which should include observations using human brain samples. We reported previously on the association of lysosomal proteins progranulin (PGRN) and prosaposin (PSAP) with amyloid plaques in non-demented aged control and AD brains. In this study, we investigated the possible inv...
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#1Shogo Higaki (Rits: Ritsumeikan University)H-Index: 6
#1Shogo Higaki (Rits: Ritsumeikan University)H-Index: 9
Last. Noriyoshi Sakai (National Institute of Genetics)H-Index: 22
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Fishes expressing a fluorescent protein in germ cells are useful to perform germ cell transfer experiments for conservation study. However, no such fish has been generated in endangered endemic fishes. In this study, we tried to generate a fish expressing Venus fluorescent protein in germ cells using Honmoroko (Gnathopogon caerulescens), which is one of the threatened small cyprinid endemic to ancient Lake Biwa in Japan. To achieve germ cell-specific expression of Venus, we used piwil1 (formally...
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#1Zulzikry Hafiz Abu Bakar (Shiga University of Medical Science)
#2Tomoko Kato (Shiga University of Medical Science)H-Index: 5
Last. Ikuo Tooyama (Shiga University of Medical Science)H-Index: 52
view all 6 authors...
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#1Natsumi Yamaguchi (Rits: Ritsumeikan University)H-Index: 1
#2Toshinori Sawano (Rits: Ritsumeikan University)H-Index: 6
Last. Hidekazu Tanaka (Rits: Ritsumeikan University)H-Index: 17
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Cerebral infarction causes motor, sensory, and cognitive impairments. Although rehabilitation enhances recovery of activities of daily living after cerebral infarction, its mechanism remains elusive due to the lack of reproducibility and low survival rate of brain ischemic model animals. Here, to investigate the relationship between rehabilitative intervention, motor function, and pathophysiological remodeling of the tissue in the ipsilateral hemisphere after cerebral infarction, we took advanta...
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#1Daijiro Yanagisawa (Shiga University of Medical Science)H-Index: 17
#2Hamizah Shahirah Hamezah (Shiga University of Medical Science)H-Index: 5
Last. Ikuo Tooyama (Shiga University of Medical Science)H-Index: 52
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Tau, a family of microtubule-associated proteins, forms abnormal intracellular inclusions, so-called tau pathology, in a range of neurodegenerative diseases collectively known as tauopathies. The rTg4510 mouse model is a well-characterized bitransgenic F1 hybrid mouse model of tauopathy, which was obtained by crossing a Camk2α-tTA mouse line (on a C57BL/6 J background) with a tetO-MAPT*P301L mouse line (on a FVB/NJ background). The aim of this study was to investigate the effects of the genetic ...
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#1Takao Morimune (Shiga University of Medical Science)H-Index: 3
#2Ayami Tano (Shiga University of Medical Science)H-Index: 2
Last. Masaki Mori (Shiga University of Medical Science)H-Index: 131
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It is not fully understood how enzymes are regulated in the tiny reaction field of a cell. Several enzymatic proteins form cytoophidia, a cellular macrostructure to titrate enzymatic activities. Here, we show that the epileptic encephalopathy-associated protein Tbc1d24 forms cytoophidia in neuronal cells both in vitro and in vivo. The Tbc1d24 cytoophidia are distinct from previously reported cytoophidia consisting of inosine monophosphate dehydrogenase (Impdh) or cytidine-5'-triphosphate synthas...
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#1Aslina Pahrudin Arrozi (Shiga University of Medical Science)
#2Daijiro Yanagisawa (Shiga University of Medical Science)H-Index: 17
Last. Ikuo Tooyama (Shiga University of Medical Science)H-Index: 52
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#2Hitomi HamaokaH-Index: 3
Last. Yoichi Kondo (Shiga University of Medical Science)
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Lactoferrin (Lf) is an iron-binding glycoprotein mainly found in exocrine secretions and the secondary granules of neutrophils. In the central nervous system (CNS), expression of the Lf protein has been reported in the lesions of some neurodegenerative disorders such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis, as well as in the aged brain. Lf is primarily considered an iron chelator, protecting cells from potentially toxic iron or iron-requiring microorganisms...
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#1Daijiro YanagisawaH-Index: 17
Last. Ikuo TooyamaH-Index: 52
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Recent evidence suggests that the formation of soluble amyloid β (Aβ) aggregates with high toxicity, such as oligomers and protofibrils, is a key event that causes Alzheimer’s disease (AD). However, understanding the pathophysiological role of such soluble Aβ aggregates in the brain in vivo could be difficult due to the lack of a clinically available method to detect, visualize, and quantify soluble Aβ aggregates in the brain. We had synthesized a novel fluorinated curcumin derivative with a fix...
1 CitationsSource
#1Daijiro Yanagisawa (Shiga University of Medical Science)H-Index: 17
#2Tomoko Kato (Shiga University of Medical Science)H-Index: 5
Last. Ikuo Tooyama (Shiga University of Medical Science)H-Index: 52
view all 11 authors...
Abstract The accumulation of β-amyloid (Aβ) aggregates in the brain occurs early in the progression of Alzheimer’s disease (AD), and non-fibrillar soluble Aβ oligomers are particularly neurotoxic. During binding to Aβ fibrils, curcumin, which can exist in an equilibrium state between its keto and enol tautomers, exists predominantly in the enol form, and binding activity of the keto form to Aβ fibrils is much weaker. Here we described the strong binding activity the keto form of curcumin derivat...
1 CitationsSource