Emanuele Giordano
University of Bologna
Ornithine decarboxylaseEndocrinologyBiomedical engineeringMolecular biologyChemistryApoptosisMaterials scienceMesenchymal stem cellMyocyteNitric oxide synthaseStem cellPopulationTissue engineeringNitric oxideIschemiaBiochemistryMedicineCell cultureBiologyCell biology
111Publications
22H-index
1,537Citations
Publications 130
Newest
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#2Joseph LovecchioH-Index: 5
Last. Giovanna Della PortaH-Index: 23
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Tissue engineering strategies can be relevant for cartilage repair and regeneration. A collagen matrix was functionalized with the addition of poly-lactic-co-glycolic acid microcarriers (PLGA-MCs) carrying a human Transforming Growth Factor β1 (hTFG-β1) payload, to provide a 3D biomimetic environment with the capacity to direct stem cell commitment towards a chondrogenic phenotype. PLGA-MCs (mean size 3 ± 0.9 μm) were prepared via supercritical emulsion extraction technology and tailored to sust...
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#1Alice Pasini (UNIBO: University of Bologna)H-Index: 9
#2Joseph Lovecchio (UNIBO: University of Bologna)H-Index: 5
Last. Emanuele Giordano (UNIBO: University of Bologna)H-Index: 22
view all 8 authors...
Conventional 2D cell culture, a traditional tool in pre-clinical studies, can hardly be regarded as a representation of a natural cell microenvironment. In this respect, it might result in altered cellular behaviors. To overcome such a limitation, different approaches have been tested to conduct more representative in vitro studies. In particular, the use of 3D cell culture introduces variables, such as cell-cell and cell-extracellular matrix interactions; cell features such as survival, prolife...
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#1Marco GovoniH-Index: 15
#2Claudio Muscari (UNIBO: University of Bologna)H-Index: 27
Last. Emanuele Giordano (UNIBO: University of Bologna)H-Index: 22
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Abstract Purpose The aim of this study was to evaluate the effects exerted over chondrogenic commitment of human adipose-derived mesenchymal stem cells (ADSCs) by a very low oxygen tension ( Materials/methods Cell morphology, mRNA levels of chondrocyte-specific marker genes and the involvement of p38 MAPK signalling were monitored in human ADSCs under a very low oxygen tension. Results Cell morphology was significantly changed after two days of hypoxic preconditioning when they featured as elong...
2 CitationsSource
#1Marilisa Cortesi (UNIBO: University of Bologna)H-Index: 5
#2Chiara LiveraniH-Index: 12
Last. Emanuele Giordano (UNIBO: University of Bologna)H-Index: 22
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Abstract Computational models constitute a fundamental asset for cancer research and drug R & D , as they provide controlled environments for testing of hypotheses and are characterized by the total knowledge of the system. These features are particularly useful for 3D cell culture models where a complex interaction among cells and their environments ensues. In this work, we present a programmable simulator capable of reproducing the behavior of cells cultured in 3D scaffolds and their response ...
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#1Marilisa Cortesi (UNIBO: University of Bologna)H-Index: 5
#2Chiara LiveraniH-Index: 12
Last. Emanuele Giordano (UNIBO: University of Bologna)H-Index: 22
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3D cell cultures are in-vitro models representing a significant improvement with respect to traditional monolayers. Their diffusion and applicability, however, are hampered by the complexity of 3D systems, that add new physical variables for experimental analyses. In order to account for these additional features and improve the study of 3D cultures, we here present SALSA (ScAffoLd SimulAtor), a general purpose computational tool that can simulate the behavior of a population of cells cultured i...
4 CitationsSource
#1Maria Camilla Ciardulli (UNISA: University of Salerno)H-Index: 5
#2Luigi Marino (UNISA: University of Salerno)H-Index: 5
Last. Giovanna Della Porta (UNISA: University of Salerno)H-Index: 23
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We developed a (three-dimensional) 3D scaffold, we named HY-FIB, incorporating a force-transmission band of braided hyaluronate embedded in a cell localizing fibrin hydrogel and poly-lactic-co-glycolic acid (PLGA) nanocarriers as transient components for growth factor controlled delivery. The tenogenic supporting capacity of HY-FIB on human-Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) was explored under static conditions and under bioreactor-induced cyclic strain conditions. HY-FIB elasticity e...
7 CitationsSource
#1Giovanna Picone (UNIBO: University of Bologna)H-Index: 2
#2Concettina Cappadone (UNIBO: University of Bologna)H-Index: 14
Last. Stefano Iotti (UNIBO: University of Bologna)H-Index: 30
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In this study, we explore the behaviour of intracellular magnesium during bone phenotype modulation in a 3D cell model built to mimic osteogenesis. In addition, we measured the amount of magnesium in the mineral depositions generated during osteogenic induction. A two-fold increase of intracellular magnesium content was found, both at three and seven days from the induction of differentiation. By X-ray microscopy, we characterized the morphology and chemical composition of the mineral deposition...
7 CitationsSource
#1Alice ZamagniH-Index: 5
#1Alice ZamagniH-Index: 8
Last. Chiara MolinariH-Index: 10
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Cisplatinpemetrexed is a frequently adopted firstline treatment for patients with advanced nonsmall cell lung cancer (NSCLC) ineligible for biological therapy, notwithstanding its limited efficacy. In the present study, the RAL cell line, an epidermal growth factor receptor (EGFR)wildtype, p53 and KRASmutated model of NSCLC, was used to investigate novel biomarkers of resistance to this treatment. Cells were analyzed 96 h (96 hpost wo) and 21 days (21 dpost wo) after the combined treatment washo...
2 CitationsSource
#1Marilisa Cortesi (UNIBO: University of Bologna)H-Index: 5
#2Chiara LiveraniH-Index: 12
Last. Emanuele Giordano (UNIBO: University of Bologna)H-Index: 22
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Epithelial to mesenchymal transition (EMT) is a complex biological process that plays a key role in cancer progression and metastasis formation. Its activation results in epithelial cells losing adhesion and polarity and becoming capable of migrating from their site of origin. At this step the disease is generally considered incurable. As EMT execution involves several individual molecular components, connected by nontrivial relations, in vitro techniques are often inadequate to capture its comp...
5 CitationsSource