Andrew J. Spracklen
Roy J. and Lucille A. Carver College of Medicine
EnhancerActinAdapter molecule crkCell migrationCytoskeletonActin remodeling of neuronsOogenesisCell fate determinationDrosophila embryogenesisArp2/3 complexCytoplasmGenetic screenDrosophila melanogasterProstaglandinActin cytoskeletonFilamentous actinActin remodelingMDia1MyosinBiologyCell biology
13Publications
7H-index
175Citations
Publications 11
Newest
#1Andrew J. Spracklen (Roy J. and Lucille A. Carver College of Medicine)H-Index: 7
#2Maureen C Lamb (Roy J. and Lucille A. Carver College of Medicine)H-Index: 4
Last. Tina L. Tootle (Roy J. and Lucille A. Carver College of Medicine)H-Index: 20
view all 4 authors...
Prostaglandins (PGs) are lipid signaling molecules with numerous physiologic functions, including pain/inflammation, fertility, and cancer. PGs are produced downstream of cyclooxygenase (COX) enzymes, the targets of non-steroidal anti-inflammatory drugs (NSAIDs). In numerous systems, PGs regulate actin cytoskeletal remodeling, however, their mechanisms of action remain largely unknown. To address this deficiency, we undertook a pharmaco-genetic interaction screen during late-stage Drosophila oog...
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#1Andrew J. Spracklen (UNC: University of North Carolina at Chapel Hill)H-Index: 7
#2Emma M. Thornton-Kolbe (UNC: University of North Carolina at Chapel Hill)H-Index: 1
Last. Mark Peifer (UNC: University of North Carolina at Chapel Hill)H-Index: 81
view all 7 authors...
: Small Src homology domain 2 (SH2) and 3 (SH3) adapter proteins regulate cell fate and behavior by mediating interactions between cell surface receptors and downstream signaling effectors in many signal transduction pathways. The CT10 regulator of kinase (Crk) family has tissue-specific roles in phagocytosis, cell migration, and neuronal development and mediates oncogenic signaling in pathways like that of Abelson kinase. However, redundancy among the two mammalian family members and the positi...
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#1Tina L. TootleH-Index: 20
#2Darren S. Hoffmann (UI: University of Iowa)H-Index: 7
Last. Daniel Kelpsch (UI: University of Iowa)H-Index: 1
view all 6 authors...
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#1Edward M. Rogers (UNC: University of North Carolina at Chapel Hill)H-Index: 12
#2Andrew J. Spracklen (UNC: University of North Carolina at Chapel Hill)H-Index: 7
Last. Mark Peifer (UNC: University of North Carolina at Chapel Hill)H-Index: 81
view all 9 authors...
Abelson family kinases (Abls) are key regulators of cell behavior and the cytoskeleton during development and in leukemia. Abl’s SH3, SH2, and tyrosine kinase domains are joined via a linker to an F-actin–binding domain (FABD). Research on Abl’s roles in cell culture led to several hypotheses for its mechanism of action: 1) Abl phosphorylates other proteins, modulating their activity, 2) Abl directly regulates the cytoskeleton via its cytoskeletal interaction domains, and/or 3) Abl is a scaffold...
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#1Andrew J. Spracklen (UNC: University of North Carolina at Chapel Hill)H-Index: 7
#2Mark Peifer (UNC: University of North Carolina at Chapel Hill)H-Index: 81
Linkage of the actomyosin cytoskeleton to cell-cell junctions drives cell shape change in development and homeostasis. In this issue of Developmental Cell, Jodoin et al. (2015) provide new insights into the underlying mechanisms, revealing that factors driving actin filament disassembly and thus dynamics also play key roles in apical constriction.
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#1Andrew J. Spracklen (Roy J. and Lucille A. Carver College of Medicine)H-Index: 7
#2Tina L. Tootle (Roy J. and Lucille A. Carver College of Medicine)H-Index: 20
Prostaglandin (PG) synthesis and signaling are conserved in Drosophila melanogaster. PGs are produced downstream of cyclooxygenase or COX enzymes, the targets of nonsteroidal anti-inflammatory drugs. Almost 20 years ago, biochemical studies suggested that Drosophila possess COX activity. Recent efforts utilizing a combination of pharmacological and genetic approaches revealed that PGs have critical functions in Drosophila oogenesis or follicle development. Pxt was identified as the COX-like enzy...
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#1Andrew J. Spracklen (Roy J. and Lucille A. Carver College of Medicine)H-Index: 7
#2Tiffany N. Fagan (Roy J. and Lucille A. Carver College of Medicine)H-Index: 3
Last. Tina L. Tootle (Roy J. and Lucille A. Carver College of Medicine)H-Index: 20
view all 4 authors...
Dynamic remodeling of the actin cytoskeleton is required for both development and tissue homeostasis. While fixed image analysis has provided significant insight into such events, a complete understanding of cytoskeletal dynamics requires live imaging. Numerous tools for the live imaging of actin have been generated by fusing the actin-binding domain from an actin-interacting protein to a fluorescent protein. Here we comparatively assess the utility of three such tools – Utrophin, Lifeact, and F...
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#1Andrew J. Spracklen (Roy J. and Lucille A. Carver College of Medicine)H-Index: 7
#2Daniel J. Kelpsch (Roy J. and Lucille A. Carver College of Medicine)H-Index: 5
Last. Tina L. Tootle (Roy J. and Lucille A. Carver College of Medicine)H-Index: 20
view all 5 authors...
Prostaglandins (PGs)—lipid signals produced downstream of cyclooxygenase (COX) enzymes—regulate actin dynamics in cell culture and platelets, but their roles during development are largely unknown. Here we define a new role for Pxt, the Drosophila COX-like enzyme, in regulating the actin cytoskeleton—temporal restriction of actin remodeling during oogenesis. PGs are required for actin filament bundle formation during stage 10B (S10B). In addition, loss of Pxt results in extensive early actin rem...
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#1Andrew J. Spracklen (Roy J. and Lucille A. Carver College of Medicine)H-Index: 7
#2Tina L. Tootle (Roy J. and Lucille A. Carver College of Medicine)H-Index: 20
Drosophila oogenesis or follicle development has been widely used to advance the understanding of complex developmental and cell biologic processes. This methods paper describes how to isolate mid-to-late stage follicles (Stage 10B-14) and utilize them to provide new insights into the molecular and morphologic events occurring during tight windows of developmental time. Isolated follicles can be used for a variety of experimental techniques, including in vitro development assays, live imaging, m...
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