Karen M. Ricketts
United States Army Medical Research Institute of Chemical Defense
Internal medicineEndocrinologyDorsumSurgeryPathologyMolecular biologyProinflammatory cytokineTumor necrosis factor alphaProteasesChemistryIn vivoSystemic administrationBiopsyCombination drugNeurogenic inflammationSubstance PChemotherapyPrednisoloneCorticosteroidCorneaInflammationEnzyme inhibitorCysteine proteaseSalineAcetylcysteineRatónAntioxidantCefazolinCytokineTriamcinolone acetonideHairlessIndometacinAntidoteCapsaicinSulfur mustardEdemaElastaseTryptaseCathepsin BSkin InjuryMucolytic AgentToxicityAnesthesiaNecrosisCathepsin HGlutathioneHistopathologyBiochemistryCalpainMedicineBiomarker (medicine)Pharmacology
8Publications
6H-index
255Citations
Publications 8
Newest
#1Michael C. Babin (United States Army Medical Research Institute of Chemical Defense)H-Index: 12
#2Karen M. Ricketts (United States Army Medical Research Institute of Chemical Defense)H-Index: 6
Last. John J. BrozettiH-Index: 1
view all 6 authors...
The eye is considered to be one of the most sensitive organs to sulfur mustard [bis(2‐chloroethyl) sulfide (SM)], with injuries ranging from mild conjunctivitis to advanced corneal disease. Even mild ocular involvement from sulfur mustard exposure can result in both physical and psychological incapacitation. In this study we explored the use of Food and Drug Administration (FDA) approved medications (prednisolone acetate ophthalmic suspension, triamcinolone, and cefazolin) as ocular treatments f...
8 CitationsSource
#1Michael C. Babin (Battelle Memorial Institute)H-Index: 12
#2Karen M. RickettsH-Index: 6
Last. Robert P. Casillas (Battelle Memorial Institute)H-Index: 20
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The mouse ear vesicant model (MEVM) is a screening tool used to identify protective compounds against acute sulfur mustard (SM)‐induced skin injury. It provides endpoints of edema and histopathology 24 h following a topical SM exposure to assess protection against inflammation and tissue damage. To further evaluate successful compounds, the MEVM was modified for use as a 7‐day model. Dose response studies were conducted with SM to select an optimal challenge dose for the new model. Due to severi...
5 CitationsSource
#1Adina Amir (Israel Institute for Biological Research)H-Index: 16
#2Tamar Kadar (Israel Institute for Biological Research)H-Index: 26
Last. M. RossH-Index: 1
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Sulfur mustard (2,2‐dichlorodiethyl sulfide; HD), is a potent alkylating agent which in liquid or vapor form is capable of causing severe injuries to skin and respiratory tract, and was shown to cause short‐ and long‐term ocular injuries. N‐Acetylcysteine (NAC) may act as a mucolytic agent, changing the “wetting” and scavenging properties of the cornea and thus the adhesion of HD. Moreover, NAC is a scavenger of HD, an antioxidant and a glutathione precursor, which was shown to reduce HD toxicit...
4 CitationsSource
#1Thomas P. Logan (United States Army Medical Research Institute of Chemical Defense)H-Index: 6
#1Thomas P. Logan (United States Army Medical Research Institute of Chemical Defense)H-Index: 2
Last. Robert P. Casillas (United States Army Medical Research Institute of Chemical Defense)H-Index: 20
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The CD1-haired mouse and the SKH-hairless mouse are two animal models that have been used to evaluate sulfur mustard (HD) exposure and protection in our laboratory. In a recent study we observed that a substance P inhibitor protected the haired mouse ear against an HD solution, but the same drug was not successful in protecting the hairless mouse against HD vapor. This experiment prompted us to compare HD exposures between these models. We determined the 14C content in the skin after exposures t...
2 CitationsSource
#1Michael C. Babin (United States Army Medical Research Institute of Chemical Defense)H-Index: 12
#2Karen M. Ricketts (United States Army Medical Research Institute of Chemical Defense)H-Index: 6
Last. Robert P. Casillas (United States Army Medical Research Institute of Chemical Defense)H-Index: 20
view all 6 authors...
The mouse ear vesicant model (MEVM) provides a quantitative edema response as well as histopathological and biochemical endpoints as measurements of inflammation and tissue damage following exposure to the chemical warfare agent sulfur mustard (HD). In the MEVM, several topically applied anti-inflammatory agents provided a significant degree of protection against HD-induced edema and dermal-epidermal separation. This study evaluated the protective effects of three of these pharmacological compou...
75 CitationsSource
#1Karen M. Ricketts (United States Army Medical Research Institute of Chemical Defense)H-Index: 6
#2C. T. Santai (United States Army Medical Research Institute of Chemical Defense)H-Index: 1
Last. Robert P. Casillas (United States Army Medical Research Institute of Chemical Defense)H-Index: 20
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Assessment of anti-inflammatory therapies against sulfur-mustard (bis(2-chloroethyl)sulfide, HD)-induced skin injury has mainly relied on qualitative histopathological evaluation. Development of quantifiable inflammatory biomarkers using fast and reliable molecular methods is needed for screening anti-inflammatory drugs against HD injury. In this study, we used two different HD exposure models to determine the in vivo cutaneous response of the inflammatory cytokines interleukin-6 (IL-6), IL-lα, ...
94 CitationsSource
#1James C. Powers (Georgia Institute of Technology)H-Index: 63
#2Chih-Min Kam (Georgia Institute of Technology)H-Index: 25
Last. Robert P. CasillasH-Index: 20
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Tissue homogenates from mouse ear skin exposed to sulfur mustard (HD, which is a military designation and probably originated from a World War I slang term Hun Stuff') were assayed for serine and cysteine protease activities. Enzyme activity was measured using synthetic chromogenic thioester and fluorogenic 7-amino-4-methylcoumarin (AMC) substrates. The tissue samples were obtained from animals (n = 6) at 3, 6, 12 and 24 h post-exposure from the right ear (HD exposed), whereas control samples we...
28 CitationsSource
#1Robert P. Casillas (United States Army Medical Research Institute of Chemical Defense)H-Index: 20
#2Robyn C. Kiser (Battelle Memorial Institute)H-Index: 9
Last. James A. Blank (Battelle Memorial Institute)H-Index: 6
view all 10 authors...
The mouse ear edema model is recognized for its usefulness in studying skin responses and damage following exposure to chemical irritants, and for evaluating pharmacological agents against chemically induced skin injury. We recently modified the mouse ear edema model for use with sulfur mustard (HD) and used this model to study the protective effect of 33 topically applied compounds comprising five pharmaceutical strategies (anti-inflammatories, protease inhibitors, scavengers/chelators, poly(AD...
102 CitationsSource