Gokhan Yahioglu
Imperial College London
Fluorescence anisotropyAnalytical chemistryCancerBiophysicsViscosityMicroviscosityPathologyAntibodyNanotechnologyLight-emitting diodeChemistryMaterials scienceConjugateIn vitroMUC1PorphyrinDrugPhotodynamic therapyCancer researchFluorescence-lifetime imaging microscopyMedicinePhotochemistryFluorescence
Publications 62
#1Mahendra Deonarain (Imperial College London)H-Index: 19
#2Gokhan Yahioglu (Imperial College London)H-Index: 24
Introduction: Antibody-Drug Conjugates (ADCs) have undergone a recent resurgence with 5 product approvals over the last 2 years but for those close to the field, it’s been repeated cycles of setbac...
3 CitationsSource
#1Mahendra DeonarainH-Index: 19
#2Gokhan YahiogluH-Index: 24
Last. Quinn XueH-Index: 1
view all 12 authors...
Antibody Drug Conjugates (ADCs) are failing due to 3 critical limitations: Low potency, ineffective solid-tumour penetration and poor tolerability. The industry is full of approaches where full-length Immunoglobulins have been engineered to carry defined numbers of payloads with higher-loadings of less potent payloads appearing to be well-tolerated. However, antibody fragments (e.g. single-chain Fcs-scFvs), which have many advantages including rapid tumour penetration, faster clearance, inexpens...
2 CitationsSource
#1I. Emilie Steinmark ('KCL': King's College London)H-Index: 2
#2Pei-Hua Chung ('KCL': King's College London)H-Index: 11
Last. Klaus Suhling ('KCL': King's College London)H-Index: 41
view all 11 authors...
Understanding viscosity in complex environments remains a largely unanswered question despite its importance in determining reaction rates in vivo. Here, time-resolved fluorescence anisotropy imaging (TR-FAIM) is combined with fluorescent molecular rotors (FMRs) to simultaneously determine two non-equivalent viscosity-related parameters in complex heterogeneous environments. The parameters, FMR rotational correlation time and lifetime, are extracted from fluorescence anisotropy decays, which in ...
4 CitationsSource
#1I. Emilie Steinmark ('KCL': King's College London)H-Index: 2
#2Cécile A. DreissH-Index: 28
Last. Gokhan Yahioglu (Imperial College London)H-Index: 24
view all 5 authors...
Here we use the combination of fluorescent molecular rotors (FMR) and fluorescence lifetime imaging (FLIM) to image matrix viscosity in live cells. We use non-cancerous, epithelial human cornea (HCE) cells as a model system. We find that mitochondrial matrix viscosity varies between individual cells and even between individual organelles, showcasing the potential of viscosity imaging for cell biology purposes.
#1Klaus SuhlingH-Index: 41
#2Yurema Teijeiro-Gonzalez ('KCL': King's College London)H-Index: 1
Last. Carla Molteni ('KCL': King's College London)H-Index: 22
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Imaging viscosity and its spatiotemporal patterns can provide valuable insight into the underlying physical conditions of biochemical reactions and biological processes in cells and tissues. One way to measure viscosity and diffusion is the use of fluorescence recovery after photobleaching (FRAP). We combine FRAP with FLIM and time-resolved fluorescence anisotropy imaging (tr-FAIM), by acquiring time- and polarization-resolved fluorescence images in every frame of a FRAP series. This allows us t...
#1I. E. Steinmark ('KCL': King's College London)H-Index: 2
#1Ida Emilie Steinmark ('KCL': King's College London)H-Index: 1
Last. Klaus Suhling ('KCL': King's College London)H-Index: 41
view all 9 authors...
The only way to visually observe cellular viscosity, which can greatly influence biological reactions and has been linked to several human diseases, is through viscosity imaging. Imaging cellular viscosity has allowed the mapping of viscosity in cells, and the next frontier is targeted viscosity imaging of organelles and their microenvironments. Here we present a fluorescent molecular rotor/FLIM framework to image both organellar viscosity and membrane fluidity, using a combination of chemical t...
16 CitationsSource
#1Ruisi Fu (Imperial College London)H-Index: 1
#2Laurence Carroll (Imperial College London)H-Index: 19
Last. Philip W. Miller (Imperial College London)H-Index: 23
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: Antibodies have long been recognised as potent vectors for carrying diagnostic medical radionuclides, contrast agents and optical probes to diseased tissue for imaging. The area of ImmunoPET combines the use of positron emission tomography (PET) imaging with antibodies to improve the diagnosis, staging and monitoring of diseases. Recent developments in antibody engineering and PET radiochemistry have led to a new wave of experimental ImmunoPET imaging agents that are based on a range of antibo...
17 CitationsSource
#1Hayley Pye (UCL: University College London)H-Index: 9
#2Mohammed A. Butt (UCL: University College London)H-Index: 9
Last. Laurence LovatH-Index: 36
view all 21 authors...
// Hayley Pye 1, * , Mohammed Adil Butt 1, 2, * , Laura Funnell 1 , Halla W. Reinert 1 , Ignazio Puccio 1 , Saif U. Rehman Khan 1 , Savvas Saouros 3, 4 , Jared S. Marklew 3 , Ioanna Stamati 3 , Maryam Qurashi 1, 4 , Rehan Haidry 2 , Vinay Sehgal 1, 2 , Dahmane Oukrif 5 , Michael Gandy 1 , Hayley C. Whitaker 1 , Manuel Rodriguez-Justo 5 , Marco Novelli 5 , Rifat Hamoudi 1, 6 , Gokhan Yahioglu 3, 4 , Mahendra P. Deonarain 1, 3, 4 and Laurence B. Lovat 1, 2 1 Department for Tissue and Energy, Divis...
5 CitationsSource
#1M P Deonarain (The Hertz Corporation)H-Index: 3
#2Gokhan Yahioglu (The Hertz Corporation)H-Index: 24
Last. Ashleigh Stewart (The Hertz Corporation)H-Index: 2
view all 8 authors...
Antibody–Drug Conjugates (ADCs) have been through multiple cycles of technological innovation since the concept was first practically demonstrated ~40 years ago. Current technology is focusing on large, whole immunoglobulin formats (of which there are approaching 100 in clinical development), many with site-specifically conjugated payloads numbering 2 or 4. Despite the success of trastuzumab-emtansine in breast cancer, ADCs have generally failed to have an impact in solid tumours, leading many t...
22 CitationsSource
#1Mohammed A. Butt (UCL: University College London)H-Index: 9
#2Hayley Pye (UCL: University College London)H-Index: 9
Last. Laurence LovatH-Index: 36
view all 16 authors...
// Mohammed Adil Butt 1, 2, * , Hayley Pye 1, * , Rehan J. Haidry 2 , Dahmane Oukrif 3 , Saif-U-Rehman Khan 1 , Ignazio Puccio 1 , Michael Gandy 1 , Halla W. Reinert 1 , Ellie Bloom 1 , Mohammed Rashid 4 , Gokhan Yahioglu 5, 6 , Mahendra P. Deonarain 1, 5, 6 , Rifat Hamoudi 1 , Manuel Rodriguez-Justo 3 , Marco R. Novelli 3 , Laurence B. Lovat 1, 2 1 Department for Tissue & Energy, University College London, London, UK 2 Upper Gastrointestinal Service, University College London Hospitals NHS Foun...
9 CitationsSource