Daniel Krowarsch
University of Wrocław
Denaturation (biochemistry)Binding siteBiophysicsCircular dichroismMutantMolecular biologyEnzymeProteasesChemistrySerineChymotrypsinTrypsinProtein structureBiochemistryProtein secondary structureStereochemistryFibroblast growth factorCrystallographyFibroblast growth factor receptorBiologyCell biology
45Publications
20H-index
1,302Citations
Publications 45
Newest
#1Martyna SochackaH-Index: 2
#2Lukasz OpalinskiH-Index: 3
Last. Malgorzata ZakrzewskaH-Index: 17
view all 8 authors...
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#1Przemysław Duda (UWr: University of Wrocław)H-Index: 5
#2Tomasz Wójtowicz (Nencki Institute of Experimental Biology)H-Index: 13
Last. Dariusz Rakus (UWr: University of Wrocław)H-Index: 25
view all 7 authors...
Long-term potentiation (LTP) is a molecular basis of memory formation. Here, we demonstrate that LTP critically depends on fructose 1,6-bisphosphatase 2 (Fbp2)—a glyconeogenic enzyme and moonlighting protein protecting mitochondria against stress. We show that LTP induction regulates Fbp2 association with neuronal mitochondria and Camk2 and that the Fbp2–Camk2 interaction correlates with Camk2 autophosphorylation. Silencing of Fbp2 expression or simultaneous inhibition and tetramerization of the...
4 CitationsSource
#1Przemysław DudaH-Index: 5
#2Tomasz WójtowiczH-Index: 13
Last. Dariusz RakusH-Index: 25
view all 7 authors...
Source
#1Martyna Sochacka (UWr: University of Wrocław)H-Index: 2
#2Lukasz Opalinski (UWr: University of Wrocław)H-Index: 3
Last. Malgorzata Zakrzewska (UWr: University of Wrocław)H-Index: 17
view all 8 authors...
Fibroblast growth factors (FGFs) via their receptors (FGFRs) transduce signals from the extracellular space to the cell interior, modulating pivotal cellular processes such as cell proliferation, motility, metabolism and death. FGF superfamily includes a group of fibroblast growth factor homologous factors (FHFs), proteins whose function is still largely unknown. Since FHFs lack the signal sequence for secretion and are unable to induce FGFR-dependent cell proliferation, these proteins were cons...
6 CitationsSource
#1Anna Szlachcic (UWr: University of Wrocław)H-Index: 10
#2Martyna Sochacka (UWr: University of Wrocław)H-Index: 2
Last. Malgorzata Zakrzewska (UWr: University of Wrocław)H-Index: 17
view all 7 authors...
Fibroblast growth factor 1 (FGF1) has been shown to interact with integrin αvβ3 through a specific binding site, involving Arg35 residue. The FGF1 mutant (R35E) with impaired integrin binding was found to be defective in its proliferative response, although it was still able to interact with FGF receptors (FGFR) and heparin and induce the activation of downstream signaling pathways. Here, we demonstrate that the lack of mitogenic potential of R35E mutant is directly caused by its decreased therm...
4 CitationsSource
It has been brought to our attention that the affiliation of Dr. Jerzy Pieczykolan at the time when he was responsible for the work described in the paper [...]
Source
#1Michal Kostas (Oslo University Hospital)H-Index: 1
#2Agata Lampart (UWr: University of Wrocław)H-Index: 2
Last. Malgorzata Zakrzewska (UWr: University of Wrocław)H-Index: 17
view all 8 authors...
Abstract FGF1 and FGF2 bind to specific cell-surface tyrosine kinase receptors (FGFRs) and activate intracellular signaling that leads to proliferation, migration or differentiation of many cell types. Besides this classical mode of action, under stress conditions, FGF1 and FGF2 are translocated in a receptor-dependent manner via the endosomal membrane into the cytosol and nucleus of the cell. However, despite many years of research, the role of translocated FGF1 and FGF2 inside the cell remains...
15 CitationsSource
#1Łukasz Opaliński (UWr: University of Wrocław)H-Index: 7
#2Jakub Szymczyk (UWr: University of Wrocław)H-Index: 2
Last. Jacek Otlewski (UWr: University of Wrocław)H-Index: 44
view all 7 authors...
Fibroblast growth factor receptor 1 (FGFR1) is a plasma membrane protein that transmits signals from the extracellular environment, regulating cell homeostasis and function. Dysregulation of FGFR1 leads to the development of human cancers and noncancerous diseases. Numerous tumors overproduce FGFR1, making this receptor a perspective target for cancer therapies. Antibody-drug conjugates (ADCs) are highly potent and selective anticancer agents. ADCs are composed of antibodies (targeting factors) ...
10 CitationsSource
#1Mateusz Adam Krzyscik (UWr: University of Wrocław)H-Index: 4
#2Malgorzata Zakrzewska (UWr: University of Wrocław)H-Index: 17
Last. Jacek Otlewski (UWr: University of Wrocław)H-Index: 44
view all 9 authors...
Antibody–drug conjugates (ADCs) are a new class of anticancer therapeutics that combine the selectivity of targeted treatment, ensured by monoclonal antibodies, with the potency of the cytotoxic agent. Here, we applied an analogous approach, but instead of an antibody, we used fibroblast growth factor 2 (FGF2). FGF2 is a natural ligand of fibroblast growth factor receptor 1 (FGFR1), a cell-surface receptor reported to be overexpressed in several types of tumors. We developed and characterized FG...
10 CitationsSource
#1Anna M. Serwotka-Suszczak (UWr: University of Wrocław)H-Index: 2
#2Alicja M. Sochaj-Gregorczyk (UWr: University of Wrocław)H-Index: 3
Last. Jacek Otlewski (UWr: University of Wrocław)H-Index: 44
view all 6 authors...
Antibody-drug conjugates (ADCs) have recently emerged as efficient and selective cancer treatment therapeutics. Currently, alternative forms of drug carriers that can replace monoclonal antibodies are under intensive investigation. Here, a cytotoxic conjugate of an anti-HER2 (Human Epidermal Growth Factor Receptor 2) diaffibody with monomethyl-auristatin E (MMAE) is proposed as a potential anticancer therapeutic. The anti-HER2 diaffibody was based on the ZHER2:4 affibody amino acid sequence. The...
11 CitationsSource