Oldřich Lapčík
Institute of Chemical Technology in Prague
AnalyteDetection limitEndocrinologySerum albuminRadioimmunoassayHigh-performance liquid chromatographyChemistryConjugateFormononetinGenisteinIsoflavonoidBiochanin AImmunoassaySteroidDaidzeinHaptenChromatographyBovine serum albuminAntiserumPolyclonal antibodies
49Publications
14H-index
674Citations
Publications 44
Newest
#1Lukas Huml (Institute of Chemical Technology in Prague)H-Index: 3
#2Dominika Havlová (Institute of Chemical Technology in Prague)H-Index: 1
Last. Michal Jurášek (Institute of Chemical Technology in Prague)H-Index: 7
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Abstract Two valuable forensic tools based on enzyme-linked immunoassays (ELISAs) for the analysis of 17α-methylated steroids were developed using haptens of stanazolol and its conjugates with biotin. Haptens containing terminal carboxylic group were conjugated to bovine serum albumin (BSA), rabbit serum albumin (RSA) or ovalbumin (OVA). Eight batches of antisera (RAbs) obtained by immunization of rabbits were tested in an indirect competitive ELISA system using immobilization of RSA conjugate (...
2 CitationsSource
#1Vladimíra Pavlíčková (Institute of Chemical Technology in Prague)H-Index: 3
#2Michal Jurášek (Institute of Chemical Technology in Prague)H-Index: 7
Last. Tomáš Ruml (Institute of Chemical Technology in Prague)H-Index: 33
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Photodynamic therapy has become a feasible direction for the treatment of both malignant and non-malignant diseases. It has been in the spotlight since FDA regulatory approval was granted to several photosensitizers worldwide. Nevertheless, there are still strong limitations in the targeting specificity that is vital to prevent systemic toxicity. Here, we report the synthesis and biological evaluation of a novel bimodal oxime conjugate composed of a photosensitizing drug, red-emitting pheophorbi...
6 CitationsSource
#1Barbora Holubová (Institute of Chemical Technology in Prague)H-Index: 4
#2Petra Mikšátková (Institute of Chemical Technology in Prague)H-Index: 7
Last. Ladislav Fukal (Institute of Chemical Technology in Prague)H-Index: 9
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Anabolic androgenic steroid contaminations in food supplements are common, and their consumption may lead to positive doping results. One of such anabolic steroid is mesterolone selected as an analyte for the present study. Some contaminated supplements cannot be detected by traditional methods due to the matrix effect. Two variants of indirect competitive immunochemical method—an enzyme-linked immunosorbent assay and an immunochromatographic method were developed using rabbit polyclonal antibod...
1 CitationsSource
#1Anna Šuláková (Institute of Chemical Technology in Prague)H-Index: 2
#2Lucie Fojtíková (Institute of Chemical Technology in Prague)H-Index: 3
Last. Martin Kuchař (Institute of Chemical Technology in Prague)H-Index: 10
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Abstract Introduction The use of new psychoactive substances as drugs of abuse has dramatically increased over the last years. Hallucinogenic phenethylamines gained particular popularity as they have both stimulating and psychedelic effects. Although generally perceived as safe, these illicit drugs pose a serious health risk; they have been linked to cases of severe poisoning or even deaths. Therefore, simple, cost-effective and reliable methods are needed for rapid determination of abused hallu...
6 CitationsSource
#1Sandra Sýkorová (Institute of Chemical Technology in Prague)H-Index: 1
#2Lucie Fojtíková (Institute of Chemical Technology in Prague)H-Index: 3
Last. Barbora Holubová (Institute of Chemical Technology in Prague)H-Index: 4
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ABSTRACTMethandienone is a synthetic exogenous steroid which, like other anabolic steroids, is strictly regulated in many countries. In recent years, increasing numbers have been detected of illegal additions into dietary supplements of methandienone and other anabolic androgenic steroids (AAS). In this work, a competitive indirect enzyme-linked immunosorbent assay (ELISA) has been constructed for the detection of methandienone using an antiserum against methandienone. Under optimal experimental...
2 CitationsSource
#1Michal Maryška (Institute of Chemical Technology in Prague)H-Index: 3
#2Lucie Fojtíková (Institute of Chemical Technology in Prague)H-Index: 3
Last. Martin Kuchař (Institute of Chemical Technology in Prague)H-Index: 10
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Tryptamines are a group of hallucinogenic drugs whose detection in body fluids could be simplified by immunochemical assay kits. Antibodies for these assays are obtained by the immunization of laboratory animals with conjugates of a hapten similar to the target analyte and a suitable protein. Therefore we synthesized novel haptens derived from tryptamine-based drugs, with N,N-dimethyltryptamine (DMT), 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) and N,N-diisopropyltryptamine (DiPT) selected as t...
1 CitationsSource
The development of polyclonal antibody-based enzyme-linked immunosorbent assays (ELISA) for the determination of individual isoflavones, i.e. daidzein, genistein and biochanin and their homologues, is presented in this work. Isoflavone conjugates with bovine serum albumin were used as immunogens, coupled at the position C 7 and C 4' via a carboxy methyl spacer. The developed ELISAs are highly specific, I 5 0 values of the standard curves range between 0.3-1.2 ng/ml. The cross reactivities to oth...
2 CitationsSource
#1Barbora HolubováH-Index: 4
#1B. HolubováH-Index: 1
Last. Ladislav FukalH-Index: 9
view all 7 authors...
Holubova B., Goselova S., Sevcikova L., Vlach M., Blažkova M., Lapcik O., Fukal L. (2013): Rapid immunoassays for detection of anabolic nortestosterone in dietary supplements. Czech J. Food Sci., 31: 514–519. An enzyme immunoassay (ELISA) and an immunochromatographic strip were designed for a rapid detection of nortestosterone in dietary supplements. Two polyclonal antibodies and two types of nortestosterone-protein coating conjugates were tested to develop the most appropriate method. Under opt...
2 CitationsSource
#1L. SosvorováH-Index: 1
#2Petra LankováH-Index: 2
Last. O. LapčíkH-Index: 1
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Sosvorova L., Lankova P., Bicikova M., Prokudina E.A., Al Malarik N., Lapcik O. (2011): ELISA for free S-equol in human urine. Czech J. Food Sci., 29: 57–64. The popularity of phytoestrogen food supplements of plant origin steadily increases. They are used to solve the problems related to the climacterium. Their efficiency depends on woman‘s capability to metabolise the active components – isoflavonoids. Women able to convert daidzein to S-equol are supposed to get a greater benefit from these s...
2 CitationsSource
#1Lucie Fojtíková (Institute of Chemical Technology in Prague)H-Index: 3
#2Anna Šuláková (Institute of Chemical Technology in Prague)H-Index: 2
Last. Ladislav Fukal (Institute of Chemical Technology in Prague)H-Index: 9
view all 8 authors...
Abstract In recent years, the use of synthetic cannabinoids (SCs) as drugs of abuse has greatly increased. SCs are associated with a risk of severe poisoning or even death. Therefore, more rapid, cost effective and reliable methods are needed, especially for the screening of drivers after traffic accidents and for detailed toxicological analysis in forensic laboratories. In this study, we developed a lateral flow immunoassay (LFIA) and an enzyme linked immunosorbent assay (ELISA) for the detecti...
9 CitationsSource
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