Luca Pierelli
Sapienza University of Rome
CD34HaematopoiesisEndocrinologyPeripheral blood mononuclear cellSurgeryOncologyMolecular biologyProgenitor cellImmunologyChemotherapyHematopoietic stem cell transplantationGranulocyte colony-stimulating factorBlood cellBone marrowStem cellTransplantationMedicineCell cultureBiologyCell biology
Publications 185
Given the availability and efficacy of the mobilizing agent plerixafor in augmenting hematopoietic progenitor cell mobilization with granulocyte colony-stimulating factor (G-CSF), there is a strong case for comparing the cost-effectiveness of mobilization with G-CSF + cyclophosphamide versus G-CSF alone. This study investigated the cost and effectiveness (i.e., successful 4 million-CD34+ collection) of G-CSF alone versus high-dose cyclophosphamide (4 g/m2) + G-CSF mobilization (± on-demand pleri...
#1Gwen Clarke (Canadian Blood Services)H-Index: 11
#2Melanie Bodnar (Alexandra Hospital)
Last. Lani Lieberman (UHN: University Health Network)H-Index: 14
view all 29 authors...
#1Luca Pierelli (Sapienza University of Rome)H-Index: 34
Last. Michele VaccaH-Index: 3
view all 3 authors...
: Therapeutic apheresis (TA) includes a wide range of therapeutic procedures based on the separation of blood components and the collection of cells with therapeutic activity or the removal of unwanted plasma or cellular components involved in the etiology of various hematologic, renal, neurological, and medical diseases. The complexity of these interventions requires an organizing model to assure a proper clinical environment, technology, quality requirements, and personnel as well as an active...
#1Maria Michela D’Aloia (Sapienza University of Rome)H-Index: 3
#2Ilaria Grazia Zizzari (Sapienza University of Rome)H-Index: 13
Last. Maurizio Alimandi (Sapienza University of Rome)H-Index: 24
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Adoptive cell therapy of solid tumors with reprogrammed T cells can be considered the “next generation” of cancer hallmarks. CAR-T cells fail to be as effective as in liquid tumors for the inability to reach and survive in the microenvironment surrounding the neoplastic foci. The intricate net of cross-interactions occurring between tumor components, stromal and immune cells leads to an ineffective anergic status favoring the evasion from the host’s defenses. Our goal is hereby to trace the road...
159 CitationsSource
#1Federico Biscetti (CUA: The Catholic University of America)H-Index: 16
#2Stefano Gentileschi (CUA: The Catholic University of America)H-Index: 16
Last. Andrea Flex (CUA: The Catholic University of America)H-Index: 19
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Abstract Peripheral arterial disease (PAD), is a major health problem. Many studies have been focused on the possibilities of treatment offered by vascular regeneration. Human adipose-derived stem cells (HASCs), multipotent CD34+ stem cells found in the stromal-vascular fraction of adipose tissues, which are capable to differentiate into multiple mesenchymal cell types. The High mobility group box 1 protein (HMGB1) is a nuclear protein involved in angiogenesis. The aim of the study was to define...
4 CitationsSource
#1S Morton (University Hospitals Birmingham NHS Foundation Trust)H-Index: 1
#2Simon J. Stanworth (NHSBT: NHS Blood and Transplant)H-Index: 51
Last. Darrell J. Triulzi (University of Pittsburgh)H-Index: 37
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1 CitationsSource
#1Paola IudiconeH-Index: 10
#2Daniela FioravantiH-Index: 7
Last. Luca Pierelli (Sapienza University of Rome)H-Index: 34
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Abstract CIK cells are a subset of effector lymphocytes endowed with a non-MHC restricted anti-tumor activity making them an appealing and promising cell population for adoptive immunotherapy. CIK are usually generated ex-vivo by initial priming with Interferon-γ (IFN-γ) and monoclonal antibody against CD3 (anti-CD3), followed by culture in medium containing Interleukin-2 (IL-2). Interleukin-15 (IL-15) shares with IL-2 similar biological functions and recently it has been reported to induce CIK ...
5 CitationsSource
#1Raffaella FazzinaH-Index: 8
#2Paola IudiconeH-Index: 10
Last. Luca Pierelli (Sapienza University of Rome)H-Index: 34
view all 7 authors...
Background Mesenchymal stromal cells (MSCs) have been largely investigated, in the past decade, as potential therapeutic strategies for various acute and chronic pathological conditions. MSCs isolated from different sources, such as bone marrow (BM), umbilical cord tissue (UCT) and adipose tissue (AT), share many biological features, although they may show some differences on cumulative yield, proliferative ability and differentiation potential. The standardization of MSCs growth and their funct...
21 CitationsSource
#1Raffaella FazzinaH-Index: 8
#2Paola IudiconeH-Index: 10
Last. Luca PierelliH-Index: 34
view all 9 authors...
Alternatives to the use of fetal bovine serum (FBS) have been investigated to ensure xeno-free growth condition. In this study we evaluated the efficacy of human platelet lysate (PL) as a substitute of FBS for the in vitro culture of some human cell lines. PL was obtained by pools of pathogen inactivated human donor platelet (PLT) concentrates. Human leukemia cell lines (KG-1, K562, JURKAT, HL-60) and epithelial tumor cell lines (HeLa and MCF-7) were cultured with either FBS or PL. Changes in ce...
17 CitationsSource
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