Gene

Engineering

Biotechnology

Bacteria

Biology

Corynebacterium glutamicum

CRISPR

Biochemistry

Bioproduction

Computational biology

Biochemical engineering

Genetics

Corynebacterium

Enzyme

Chemistry

Metabolic engineering

Current Opinion in Biotechnology

Corynebacterium glutamicum, a natural glutamate-producing bacterium adopted for industrial production of amino acids, has been extensively explored recently for high-level biosynthesis of amino acid derivatives, bulk chemicals such as organic acids and short-chain alcohols, aromatics, and natural products, including polyphenols and terpenoids. Here, we review the recent advances with a focus on biosystem design principles, metabolic characterization and modeling, omics analysis, utilization of nonmodel feedstock, emerging CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) tools for Corynebacterium strain engineering, biosensors, and novel strains of C. glutamicum. Future research directions for developing C. glutamicum cell factories are also discussed.

Biosystem design of Corynebacterium glutamicum for bioproduction