Gene

Biology

Chalcone isomerase

Nutraceutical

Biochemistry

Computational biology

Metabolic pathway

Chalcone synthase

Biosynthesis

Antioxidant

Enzyme

Flavonoid

Flavanone

Naringenin

Chemistry

In silico

Computational and Structural Biotechnology Journal

Among the most relevant bioactive molecules family, phenolic compounds (PCs) are well known in higher plants, while their knowledge in microalgae is still scarce. Microalgae represent a novel and promising source of human health benefit compounds to be involved, for instance, in nutraceutical composition. This study aims to investigate the PCs biosynthetic pathway in the microalgal realm, exploring its potential variability over the microalgal biodiversity axis. A multistep in silico analysis was carried out using a selection of core enzymes from the pathway described in land plants. This study explores their presence in ten groups of prokaryotic and eukaryotic microalgae.. Analyses were carried out taking into account a wide selection of algal protein homologs, functional annotation of conserved domains and motifs, and maximum-likelihood tree construction. Results showed that a conserved core of the pathway for PCs biosynthesis is shared horizontally in all microalgae. Conversely, the ability to synthesize some subclasses of phenolics may be restricted to only some microalgal groups (i.e., Chlorophyta) depending on featured enzymes, such as the flavanone naringenin and other related chalcone isomerase dependent compounds.

Insights into the biosynthesis pathway of phenolic compounds in microalgae