Gene

Catalysis

Biology

Phosphine

Biochemistry

Lipid peroxidation

Antioxidant

Internal medicine

Medicine

Chemistry

TCEP

Vitellogenin

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology

In aquatic milieus, tris (2-chloroethyl) phosphate (TCEP) was detected as an emerging environmental contaminant. In this study, in vivo experiment and in-silico docking was integrated systematically to explore the toxic mechanisms of TCEP using zebrafish (Danio rerio). Fish (mean weight of 0.24 ± 0.02 g) were exposed to 100 and 1500 μg L-1 concentrations of TCEP for 28 days under the static renewal method. During chronic exposure, plasma steroid hormones such as testosterone (T) and 17β estradiol (E2), plasma vitellogenin (Vtg) and antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and lipid peroxidation (LPO) in gonads were significantly (P < 0.05) altered in TCEP exposed group (1500 μg L-1) compared to the control group. However, the alterations of these parameters were not significant on the 14th day (except Vtg and GR in testis) in 100 μg L-1 of TCEP exposed groups. There were no significant differences (p > 0.05) in the growth parameters comparing TCEP exposed groups with the control group. The gonads of fish exposed to TCEP showed significant histopathological changes when compared to the control groups. A docking study observed that TCEP possessed binding affinity with the estrogen receptor (ERβ) and androgen receptor (AR). These data indicate that TCEP at tested concentrations adversely affects the aquatic organisms.

Long term exposure to tris (2-chloroethyl) phosphate (TCEP) causes alterations in reproductive hormones, vitellogenin, antioxidant enzymes, and histology of gonads in zebrafish (Danio rerio): In vivo and computational analysis