bone formation

alp activity

Osteologie

Introduction Bone healing is reported to be disturbed by excessive oxidative stress. Nuclear factor erythroid 2-related factor 2 (Nrf2) is responsible for regulating antioxidant systems, and it also has an important role in fracture healing. Our previous study showed that Nrf2-knockout mice had reduced fracture callus compared with wild type mice, but the effect of pharmacological Nrf2 induction on bone healing is still unknown. Given that osteoblast function is generally essential for bone healing process, to investigate the effect of Nrf2-inducer on osteoblasts under oxidative stress would lead to a future therapeutic strategy for patients with refractory fracture healing. The purpose of this in vitro study is to examine the effects of Nrf2-inducer methysticin on osteoblast proliferation and mineralization under hydrogen peroxide (H2O2)-induced oxidative stress condition.

Effect of Methysticin on osteoblast function under oxidative stress through Nrf2/HO-1 signaling pathway: in vitro study