FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants

Volume: 22, Issue: 1
Published: Feb 19, 2021
Abstract
The broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we present a protoplasting-free approach, flsnRNA-seq, for large-scale full-length RNA profiling at a single-nucleus level in plants using isolated nuclei. Combined with 10x Genomics and Nanopore long-read sequencing, we validate the robustness of...
Paper Details
Title
FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants
Published Date
Feb 19, 2021
Volume
22
Issue
1
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