Gene

Somatic embryogenesis

Transformation (genetics)

Cell biology

Biology

Transgene

Agrobacterium tumefaciens

Genome editing

CRISPR

Regeneration (biology)

Transformation efficiency

Computer science

Botany

Explant culture

Computational biology

Genetically modified crops

Vigna

Genetics

Genome

Embryonic stem cell

In vitro

Organogenesis

Meristem

Shoot

Agrobacterium

Selectable marker

The Plant Journal

SUMMARY Cowpea ( Vigna unguiculata (L.) Walp.) is one of the most important legume crops planted worldwide, but despite decades of effort, cowpea transformation is still challenging due to inefficient Agrobacterium ‐mediated transfer DNA delivery, transgenic selection and in vitro shoot regeneration. Here, we report a highly efficient transformation system using embryonic axis explants isolated from imbibed mature seeds. We found that removal of the shoot apical meristem from the explants stimulated direct multiple shoot organogenesis from the cotyledonary node tissue. The application of a previously reported ternary transformation vector system provided efficient Agrobacterium ‐mediated gene delivery, while the utilization of spcN as selectable marker enabled more robust transgenic selection, plant recovery and transgenic plant generation without escapes and chimera formation. Transgenic cowpea plantlets developed exclusively from the cotyledonary nodes at frequencies of 4% to 37% across a wide range of cowpea genotypes. CRISPR/Cas‐mediated gene editing was successfully demonstrated. The transformation principles established here could also be applied to other legumes to increase transformation efficiencies.

Developing a rapid and highly efficient cowpea regeneration, transformation and genome editing system using embryonic axis explants