Pathology

Genomics

Mycobacterium tuberculosis

Tuberculosis

Biology

Gene

Gene silencing

Functional genomics

Function (biology)

Genome

Mycobacterium smegmatis

Computational biology

Genetics

Medicine

eLife

possesses a large number of genes of unknown or predicted function, undermining fundamental understanding of pathogenicity and drug susceptibility. To address this challenge, we developed a high-throughput functional genomics approach combining inducible CRISPR-interference and image-based analyses of morphological features and sub-cellular chromosomal localizations in the related non-pathogen,

Arrayed CRISPRi and quantitative imaging describe the morphotypic landscape of essential mycobacterial genes