Prevalence of oxacillin-susceptible methicillin-resistant Staphylococcus aureus nasal carriage and their clonal diversity among patients attending public health-care facilities

Published on Apr 1, 2020in Indian Journal of Medical Microbiology0.9
· DOI :10.4103/IJMM.IJMM_20_157
Aline Peçanha Muzy Dias1
Estimated H-index: 1
(Federal Fluminense University),
Lorrayne Cardoso Guimarães1
Estimated H-index: 1
+ 5 AuthorsFábio Aguiar-Alves8
Estimated H-index: 8
(Federal Fluminense University)
Sources
Abstract
Context: Nosocomial infections arise from many microorganisms, including Staphylococcus aureus. Aims: The aim of this study is to determine the molecular epidemiology of circulating methicillin-resistant S. aureus (MRSA) clones among patients attending community and health-care facilities in Nova Friburgo, RJ, Brazil. Methods: A total of 1002 nasal swab samples were collected from May 2010 to September 2015. S. aureus isolates were identified through phenotypic tests, submitted to antimicrobial susceptibility tests and genotypic analysis to detect mecA, panton-valentine leucocidin (PVL) genes, SCCmec, SPA and multilocus sequencing typing (MLST) typing. Results: We identified 294 (29.3%) isolates as S. aureus and 91 (9.1%) as MRSA. A total of 17 isolates did not present a correlation between phenotypic and genotypic resistance profiles. Among MRSA isolates, 17 (18.7%) carried PVL genes. A total of 20 different SPA types were determined, being grouped by MLST into eight different sequence types. ST5/t002 was the most prevalent genotype found among these isolates. Conclusions: There is a gradual colonisation shift happening in the infection pattern by S. aureus in Brazil. The Brazilian Epidemic Clone (ST239-SCCmec IIIa-PVL-) seems to be substituted by isolates from different clonal complexes, such as ST5, ST8 and ST30. The non-correlation between phenotypic/genotypic resistance profile observed in some isolates suggests the presence of other methicillin resistance mechanisms different from mecA presence or a difference in the nucleotide sequence, which prevents the primers to identify the specific region during polymerase chain reaction reactions. MRSA identification should be based on phenotypic and genotypic testing to ensure the various types of resistance mechanisms.
📖 Papers frequently viewed together
4 Citations
25 Citations
1 Citations
References23
Newest
#1Dina RaafatH-Index: 7
#2Daniel M. MrochenH-Index: 5
Last. Silva HoltfreterH-Index: 21
view all 20 authors...
Rats are a reservoir of human Staphylococcus aureus (MRSA). However, the composition of the natural S. aureus population in wild and laboratory rats is largely unknown. Here, 144 nasal S. aureus isolates from free-living wild rats, captive wild rats and laboratory rats were genotyped and profiled for antibiotic resistances and human-specific virulence genes. The nasal S. aureus carriage rate was higher among wild rats (23.4%) than laboratory rats (12.3%). Free-living wild rats were primarily col...
8 CitationsSource
#1Aneela KhawajaH-Index: 2
#2Faiqa ArshadH-Index: 2
Last. Idrees Khan (Panjab University, Chandigarh)H-Index: 1
view all 3 authors...
OBJECTIVE: To determine methicillin resistance in staphylococcus aureus by different phenotypic methods, and to evaluate their accuracy with mecA gene polymerase chain reaction for methicillin-resistant staphylococcus aureus detection. METHODS: The descriptive cross-sectional study was conducted from January to December 2015 at the Post- Graduate Medical Institute, Lahore, Pakistan, and comprised consecutive, non-repetitive clinical isolates of methicillin-resistant staphylococcus aureus that we...
1 CitationsSource
#1M. Angeles Argudín (ULB: Université libre de Bruxelles)H-Index: 14
#2Sandrine Roisin (ULB: Université libre de Bruxelles)H-Index: 18
Last. Olivier Denis (ULB: Université libre de Bruxelles)H-Index: 59
view all 8 authors...
: Methicillin-resistant Staphylococcus aureus isolates lacking mec genes (n = 32), collected from Belgian hospitals, were characterized for their β-lactamase production and the presence of mutations in pbp genes, the pbp4 promoter, and genes involved in penicillin-binding protein 4 overproduction (gdpP and yjbH). Twelve isolates were β-lactamase hyperproducers (BHPs), while 12 non-BHP isolates might produce an incomplete GdpP protein. Most isolates showed nucleotide missense mutations in pbp gen...
33 CitationsSource
#1Fernanda Sampaio Cavalcante (UFRJ: Federal University of Rio de Janeiro)H-Index: 10
#2Marcos Vinicius Pinheiro (UFRJ: Federal University of Rio de Janeiro)H-Index: 1
Last. Kátia Regina Netto dos Santos (UFRJ: Federal University of Rio de Janeiro)H-Index: 23
view all 7 authors...
Background Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are associated with greater mortality and morbidity; however, risk factors for community-acquired infections caused by MRSA have not been established. Therefore, community patients who are admitted to hospitals without the necessary contact precautions and are infected with community-acquired lineages eventually cause these lineages to spread to these settings. The aim of this study was to detect community-acquire...
7 CitationsSource
#1Kassandra LoewenH-Index: 2
#2Yoko Schreiber (Northern Ontario School of Medicine)H-Index: 2
Last. Len KellyH-Index: 19
view all 5 authors...
To provide information on the prevalence and treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections and the distinction between community-associated MRSA and health care-associated MRSA.The MEDLINE and EMBASE databases were searched from 2005 to 2016. Epidemiologic studies were summarized and the relevant treatment literature was based on level I evidence.The incidence of community-associated MRSA infection is rising. Certain populations, including indigenous Canadians and ho...
34 Citations
#1Maria Teresa C. Vieira (University of California, Berkeley)H-Index: 1
#2Mariel MarlowH-Index: 12
view all 10 authors...
Background:Methicillin-resistant Staphylococcus aureus (MRSA) colonization has been linked to HIV-related sexual and social behaviors. MRSA risk factors may be different for HIV-infected children, adolescents and young adults. We investigated the association of MRSA colonization, persistent coloniza
9 CitationsSource
#1Pratibha Kale (AIIMS: All India Institute of Medical Sciences)H-Index: 3
#2Benu Dhawan (AIIMS: All India Institute of Medical Sciences)H-Index: 23
Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of infection, both in hospitalised patients with significant healthcare exposure and in patients without healthcare risk factors. Community-acquired methicillin-resistant S. aureus (CA-MRSA) are known for their rapid community transmission and propensity to cause aggressive skin and soft tissue infections and community-acquired pneumonia. The distinction between the healthcare-associated (HA)-MRSA and CA-MRSA is gradually f...
21 CitationsSource
#1Mariana Andrade-Figueiredo (FIOCRUZ: Oswaldo Cruz Foundation)H-Index: 2
#2Tereza Cristina Leal-Balbino (FIOCRUZ: Oswaldo Cruz Foundation)H-Index: 11
Staphylococcus aureus is the major cause of global and nosocomial infections with a significant impact in hospitals worldwide. Our objective was to investigate clinical and molecular characteristics of S. aureus isolates causing infections in patients admitted to hospitals from Recife city, Brazil, and investigate the prevalence of oxacillin-susceptible mecA-positive S. aureus (OS-MRSA) in the region, as well as genetically characterize the isolates and compare with epidemic clones. We character...
25 CitationsSource
#1Muge Oguzkaya-Artan (Erciyes University)H-Index: 4
#2Cem ArtanH-Index: 5
Last. Zeynep Baykan (Erciyes University)H-Index: 12
view all 3 authors...
Aim: We aimed to determine the prevalence and associated risk factors for nasal methicillin‑sensitive and methicillin‑resistant Staphylococcus aureus (MSSA/MRSA) carriage among patients admitted to a chest clinic of a tertiary care hospital in this study. Materials and Methods: Nasal samples were taken from anterior nares were cultured in CHROMagar S. aureus plates, MRSA was determined by disc diffusion method (cefoxitin 30 μg) according to the Clinical and Laboratory Standards Institute guideli...
6 CitationsSource
#1Hamed Ghasemzadeh-Moghaddam (UPM: Universiti Putra Malaysia)H-Index: 8
#2Vasanthakumari Neela (UPM: Universiti Putra Malaysia)H-Index: 7
Last. A. van Belkum (BioMérieux)H-Index: 62
view all 12 authors...
Abstract We performed a prospective observational study in a clinical setting to test the hypothesis that prior colonization by a Staphylococcus aureus strain would protect, by colonization interference or other processes, against de novo colonization and, hence, possible endo-infections by newly acquired S. aureus strains. Three hundred and six patients hospitalized for >7 days were enrolled. For every patient, four nasal swabs (days 1, 3, 5, and 7) were taken, and patients were identified as c...
15 CitationsSource
Cited By1
Newest
#1Thamiris Santana Machado (Federal Fluminense University)H-Index: 2
#2Felipe Ramos Pinheiro (Federal Fluminense University)H-Index: 1
Last. Fábio Aguiar-Alves (Federal Fluminense University)H-Index: 8
view all 10 authors...
Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niteroi-Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the mec staphylococcal c...
2 CitationsSource