Metabolomics Analysis of Ammonia Secretion during the Fermentation of Klebsiella variicola GN02 with Highly Efficient Endophytic Nitrogen-Fixing Bacteria

The ammonia-secreting performance is one of the key factors for determining nitrogen-fixing capacity of the cell. Klebsiella variicola GN02 is an endophytic nitrogen-fixing bacterium isolated from the roots of mature Pennisetum giganteum z.x.lin. In order to reveal the key factor which influences the ammonia secretion in fermentation process of K. variicola, GC-TOF-MS metabolomics was used. The changes of OD600, ammonia nitrogen content and...