Molecular biology

Biology

Pulmonary artery

Endocrinology

Apoptosis

Cell growth

Biochemistry

In vitro

Myocyte

Internal medicine

Andrology

Medicine

Chemistry

Zhonghua mazuixue zazhi

Objective To evaluate the effects of high mobility group protein box 1 (HMGB1) on the proliferation,migration and apoptosis of human pulmonary artery smooth muscle cells (hPASMCs) in vitro.Methods The hPASMCs were seeded in 96-well plates in DMEM liquid culture medium (100 μl/well) with the density of 2 × 105/ml,in 6-well plate (1 ml/well) with the density of 2 × 106/ml or in modified 24-well Boyden chamber (100 μl/well) with the density of 5 × 103/ml and randomly divided into 5 groups (n =26 each):control group (group C) and 4 different concentrations of HMGB1 groups (groups H1-H4).The cells were cultured in DMEM liquid culture medium in group C.The cells were cultured in DMEM liquid culture medium containing HMGB1 1,10,100 and 1000 ng/ml in groups H1-H4,respectively.At 24 and 48 h of incubation,the proliferation,migration and apoptosis of hPASMCs were detected by MTT,modified Boyden chamber assay,and TUNEL,respectively.Results Compared with group C,the proliferation and migration of hPASMCs were significantly increased in groups H1-H4 (P ＜ 0.05).The proliferation of hPASMCs was significantly higher in groups H2-H4,and the migration of hPASMCs was higher in groups H3 and H4 than in group H1,and in groups H3 and H4 than in group H2 (P ＜ 0.05).There was no significant difference in the parameters mentioned above between groups H3 and H4 (P ＞ 0.05).The proliferation of hPASMCs was significantly higher at 48 h of incubation than at 24 h of incubation (P ＜ 0.05).There was no significant difference in the apoptotic rate between the five groups (P ＞ 0.05).Conclusion HMGB1 can induce the proliferation and migration of hPASMCs in vitro,which may be involved in pulmonary vascular remodeling during acute lung injury.Key words: High mobility group protein box 1 ;  Muscle cells, smooth muscle;  Cell proliferation; Cell movement;  Apoptosis

Effects of high mobility group protein box 1 on proliferation, migration and apoptosis of human pulmonary artery smooth muscle cells in vitro