Internal medicine

Biotechnology

Neuroscience

In vivo

Oxidative stress

Biology

Ophthalmology

Endocrinology

Apoptosis

In vitro

Immunology

Retina

Ex vivo

Inflammation

Hypothermia

Microglia

Programmed cell death

Retinal

Organ culture

Cell biology

Biochemistry

Medicine

Clinical and Experimental Ophthalmology

Background Hydrogen peroxide (H 2 O 2 ) can be used in vitro to simulate oxidative stress. In retinal organ cultures, H 2 O 2 induces strong neurodegeneration of the retina. It is known that oxidative stress plays a role in the development of several retinal diseases including glaucoma and ischemia. Thus, we investigated whether processes underlying oxidative stress can be prevented by hypothermia using an ex vivo organ culture model of porcine retinas. Methods Porcine retinal explants were cultivated for 5 and 8 days. Oxidative stress was induced via 300 μM H 2 O 2 on day 1 for 3 hours. Hypothermia treatment at 30°C was applied simultaneously with H 2 O 2 , for 3 hours. Retinal ganglion cells (RGCs), apoptosis, bipolar and cholinergic amacrine cells, microglia and macroglia were evaluated immunohistologically. Apoptosis rate was additionally analysed via western blot. Results Reduced apoptosis rates through hypothermia led to a preservation of RGCs ( P &lt; .001). Amacrine cells were rescued after hypothermia treatment ( P = .17), whereas bipolar cells were only protected partly. Additionally, at 8 days, microglial response due to oxidative stress was completely counteracted via hypothermia ( P &lt; .001). Conclusions H 2 O 2 induced strong degenerative processes in porcine retinas. The role of oxidative stress in the progression of retinal diseases makes this ex vivo organ culture model suitable to investigate new therapeutic approaches. In the present study, the damaging effect of H 2 O 2 to several retinal cell types was counteracted or strongly alleviated through hypothermia treatment. Especially RGCs, which are affected in glaucoma disease, were protected due to a reduced apoptosis rate through hypothermia.

Oxidative stress‐induced retinal damage is prevented by mild hypothermia in an ex vivo model of cultivated porcine retinas