Supercritical fluid

Physics

Internal medicine

Organic chemistry

Laser surgery

Laser

Biology

Optics

Matrix (chemical analysis)

Supercritical carbon dioxide

Biomedical engineering

Surgery

Extracellular matrix

Decellularization

Composite material

Adipose tissue

Chemistry

Materials science

Stem cell

Wound healing

Tissue engineering

Carbon dioxide laser

Cell biology

Biochemistry

Medicine

International Journal of Medical Sciences

Diabetes mellitus (DM) causes impaired wound healing by affecting one or more of the biological mechanisms of hemostasis, inflammation, proliferation, and remodeling and a large number of cell types, extracellular components, growth factors, and cytokines.Interventions targeted toward these mechanisms might accelerate the wound healing process.To evaluate the wound healing efficacy of supercritical carbon dioxide (scCO2)-decellularized porcine acellular dermal matrix (ADM) combined with autologous adipose-derived stem cells (ASCs) in streptozotocin (STZ)-induced DM rats.DM was induced by injecting rats with STZ; dorsal full-thickness skin (5 × 5 cm 2 ) was created and treated with and without ASCs-scCO2-treated ADM to evaluate the wound healing rate through histological examination, fluorescence microscopic observation, and immunohistochemical analysis.In the present study, complete decellularization of the porcine dermal matrix was achieved through scCO2.Isolation of ASCs was conducted and evaluated using CD29 + /CD31 -/CD45 -/CD90 + markers in flow cytometry, which indicated that more than 90% of cells were ASCs.The percentage of cells labeled with CD29 + and CD90 + was found to be 97.50% and 99.69%, respectively.The wound healing rate increased in all groups relative to the group with the DM wound without treatment.DM wound treated with ADM-ASCs showed significantly higher (p < 0.01) wound healing rate than DM wound without treatment.ADM-ASC-treated rats showed significantly increased epidermal growth factor, Ki67, and prolyl 4-hydroxylase and significantly decreased CD45 compared with the group with the DM wound without treatment.The intervention comprising ADM decellularized from porcine skin by using scCO2 and ASCs was proven to improve diabetic wound healing.ADM-ASCs had a positive effect on epidermal regeneration, anti-inflammation, collagen production and processing, and cell proliferation; thus, it accelerated wound healing.

Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing