Biotechnology

In vivo

Eggs per gram

Veterinary medicine

Biology

Microbiology

Animal science

Helminths

Immunology

Haemonchus contortus

Strongyloides

Feces

Medicine

3 Biotech

The objective of the present work was to evaluate the efficiency of Bioverm® fungal formulation (Duddingtonia flagrans—AC001) in controlling Haemonchus contortus and Strongyloides papillosus in sheep. In vitro predation tests were carried out in Petri dishes containing agar culture medium 2%. Four experimental groups were formed, with five replicates each: Group 1: 1 g of Bioverm® and 1000 third-stage larvae (L3) of H. contortus; Group 2: 1000 L3 of H. contortus; Group 3: 1 g of Bioverm® and 1000 L3 of S. papillosus; and Group 4: 1000 L3 of S. papillosus. In the in vivo tests, twelve 11-month-old sheep males positive for H. contortus were used. The animals were sorted in two groups (treatment and control), based on fecal egg counts (eggs per gram, EPG). Each group comprised six animals: treatment group—each animal received orally 100 g of Bioverm®; and control group—each animal received orally 100 g of rice. Subsequently, feces from these animals were collected at 12, 24, 36, 48, 60, 72, 84, and 96 h after Bioverm® administration. In vitro results demonstrate that D. flagrans kept its predatory activity with 91.5% of mean reduction percentage of L3. After the passage test, Bioverm® presented efficacy already after 12 h of its administration and kept similar results for 60 h. Bioverm® fungal formulation (D. flagrans—AC001) was efficient in reducing the population of H. contortus and S. papillosus under laboratory conditions in sheep feces. However, further studies are needed under natural conditions of ruminant grazing to prove the efficiency of this product.

Efficiency of the Bioverm® (Duddingtonia flagrans) fungal formulation to control in vivo and in vitro of Haemonchus contortus and Strongyloides papillosus in sheep