Supercritical fluid

Organic chemistry

Supercritical fluid chromatography

Complex matrix

Metabolome

Gas chromatography

Metabolomics

Chemistry

Chromatography

Bioanalysis

Metabolomics is the comprehensive study of small-molecule metabolites. Obtaining a wide coverage of the metabolome is challenging because of the broad range of physicochemical properties of the small molecules. To study the compounds of interest spectroscopic (NMR), spectrometric (MS) and separation techniques (LC, GC, supercritical fluid chromatography, CE) are used. The choice for a given technique is influenced by the sample matrix, the concentration and properties of the metabolites, and the amount of sample. This review discusses the most commonly used analytical techniques for metabolomic studies, including their advantages, drawbacks and some applications.

Analytical Techniques for Metabolomic Studies: A Review