Immunomodulatory activities of sulfated Cyclocarya paliurus polysaccharides with different degrees of substitution on mouse spleen lymphocytes

Published on Jan 1, 2020in Journal of Functional Foods3.701
· DOI :10.1016/J.JFF.2019.103706
Yue Yu6
Estimated H-index: 6
(NCU: Nanchang University),
Qianqian Song8
Estimated H-index: 8
(NCU: Nanchang University)
+ 4 AuthorsJianhua Xie35
Estimated H-index: 35
(NCU: Nanchang University)
Sources
Abstract
Abstract Two sulfated polysaccharides SCP3 and SCP5 with degree of substitution (DS) 0.45 and 0.13 were obtained by modifying the Cyclocarya paliurus polysaccharide (CP) with chlorosulfonic acid-pyridine method. Results demonstrated that the physicochemical properties, molar ratios of monosaccharide composition, molecular weights, and in vitro immunomodulatory activity were changed after being sulfated. Splenocytes proliferation assay showed that both CP and sulfated polysaccharides have immunomodulatory, while sulfated polysaccharides were stronger. CP mainly stimulate the production of the Th1-type cytokines, SCP3 and SCP5 not only significantly enhanced the secretion level of Th1-type cytokines, but also promote the Th2-type cytokines. Sulfated polysaccharides could significantly stimulate splenic lymphocyte proliferation synergistically with ConA or LPS. SCP3 significantly promoted the proliferation of T lymphocytes, while SCP5 stimulated the proliferation of B lymphocytes, which might be related to the DS. These results indicated that sulfation could improve the immune-enhancing effects of CP and different DS has different immunomodulatory activity.
📖 Papers frequently viewed together
69 Citations
128 Citations
References42
Newest
#1Liuming Xie (NCU: Nanchang University)H-Index: 2
#2Mingyue Shen (NCU: Nanchang University)H-Index: 29
Last. Jianhua Xie (NCU: Nanchang University)H-Index: 35
view all 6 authors...
Abstract With the rising trend of incidence of cancers, effective therapies are urgently needed to control human malignancies. However, the chemotherapy drugs currently on the market cause serious side effects. Polysaccharides belong to a class of biomacromolecules, which have drawn considerable research interest over the years as it possess anti-cancer activities or can increase the efficacy of conventional chemotherapy drugs with fewer side effects. The antitumor activity of many polysaccharid...
47 CitationsSource
#1Jianxi Song (Beihua University)H-Index: 3
#2Ye Wu (Beihua University)H-Index: 2
Last. Haibin Tong (Wenzhou University)H-Index: 8
view all 7 authors...
Abstract In this study, three sulfated polysaccharides (S-RSP1–2, S-RSP1–4 and S-RSP1–8) from Rhodiola sachalinensis were produced by chlorosulfonic acid-pyridine method. d -gal was used to develop an oxidative stress model in the mouse embryonic fibroblast cell line NIH 3T3. Effects of the three sulfated polysaccharides on d -gal-induced oxidative stress were investigated. The results showed that S-RSP1–4 improved the viability of the d -gal-induced oxidative stress in NIH 3T3 cells. The sulfat...
4 CitationsSource
#1Lixin Huang (NCU: Nanchang University)H-Index: 7
#2Mingyue Shen (NCU: Nanchang University)H-Index: 29
Last. Jianhua Xie (NCU: Nanchang University)H-Index: 35
view all 4 authors...
Abstract Background Polysaccharides are natural macromolecular polymers that are widely distributed in various food resources and have attracted much attention due to their significant bioactivities. Sulfated polysaccharides refer to polysaccharides containing sulfate groups on sugar units. A large number of studies have characterized and evaluated the biological relevance of sulfated polysaccharides, which shows great potential in terms of immunological activity. Scope and approach Through a cr...
51 CitationsSource
#1Ling Chen (CNU: Chongqing Normal University)H-Index: 6
#2Gangliang Huang (CNU: Chongqing Normal University)H-Index: 18
Abstract Pumpkin polysaccharide was extracted by hot water extraction method. It was modified with chlorosulfonic acid-pyridine to obtain sulfated pumpkin polysaccharides (SP1, SP2) with different degrees of substitution, which was 0.35 and 0.65, respectively. The total sugar contents of pumpkin polysaccharide and its sulfated derivatives were determined by phenol‑sulfuric acid method, the ability of scavenging hydroxyl radicals and superoxide anions as well as the ability of reducing were deter...
31 CitationsSource
#1Surina Bo (Inner Mongolia Medical University)H-Index: 1
#2Mu Dan (Inner Mongolia Medical University)H-Index: 1
Last. Ping Zhang (Inner Mongolia Medical University)H-Index: 1
view all 4 authors...
Abstract A polysaccharide from Arnebia euchroma (Royle) Johnst. named ARP, was obtained and purified by the hot water extraction, ethanol precipitation and deproteinization of TCA. The molecular weight of the polysaccharide fraction of ARP was calculated to be 1.23 × 104 Da from a calibration curve obtained with dextran standards. Monosaccharide composition analysis revealed that ARP was composed of Gal, Ara, Glu, Man, Rha and Fuc at a molar ratio of 53.8:21.3:11.7:6.8:4.3:2.2. Methylation analy...
5 CitationsSource
#1Bao Peng (JNU: Jinan University)H-Index: 3
#2Yuanyuan Luo (JNU: Jinan University)H-Index: 2
Last. Rongmin Yu (JNU: Jinan University)H-Index: 19
view all 8 authors...
Abstract The homogeneous heteropolysaccharide CMSPW90-1 was first purified from bergamot by DEAE Sepharose Fast Flow and Sephadex G-75 column with a molecular weight of 18.8 kDa. The structure of CMSPW90-1 was elucidated with high-performance gel permeation chromatography, gas chromatography–mass spectrometry, infrared spectrum, methylation, nuclear magnetic resonance (NMR), Congo red test, and circular dichroism. In comparison with CMSPW90-1, the sulfated derivative CMSPW90-M1 showed significan...
15 CitationsSource
#1Liqiao Liu (NCU: Nanchang University)H-Index: 2
#2Mingzhu Li (NCU: Nanchang University)H-Index: 1
Last. Jianhua Xie (NCU: Nanchang University)H-Index: 35
view all 7 authors...
Abstract Natural polysaccharides, the abundant dietary components, show potent antitumor bioactivity in treatment and chemoprevention. Compared with traditional tumor therapy, natural polysaccharides are characterized by the low toxicity and immuno-regulation function. Recent researches have indicated that the anti-tumor of polysaccharides was partly attributable to modification in the composition of gut microbiota when polysaccharides were digested. Indeed, microbial dysbiosis or alteration of ...
54 CitationsSource
#1Fang Fang (Purdue University)H-Index: 8
#2Yunus E. Tuncil (Purdue University)H-Index: 10
Last. Osvaldo H. Campanella (Purdue University)H-Index: 36
view all 6 authors...
Abstract An unusual shear-thickening behavior was observed in 10% w/w gelatinized waxy potato and waxy corn starch dispersions at shear rates around 20 s−1. However, the phenomenon was not found in gelatinized dispersions of waxy rice. The aim of this study was to investigate reasons for the observed shear-thickening behavior as well as its relationship with the amylopectin molecular structure. This unique phenomenon only appears at a shear rate of about 20 s−1 during the increasing shear rate s...
14 CitationsSource
#1Fang Chen (CNU: Chongqing Normal University)H-Index: 9
#2Gangliang Huang (CNU: Chongqing Normal University)H-Index: 18
Abstract Polysaccharide is a kind of natural macromolecule polymer, which is widely existed in animals, plants and microorganisms, has a variety of biological activities, such as anti-tumor, anti-virus, anti-oxidation and immune regulation. Polysaccharides can affect macrophages, cells and other immune cells to improve the body's immune function, and have no toxic and side effects on normal cells, which make the polysaccharides receive widespread attention. In recent years, studies have shown th...
98 CitationsSource
#2Jianhua Xie (NCU: Nanchang University)H-Index: 35
#3Mingyue Shen (NCU: Nanchang University)H-Index: 29
Last. Mingyong Xie (NCU: Nanchang University)H-Index: 71
view all 5 authors...
Abstract Background Polysaccharides are a kind of biological macromolecular substance with multiple biological effects. Natural polysaccharides derived from plants and fungi are known as ideal raw food supplements for health food and pharmaceuticals due to their few side effects. Sulfated modification could significantly improve structure characteristics, promote bioactivities, and even add new bioactivities to polysaccharides. Thus, sulfated polysaccharides are increasingly causing more attenti...
85 CitationsSource
Cited By12
Newest
#1Muhammad Muneeb Ahmad (GCUF: Government College University, Faisalabad)H-Index: 1
6 CitationsSource
#7Zhang Jian (Nanjing University of Chinese Medicine)
Two previously undescribed triterpenoids (1-2), along with thirteen known compounds (3-15) were isolated from a CHCl3-soluble extract of the leaves of Cyclocarya paliurus. Their structures were established on the basis of chemical and spectroscopic approaches. These compounds were assessed for their therapeutic effects on diabetic nephropathy (DN)-evoked fibrosis through High-Glucose and transforming growth factor-β1 (TGF-β1) challenged HK-2 cells. Among them, compounds 3, 5 and 8 could remarked...
Source
#1Yanfei He (ZJU: Zhejiang University)
#2Haoxuan Peng (Beijing University of Chinese Medicine)
Last. Hongxiang Sun (ZJU: Zhejiang University)H-Index: 27
view all 5 authors...
Abstract The current experiments were designed to explore the structural features and immunopotentiation activity of two homogeneous polysaccharides PCSPA and PCSPB prepared from Crocus sativus petals using DEAE-Sephadex A-50 and Sephadex G200 column chromatography. The structures of PCSPA and PCSPB were systematically characterized using extensive chemical and spectroscopic methods including colorimetry, HPGPC-RID, GC–MS, Smith degradations, methylation, solvolytic desulfation, UV, FT-IR, NMR, ...
1 CitationsSource
#1Ting Wu (NCU: Nanchang University)H-Index: 5
#2Mingyue Shen (NCU: Nanchang University)H-Index: 29
Last. Jianhua Xie (NCU: Nanchang University)H-Index: 35
view all 9 authors...
Abstract The purpose of this paper was to investigate the effects of Cyclocarya paliurus polysaccharide (CP) on gut microbiota composition and predict metabolic function in healthy mice. Healthy Kunming mice were continuously gavaged with CP for 20 days, and mouse feces were collected for analysis. The results showed that CP could remarkably increase the short-chain fatty acids (SCFAs; acetic acid, propionic acid, butyric acid, and valeric acid) in the feces of healthy mice in a dose-dependent m...
Source
#1Øystein Arlov (SINTEF)H-Index: 9
#2Dominic Rütsche (ETH Zurich)
Last. Marcy Zenobi-Wong (ETH Zurich)H-Index: 31
view all 5 authors...
2 CitationsSource
#1Rong Huang (NCU: Nanchang University)H-Index: 2
#2Mingyue Shen (NCU: Nanchang University)H-Index: 29
Last. Jianhua Xie (NCU: Nanchang University)H-Index: 35
view all 5 authors...
Abstract A sulfated Chinese yam polysaccharide (S-CYP) was obtained from non-starch Chinese yam polysaccharide (CYP) by chlorosulfonic acid-pyridine (CSA-Pyr) method. The physicochemical characteristics and immunological activity of S-CYP were evaluated in this study. Results showed that sulfated modification changed the physicochemical properties of Chinese yam polysaccharide. Body weights and thymus index of the mice in polysaccharides group were increased compared with the cyclophosphamide (C...
7 CitationsSource
#1Hongyan Li (QUST: Qingdao University of Science and Technology)H-Index: 2
#2Wancui Xie (QUST: Qingdao University of Science and Technology)H-Index: 2
Last. Xihong Yang (QUST: Qingdao University of Science and Technology)H-Index: 4
view all 5 authors...
Abstract The immunomodulatory effects of the four extracellular polysaccharides, namely WPA, WPB, AP2A, and TP1A, which were isolated from the fermented broth of Aspergillus aculeatus, A. terreus and Trichoderma sp. KK19L1, were investigated in vitro. WPA, WPB, AP2A, and TP1A were not toxic to RAW264.7 cells. These polysaccharides enhanced cell viability. WPA, WPB, AP2A, and TP1A showed increased immunomodulatory effect by strengthening the phagocytic activity and enhancing the release of NO, TN...
4 CitationsSource
#1Ting Wu (NCU: Nanchang University)H-Index: 5
#2Mingyue Shen (NCU: Nanchang University)H-Index: 29
Last. Jianhua Xie (NCU: Nanchang University)H-Index: 35
view all 8 authors...
Abstract The study was to investigate the anti-inflammatory effect of Cyclocarya paliurus polysaccharides (CP) on carbon tetrachloride (CCl4) induced mice and explore its underlying mechanism. Based on a mouse model of CCl4 induced liver inflammation and pretreatment with CP. Results showed that CP significantly decreased the levels of the proinflammatory cytokines (NO, TNF-α, IL-1β) as well as the proteins expression levels of COX-2 and iNOS in the liver. TLR4 and MAPK pathways were down-regula...
11 CitationsSource
#1Yi Han (JXAU: Jiangxi Agricultural University)H-Index: 4
#2Kehui Ouyang (JXAU: Jiangxi Agricultural University)H-Index: 9
Last. Wenjun Wang (JXAU: Jiangxi Agricultural University)H-Index: 10
view all 12 authors...
Abstract In this study, a crude and purified polysaccharide from Cyclocarya paliurus (CPP, CPP0.05) were performed with chlorosulfonic acid–pyridine (CSA–Pyr) method to obtain sulfated derivatives (S-CPP, S-CPP0.05). After comparatively investigating, characterization results showed that the modifications were successful. Polysaccharides were used to culture mouse bone marrow-derived dendritic cells (BM-DCs) to evaluate their immunomodulatory activity and explore mechanism. The functional activi...
7 CitationsSource
#1Mohib Ullah Kakar (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 8
#2Muhammad Naveed (Nanjing Medical University)H-Index: 55
Last. Rongji Dai (BIT: Beijing Institute of Technology)H-Index: 17
view all 9 authors...
Cyclocarya paliurus is essential and only living specie of the genus Cyclocarya Iljinskaja. The leaves of this plant have been extensively used as food in the form of tea and green vegetable. Many compounds have been isolated from this plant, and their useful aspects explored, including the polysaccharides. Studies conducted on leaves show that different methods of extraction have been used, as well as a combination of different techniques that have been applied to isolate polysaccharides from t...
19 CitationsSource