Quantum mechanics

Biology

Materials science

Microscopy

Cryo-electron microscopy

Biochemistry

Computer science

Electron microscope

Model building

Resolution (logic)

Physics

Optics

Artificial intelligence

Macromolecule

Morphing

Crystallography

Chemistry

Biophysics

Protein Science

Coot is a tool widely used for model building, refinement, and validation of macromolecular structures. It has been extensively used for crystallography and, more recently, improvements have been introduced to aid in cryo-EM model building and refinement, as cryo-EM structures with resolution ranging 2.5–4 A are now routinely available. Model building into these maps can be time-consuming and requires experience in both biochemistry and building into low-resolution maps. To simplify and expedite the model building task, and minimize the needed expertise, new tools are being added in Coot. Some examples include morphing, Geman-McClure restraints, full-chain refinement, and Fourier-model based residue-type-specific Ramachandran restraints. Here, we present the current state-of-the-art in Coot usage.

Current developments in <i>Coot</i> for macromolecular model building of Electron Cryo‐microscopy and Crystallographic Data

Current developments in Coot for macromolecular model building of Electron Cryo‐microscopy and Crystallographic Data