Effect of surface morphologies and chemistry of paper on deposited collagen

Published on Aug 1, 2019in Applied Surface Science6.182
· DOI :10.1016/J.APSUSC.2019.04.131
Boyce S. Chang11
Estimated H-index: 11
(Iowa State University),
Anuraag Boddupalli4
Estimated H-index: 4
(Iowa State University)
+ 4 AuthorsMartin M. Thuo23
Estimated H-index: 23
(Iowa State University)
Sources
Abstract
Abstract Paper-based platforms for biological studies have received significant attention given that cellulose is ubiquitous, biocompatible, and can be readily organized into tunable fibrous structures. In the latter form, effect of complexity in surface morphologies (roughness, porosity and fiber organization) on cell-substrate interaction has not been thoroughly explored. We infer that altering the properties of a fibrous material should lead to significant changes in cellular microenvironment and direct the deposition of structurally analogous extracellular matrix (fiber-fiber templating) like collagen. Here, we elucidate the effect of varying paper roughness and surface chemistry on NIH/3T3 fibroblasts via organization of excreted collagen. Collagen intensity was found to increase linearly with paper porosity, indicating a 3D culture platform. The intensity, however, decays over time due to biodegradation of the substrate. Stability can be improved by introducing fluorinated alkyl silanes to yield hydrophobic paper. This process concomitantly transforms the substrate to a 2D-like scaffold where collagen is predominantly assembled on the surface, thus changing the cellular microenvironment. Altering surface energy also led to fluctuations in collagen intensity and organization over time for smooth (calendered) paper substrates. We infer that the increased roughness improves collagen adsorption through capillary driven petal effect. In general, the influence of the substrate simultaneously affects its ability to host collagen and guide orientation. These findings offer insights into the effects of secondary structures and chemistry of fibrous polymeric materials on cell culture, which we propose as vital parameters when using paper-based platforms.
📖 Papers frequently viewed together
2010
4 Authors (Andreas Stylianou, ..., Dido Yova)
12 Citations
9 Citations
28 Citations
References47
Newest
#1Juan Wang (Iowa State University)H-Index: 5
#2Anuraag Boddupalli (Iowa State University)H-Index: 4
Last. Ian C. Schneider (Iowa State University)H-Index: 14
view all 7 authors...
Introduction The extracellular matrix (ECM) in the tumor microenvironment contains high densities of collagen that are highly aligned, resulting in directional migration called contact guidance that facilitates efficient migration out of the tumor. Cancer cells can remodel the ECM through traction force controlled by myosin contractility or proteolytic activity controlled by matrix metalloproteinase (MMP) activity, leading to either enhanced or diminished contact guidance.
2 CitationsSource
#1Paul Gregory (Iowa State University)H-Index: 2
#2Andrew Martin (Iowa State University)H-Index: 7
Last. Martin M. Thuo (Iowa State University)H-Index: 23
view all 6 authors...
Fibrous cellulosic materials have been used as templates for material synthesis or organization via thermal degradation of the cellulose. Most of these methods, however, fail to exploit fiber organization, in part due to loss of structure with processing. Herein, we demonstrate that chemi- and physi-sorbed modifiers of cellulose alters the thermal degradation mechanism allowing for controlled deposition of oxide and carbon (incomplete combustion) along the original paper fiber network. We demons...
2 CitationsSource
#1Karla Esbona (UW: University of Wisconsin-Madison)H-Index: 9
#2Yanyao Yi (UW: University of Wisconsin-Madison)H-Index: 4
Last. Patricia J. Keely (UW: University of Wisconsin-Madison)H-Index: 53
view all 12 authors...
Inflammation, and the organization of collagen in the breast tumor microenvironment, is an important mediator of breast tumor progression. However, a direct link between markers of inflammation, collagen organization, and patient outcome has yet to be established. A tumor microarray of 371 invasive breast carcinoma biopsy specimens was analyzed for expression of inflammatory markers, including cyclooxygenase 2 (COX-2), macrophages, and several collagen features in the tumor nest (TN) or the tumo...
38 CitationsSource
#2Oscar del BarcoH-Index: 4
Last. Juan M. BuenoH-Index: 15
view all 3 authors...
Collagen organization has been analyzed at both external and internal scales by combining Stokes-vector polarimetry and second harmonic generation microscopy. A significant linear relationship between the diattenuation and the external collagen organization was found. The dominant orientation of the collagen fibers was found to run parallel to the axis of diattenuation. Information on the collagen chirality was obtained from the circular dichroism, which showed also a strong dependence with the ...
10 CitationsSource
#1Simone Birkheur (UEL: Universidade Estadual de Londrina)H-Index: 1
#2Paula C. S. Faria-Tischer (UEL: Universidade Estadual de Londrina)H-Index: 9
view all 8 authors...
Abstract Bacterial cellulose membrane is a biomaterial with high value in the biomedical field. Many groups have been making efforts to promote chemical modifications of its structure and, consequently, add new characteristics. Recently, our group has developed a methodology to insert monoester succinic acid in bacterial cellulose membrane without disrupting the microfibril network and bind a protein on it. Considering the role of carbohydrates in the molecular recognition process in biological ...
9 CitationsSource
#1Dionysios C. Christodouleas (Harvard University)H-Index: 17
#2Felice C. Simeone (Harvard University)H-Index: 7
Last. George M. Whitesides (Wyss Institute for Biologically Inspired Engineering)H-Index: 263
view all 8 authors...
This manuscript describes a simple and rapid method for fabricating free-standing structures composed primarily (>94% w/w, and 55–80 at%) of noble metals (e.g., gold, silver, platinum, etc.) and having physical morphologies that resemble paper, thread, or fabric. In this method, templates (i.e., pieces of paper, or cotton fabric) are loaded with aqueous solutions of salts of noble metals, and then the cellulosic component is burned off in a furnace held at high temperatures (i.e., from 550 °C to...
12 CitationsSource
Last. Zaver M. BhujwallaH-Index: 55
view all 10 authors...
// Balaji Krishnamachary 1, * , Ioannis Stasinopoulos 1, * , Samata Kakkad 1 , Marie-France Penet 1, 2 , Desmond Jacob 1 , Flonne Wildes 1 , Yelena Mironchik 1 , Arvind P. Pathak 1, 2 , Meiyappan Solaiyappan 1 , Zaver M. Bhujwalla 1, 2 1 JHU ICMIC Program, Division of Cancer Imaging Research, The Russell H. Morgan Department of Radiology and Radiological Science, Baltimore, MD 21205, USA 2 Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD 2...
23 CitationsSource
#1Stephanie Oyola-Reynoso (Iowa State University)H-Index: 12
#2Christophe Frankiewicz (Iowa State University)H-Index: 8
Last. Martin M. Thuo (Iowa State University)H-Index: 23
view all 6 authors...
We report a simple, efficient, one-step, affordable method to produce open-channel paper-based microfluidic channels. One surface of a sheet of paper is selectively calendared, with concomitant hydrophobization, to create the microfluidic channel. Our method involves asymmetric mechanical modification of a paper surface using a rolling ball (ball-point pen) under a controlled amount of applied stress (rz) to ascertain that only one side is modified. A lubricating solvent (hexane) aids in the sel...
10 CitationsSource
#1Kelvin Tsun Wai Ng (UM: University of Malaya)H-Index: 19
#1Kelvin Ng (Xi'an Jiaotong University)H-Index: 4
Last. Feng Xu (Xi'an Jiaotong University)H-Index: 96
view all 11 authors...
Paper has recently attracted increasing attention as a substrate for various biomedical applications. By modifying its physical and chemical properties, paper can be used as an alternative to conventional cell culture substrates. Because it can be stacked into a three-dimensional (3D) structure, which can better mimic the in vivo cell microenvironment. Paper has shown great potential as a 3D cell culture platform for developing normal and diseased models. This platform gives precise control over...
70 CitationsSource
#1Stephanie Oyola-Reynoso (Iowa State University)H-Index: 12
#2Dickson Kihereko (KU: Kenyatta University)H-Index: 1
Last. Margaret M. Ng’ang’a (KU: Kenyatta University)H-Index: 6
view all 8 authors...
Abstract The demand for rapid diagnostic in developing countries has recently increased, in part due to growing populations, emerging diseases, and rise in healthcare cost. Use of low-cost lateral flow/dipstick devices, especially paper-based ones, has increased. In most of the developing world, however, biomedical waste management systems either do not exist or are poor, as such, used devices either get incinerated or dumped alongside household trash. The plastic casing that is often used to ho...
6 CitationsSource
Cited By2
Newest
#1Marina Sala de Medeiros (Purdue University)H-Index: 3
#2Debkalpa Goswami (MIT: Massachusetts Institute of Technology)H-Index: 11
Last. Ramses V. Martinez (Purdue University)H-Index: 23
view all 5 authors...
Abstract The commercial development of smart garments is currently hindered by significant challenges, such as dependence on batteries, reduced washability, and difficult incorporation into existing large-scale textile manufacturing technologies. This work describes an industrially scalable approach to transform conventional fabrics into smart textiles—wirelessly powered by omniphobic silk-based coils (OSCs). OSCs are stretchable and lightweight power-receiving coils that can be easily sewn onto...
2 CitationsSource
#1Marina Sala de Medeiros (Purdue University)H-Index: 3
#2Daniela Chanci (Purdue University)H-Index: 2
Last. Ramses V. Martinez (Purdue University)H-Index: 23
view all 3 authors...
Abstract The fabrication of multifunctional electronic devices on ubiquitous paper substrates is gaining considerable attention due to their low cost, environmental friendliness, light weight, and flexibility. Unfortunately, the development of paper-based electronics is subject to significant challenges, such as rapid degradation with moisture, battery dependence, and limited compatibility with existing mass production technologies. This work describes omniphobic, self-powered paper-based electr...
6 CitationsSource