Physics

Kinetics

Organic chemistry

Protonation

Stereochemistry

Crystallography

Deprotonation

Chemistry

Moiety

Rhodopsin

Amide

Ouabain

Spectroscopy

Sodium

Retinal

Quantum mechanics

Sodium pump

Biochemistry

Photochemistry

Physical Chemistry Chemical Physics

We report a comparative study on the structural dynamics of the light-driven sodium pump Krokinobacter eikastus rhodopsin 2 wild type under sodium and proton pumping conditions by means of time-resolved IR spectroscopy. The kinetics of KR2 under sodium pumping conditions exhibits a sequential character, whereas the kinetics of KR2 under proton pumping conditions involves several equilibrium states. The sodium translocation itself is characterized by major conformational changes of the protein backbone, such as distortions of the α-helices and probably of the ECL1 domain, indicated by distinct marker bands in the amide I region. Carbonyl stretch modes of specific amino acid residues helped to elucidate structural changes in the retinal Schiff base moiety, including the protonation and deprotonation of D116, which is crucial for a deeper understanding of the mechanistic features in the photocycle of KR2.

Time-resolved IR spectroscopy reveals mechanistic details of ion transport in the sodium pump<i>Krokinobacter eikastus</i>rhodopsin 2

Time-resolved IR spectroscopy reveals mechanistic details of ion transport in the sodium pumpKrokinobacter eikastusrhodopsin 2