Anatomy

Biotechnology

In vivo

Bacteria

Biology

Microbiology

Dorsum

Metallurgy

Materials science

Nail plate

Ex vivo

Nail (fastener)

Paronychia

Fusarium oxysporum

Botany

Genetics

Biofilm

Future Microbiology

Aim: To evaluate and characterize the etiopathogenesis of the fusarial onychomycosis in an ex vivo study through fragments of sterile human nail, without the addition of any nutritional source. Materials & methods: The infection and invasion of Fusarium oxysporum in the nail were evaluated by scanning electron microscopy (SEM), CFU, matrix, histopathology and Fourier Transform Infrared Spectrometer coupled to an equipment with diamond accessory (FTIR-ATR). Results: F. oxysporum infected and invaded across the nail, regardless of application face. However, the dorsal nail surface was the strongest barrier, while the ventral was more vulnerable to infection and invasion process. The fungal–nail interaction resulted in the formation of a dense biofilm. Conclusion: F. oxysporum infect and invade the healthy human nail, resulting in biofilm formation. Therefore, F. oxysporum is likely a primary onychomycosis agent.

<i>Fusarium Oxysporum</i> is an Onychomycosis Etiopathogenic Agent

Fusarium Oxysporum is an Onychomycosis Etiopathogenic Agent