Catalysis

High-performance liquid chromatography

Chromatography

Molecularly imprinted polymer

Solid phase extraction

Sample preparation

Biochemistry

Solvent

Polymer

Response surface methodology

Extraction (chemistry)

Organic chemistry

Analyte

Copolymer

Central composite design

Chemistry

Selectivity

Detection limit

Acrylamide

Talanta

In this work, novel dummy molecularly imprinted polymers (DMIP) with propanamide as a dummy template molecule were prepared based on a green synthesis strategy of less consumption of hazardous/organic reagents and at mild conditions for magnetic solid-phase extraction (MSPE) of acrylamide in biscuit samples, followed by high performance liquid chromatography (HPLC) determination. The resultant DMIP was well characterized by FT-IR, SEM, TEM and VSM, exhibiting uniform nanoscale coreshell structure and good magnetic property in favor of simple rapid separation. Several main variables influencing MSPE efficiency were investigated, including DMIP dosage, sample solution pH, extraction time and desorption solvent; central composite design (CCD) and response surface methodology (RSM) were employed to assist in the MSPE condition optimization with rapidity and reliability. Under optimized conditions, excellent linearity for acrylamide was obtained in the range of 5.0-5000.0 µg kg-1, and low detection and quantification limits were 1.3 µg kg-1 and 4.4 µg kg-1, respectively. The method recoveries at five spiked concentrations were found within 86.0-98.3% with relative standard deviations (RSDs) of 1.2-4.1%. Furthermore, endogenous acrylamide was detected in five different biscuit samples and the RSDs values were lower than 3.3%. The present study suggested promising perspectives of water-compatible eco-friendly DMIP based MSPE-HPLC method for highly effective sample pretreatment and targeted analytes determination in complicated matrices.

Dummy molecularly imprinted polymers based on a green synthesis strategy for magnetic solid-phase extraction of acrylamide in food samples