Somatic hypermutation

Enzyme

Alternative splicing

Biology

Intron

Gene

Antibody

Activation-induced (cytidine) deaminase

Cytidine deaminase

B cell

Exon skipping

RNA splicing

Computational biology

Cytidine

CRISPR

Exon

Genetics

Biochemistry

Molecular Cell

RNA splicing is critical for gene expression, but difficult to investigate in their native context. Based on a CRISPR-guided cytidine deaminase, Yuan et al. have developed a universal approach to modulate splicing and to correct disease-associated defects. This technology will advance understanding of splicing and offer new opportunities for splicing-related diseases.

Genetic Modulation of RNA Splicing with a CRISPR-Guided Cytidine Deaminase