Biological system

Circular dichroism

Biology

Computer science

Optics

Protein structure

Quantum mechanics

Web server

The Internet

Biochemistry

Magnetic field

World Wide Web

Computational biology

Antiparallel (mathematics)

Synchrotron

Bioinformatics

Protein secondary structure

Crystallography

Fold (higher-order function)

Chemistry

Materials science

Programming language

Physics

Nucleic Acids Research

Circular dichroism (CD) spectroscopy is a widely used method to study the protein secondary structure. However, for decades, the general opinion was that the correct estimation of β-sheet content is challenging because of the large spectral and structural diversity of β-sheets. Recently, we showed that the orientation and twisting of β-sheets account for the observed spectral diversity, and developed a new method to estimate accurately the secondary structure (PNAS, 112, E3095). BeStSel web server provides the Beta Structure Selection method to analyze the CD spectra recorded by conventional or synchrotron radiation CD equipment. Both normalized and measured data can be uploaded to the server either as a single spectrum or series of spectra. The originality of BeStSel is that it carries out a detailed secondary structure analysis providing information on eight secondary structure components including parallel-β structure and antiparallel β-sheets with three different groups of twist. Based on these, it predic...

BeStSel: a web server for accurate protein secondary structure prediction and fold recognition from the circular dichroism spectra