Biology

Chromatography

Saponin

Biochemistry

Methanol

Solvent

Acetone

Phytochemical

Alternative medicine

Extraction (chemistry)

Organic chemistry

Antioxidant

Flavonoid

Rhizome

Medicine

Chemistry

Cyperus rotundus

Traditional medicine

Pathology

Pharmacognosy Magazine

Pharmacognosy Magazine,2018,14,54,261-267.DOI:10.4103/pm.pm_228_17Published:April 2018Type:Original ArticleAuthors:Arunagiri Kamala, Sushil Kumar Middha, Chitra Gopinath, HS Sindhura, and Chandrakant S Karigar Author(s) affiliations:Arunagiri Kamala1, Sushil Kumar Middha2, Chitra Gopinath3, HS Sindhura3, Chandrakant S Karigar4 1 Department of Studies and Research in Biochemistry, Tumkur University, Tumkur, Karnataka, India 2 Department of Biotechnology, Maharani Lakshmi Ammanni College for Women, Bengaluru, Karnataka, India 3 Department of Biochemistry, Maharani Lakshmi Ammanni College for Women, Bengaluru, Karnataka, India 4 Department of Studies and Research in Biochemistry, Tumkur University, Tumkur; Department of Biochemistry, Bangalore University, Bengaluru, Karnataka, India Abstract:Background: Cyperus rotundus L. (family Cyperaceae), native to India, is a multivalent medicinal plant widely used in conventional medicine. The research reports on bioactive components from C. rotundus L. are scanty. Objective: The objective of the study was to optimize the best solvent system and bioprospect the possible phytochemicals in C. rotundus L. rhizome (CRR). Materials and Methods: The phytochemicals were extracted from the rhizomes of C. rotundus L. by successive Soxhlet technique with solvents of increasing polarity. The resultant extracts were analyzed for their total flavonoid content (TFC), total phenolic content (TPC), total proanthocyanidin content (TPAC), in vitro antioxidant potential, and inhibition of lipid peroxidation. The 70% acetone extract of CRR was analyzed using gas chromatography–mass spectrometry (GC-MS) for probable phytochemicals. Results and Discussion: The TPC, TFC, and TPAC estimates ranged from 0.036 ± 0.002 to 118.924 ± 5.946 μg/mg extract, 7.196 ± 0.359 to 200.654 ± 10.032 μg/mg extract, and 13.115 ± 0.656 to 45.901 ± 2.295 μg/mg extract, respectively. The quantities of TPC, TFC, and TPAC were found to be the highest in 70% acetone extract. The 70% acetone and 70% methanol extracts revealed best radical scavenging effect. GC-MS analysis of CRR extract revealed the presence of a novel compound 1 (2)-acetyl-3 (5)-styryl-5 (3)-methylthiopyrazole. Conclusion: The study indicated that 70% acetone and 70% methanol extracts of CRRs can be a potential source of antioxidants. Keywords:Alkaloids, Antioxidants, Cyperus rotundus L. Rhizome, Gas chromatography-mass spectrometry analysis, Successive solvent extractionView:PDF (1.35 MB)

In vitro antioxidant potentials of Cyperus rotundus L. rhizome extracts and their phytochemical analysis