Patch clamp

Biotechnology

Neuroscience

Biology

Somatosensory system

Membrane potential

In vivo

Physics

Electrophysiology

Hippocampus

Tetrodotoxin

Condensed matter physics

Chemistry

Pyramidal cell

Biophysics

Conductance

Slice preparation

eNeuro

Synaptic activity in vivo can potentially alter the integration properties of neurons. Using recordings in awake mice, we targeted somatosensory layer 2/3 pyramidal neurons and compared neuronal properties with those from slices. Pyramidal cells in vivo had lower resistance and gain values, as well as broader spikes and increased spike frequency adaptation compared to the same cells in slices. Increasing conductance in neurons using dynamic clamp to levels observed in vivo, however, did not lessen the differences between in vivo and slice conditions. Further, local application of tetrodotoxin (TTX) in vivo blocked synaptic-mediated membrane voltage fluctuations but had little impact on pyramidal cell membrane input resistance and time constant values. Differences in electrophysiological properties of layer 2/3 neurons in mouse somatosensory cortex, therefore, stem from intrinsic sources separate from synaptic-mediated membrane voltage fluctuations.

Differences in the Electrophysiological Properties of Mouse Somatosensory Layer 2/3 Neurons<i>In Vivo</i>and Slice Stem from Intrinsic Sources Rather than a Network-Generated High Conductance State

Differences in the Electrophysiological Properties of Mouse Somatosensory Layer 2/3 NeuronsIn Vivoand Slice Stem from Intrinsic Sources Rather than a Network-Generated High Conductance State