Structural prediction of protein models using distance restraints derived from cross-linking mass spectrometry data

This protocol describes how to use distance restraints obtained from cross-linking mass spectrometry (XL-MS) experiments to guide the structural prediction of proteins and protein complexes. This protocol describes a workflow for creating structural models of proteins or protein complexes using distance restraints derived from cross-linking mass spectrometry experiments. The distance restraints are used (i) to adjust preliminary models that are...