Rapid in vitro shoot multiplication of the recalcitrant species Juglans nigra L.

Black walnut (Juglans nigra L.) has long been prized for its timber, leading to commercial cultivation and significant breeding efforts for improving marketable traits. Vegetative and in vitro black walnut propagation techniques, however, are variable and highly genotype dependent. Optimizing plant growth regulator type and concentration are integral for developing a successful micropropagation protocol. The addition of meta-topolin (MT)...