Fire blight

Gene

Pseudomonas

Enterobacter

Bacteria

Biology

Bacillus amyloliquefaciens

Bacterial disease

Biochemistry

Brassica

Botany

16S ribosomal RNA

Genetics

Serratia

Pantoea agglomerans

Brevibacterium

Pantoea

Microbiology

Escherichia coli

Paenibacillus polymyxa

Horticulture

Erwinia

Blackleg

Microorganism

Microbial Pathogenesis

The effectiveness of antagonistic bacteria to control Erwinia amylovora was evaluated under in vitro and field conditions. Among 61 bacteria isolated from soil and flowers of fire blight host plants of different Moroccan areas, 20 bacterial isolates showed higher antagonistic activity against the pathogen during agar-diffusion-test, attached blossoms assay and in a bioassay on immature pear fruits. Effective isolates were identified by using biochemical tests and 16 S rRNA genes sequencing. These isolates were grouped into the following genera: Alcaligenes (ACBC1), Pantoea (ACBC2, ACBP1, and ACBP2), Serratia (HC4), Brevibacterium (SF3, SF4, SF7, and SF15), Pseudomonas (SP9), and Bacillus (CPa12, CPa2, HF6, JB2, LMR2, SF14, SF16, SP10, SP13, and SP18). Furthermore, isolates were reported in the NCBI nucleotide sequence database (Genbank) under the accession numbers from KY357285 to KY357304. A 2-year field trials consisted of spray treatments with different bacterial antagonists was conducted on the susceptible apple cultivars 'Gala', 'Golden Parsi' and 'Golden Smoothee'. Their efficacies were evaluated 15 days post-inoculation on detached blossoms and were ranged from 54.6 to 95.0% for 11 strains, most of them were slightly better or better than that obtained with commercial bacterial strains P10c (66%) and QST713 (63%). In field trials, the most effective were P. agglomerans ACBP2, B. amyloliquefaciens LMR2, B. halotolerans (SF3 and SF4), and B. mojarvensis SF16. In addition, effective bacterial isolates did not show the pathogenicity signs towards plant tissue and are, therefore, considered as potential candidates to be integrated in actives ingredients of microbial formulation for the effective control of Fire Blight.

New potential bacterial antagonists for the biocontrol of fire blight disease (Erwinia amylovora) in Morocco