Organic chemistry

Enzyme

Thioredoxin

Gene

Protein sequencing

The Internet

Web server

World Wide Web

Solubility

Peptide sequence

Chemistry

Sequence (biology)

Computer science

Biochemistry

Bioinformatics

Protein solubility is an important property in industrial and therapeutic applications. Prediction is a challenge, despite a growing understanding of the relevant physicochemical properties.Protein-Sol is a web server for predicting protein solubility. Using available data for Escherichia coli protein solubility in a cell-free expression system, 35 sequence-based properties are calculated. Feature weights are determined from separation of low and high solubility subsets. The model returns a predicted solubility and an indication of the features which deviate most from average values. Two other properties are profiled in windowed calculation along the sequence: fold propensity, and net segment charge. The utility of these additional features is demonstrated with the example of thioredoxin.The Protein-Sol webserver is available at http://protein-sol.manchester.ac.uk.jim.warwicker@manchester.ac.uk.

Protein–Sol: a web tool for predicting protein solubility from sequence